On demand, wireless electrochemical release of brain derived neurotrophic factor
Organic conductive polymers are prime candidates for the on demand or controlled release of neurotrophic proteins which can enhance the electrode-neural interface. In this study, bipolar electrochemistry (BPE) is employed to provide a wireless electrical stimulation that avoids the need for the dire...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
|
| Series: | Electrochemistry Communications |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1388248123002011 |
| _version_ | 1827593627578138624 |
|---|---|
| author | Chunyan Qin Zhilian Yue Robert J. Forster Jun Chen Gordon G. Wallace |
| author_facet | Chunyan Qin Zhilian Yue Robert J. Forster Jun Chen Gordon G. Wallace |
| author_sort | Chunyan Qin |
| collection | DOAJ |
| description | Organic conductive polymers are prime candidates for the on demand or controlled release of neurotrophic proteins which can enhance the electrode-neural interface. In this study, bipolar electrochemistry (BPE) is employed to provide a wireless electrical stimulation that avoids the need for the direct physical connection necessary for conventional approaches. Brain-derived neurotrophic factor (BDNF) was incorporated into polypyrrole (PPy) with poly (2-methoxy-5 aniline sulfonic acid) (PMAS) as a dopant during the course of electrochemical synthesis. The synthetic PPy-PMAS-BDNF material acts as the bipolar electrode and is placed within an electric field generated by two driving electrodes. Controlled release of BDNF is demonstrated, which is wireless powered by BPE. This is likely due to the wirelessly activated redox reactions which induce gaps/channels within the structure. Quantification of the BDNF reveals significant differences in the controlled-release properties of the films driven by BPE compared to conventional wired electrochemistry. Human neuroblastoma cells (SH-SY5Y) cultured on the PPy-PMAS-BDNF electrode were subjected to one-week of wireless electrostimulation. Neurite outgrowth was significantly improved when the polymer containing BDNF and the film BPE stimulation. The data suggest that when the BPE is applied, the cells simultaneously respond to the wirelessly released BDNF and the wireless electrical stimulation through the bipolar electroactive polymer electrode. This synergistic effect promotes enhanced neurite outgrowth across the electrodes. |
| first_indexed | 2024-03-09T02:14:26Z |
| format | Article |
| id | doaj.art-2220928514f34215bbfa9d9632f4e5d7 |
| institution | Directory Open Access Journal |
| issn | 1388-2481 |
| language | English |
| last_indexed | 2024-03-09T02:14:26Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electrochemistry Communications |
| spelling | doaj.art-2220928514f34215bbfa9d9632f4e5d72023-12-07T05:28:05ZengElsevierElectrochemistry Communications1388-24812023-12-01157107626On demand, wireless electrochemical release of brain derived neurotrophic factorChunyan Qin0Zhilian Yue1Robert J. Forster2Jun Chen3Gordon G. Wallace4Intelligent Polymer Research Institute, Australian Institute for Innovative Materials, Innovation Campus, University of Wollongong, Squires Way, North Wollongong, NSW 2519, Australia; National Centre for Sensor Research, School of Chemical Sciences, Dublin City University, Dublin 9, IrelandIntelligent Polymer Research Institute, Australian Institute for Innovative Materials, Innovation Campus, University of Wollongong, Squires Way, North Wollongong, NSW 2519, Australia; Corresponding author.National Centre for Sensor Research, School of Chemical Sciences, Dublin City University, Dublin 9, IrelandIntelligent Polymer Research Institute, Australian Institute for Innovative Materials, Innovation Campus, University of Wollongong, Squires Way, North Wollongong, NSW 2519, AustraliaIntelligent Polymer Research Institute, Australian Institute for Innovative Materials, Innovation Campus, University of Wollongong, Squires Way, North Wollongong, NSW 2519, AustraliaOrganic conductive polymers are prime candidates for the on demand or controlled release of neurotrophic proteins which can enhance the electrode-neural interface. In this study, bipolar electrochemistry (BPE) is employed to provide a wireless electrical stimulation that avoids the need for the direct physical connection necessary for conventional approaches. Brain-derived neurotrophic factor (BDNF) was incorporated into polypyrrole (PPy) with poly (2-methoxy-5 aniline sulfonic acid) (PMAS) as a dopant during the course of electrochemical synthesis. The synthetic PPy-PMAS-BDNF material acts as the bipolar electrode and is placed within an electric field generated by two driving electrodes. Controlled release of BDNF is demonstrated, which is wireless powered by BPE. This is likely due to the wirelessly activated redox reactions which induce gaps/channels within the structure. Quantification of the BDNF reveals significant differences in the controlled-release properties of the films driven by BPE compared to conventional wired electrochemistry. Human neuroblastoma cells (SH-SY5Y) cultured on the PPy-PMAS-BDNF electrode were subjected to one-week of wireless electrostimulation. Neurite outgrowth was significantly improved when the polymer containing BDNF and the film BPE stimulation. The data suggest that when the BPE is applied, the cells simultaneously respond to the wirelessly released BDNF and the wireless electrical stimulation through the bipolar electroactive polymer electrode. This synergistic effect promotes enhanced neurite outgrowth across the electrodes.http://www.sciencedirect.com/science/article/pii/S1388248123002011On demand drug releaseWireless stimulationBipolar electrochemistryPolypyrroleBrain derived neurotrophic factorNeurite outgrowth |
| spellingShingle | Chunyan Qin Zhilian Yue Robert J. Forster Jun Chen Gordon G. Wallace On demand, wireless electrochemical release of brain derived neurotrophic factor Electrochemistry Communications On demand drug release Wireless stimulation Bipolar electrochemistry Polypyrrole Brain derived neurotrophic factor Neurite outgrowth |
| title | On demand, wireless electrochemical release of brain derived neurotrophic factor |
| title_full | On demand, wireless electrochemical release of brain derived neurotrophic factor |
| title_fullStr | On demand, wireless electrochemical release of brain derived neurotrophic factor |
| title_full_unstemmed | On demand, wireless electrochemical release of brain derived neurotrophic factor |
| title_short | On demand, wireless electrochemical release of brain derived neurotrophic factor |
| title_sort | on demand wireless electrochemical release of brain derived neurotrophic factor |
| topic | On demand drug release Wireless stimulation Bipolar electrochemistry Polypyrrole Brain derived neurotrophic factor Neurite outgrowth |
| url | http://www.sciencedirect.com/science/article/pii/S1388248123002011 |
| work_keys_str_mv | AT chunyanqin ondemandwirelesselectrochemicalreleaseofbrainderivedneurotrophicfactor AT zhilianyue ondemandwirelesselectrochemicalreleaseofbrainderivedneurotrophicfactor AT robertjforster ondemandwirelesselectrochemicalreleaseofbrainderivedneurotrophicfactor AT junchen ondemandwirelesselectrochemicalreleaseofbrainderivedneurotrophicfactor AT gordongwallace ondemandwirelesselectrochemicalreleaseofbrainderivedneurotrophicfactor |