Reversal of diabetic-induced myopathy by swimming exercise in pregnant rats: a translational intervention study
Abstract Gestational diabetes mellitus (GDM) plus rectus abdominis muscle (RAM) myopathy predicts long-term urinary incontinence (UI). Atrophic and stiff RAM are characteristics of diabetes-induced myopathy (DiM) in pregnant rats. This study aimed to determine whether swimming exercise (SE) has a th...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-10801-z |
| _version_ | 1817985369602260992 |
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| author | Bruna B. Catinelli Patrícia S. Rossignoli Juliana F. Floriano Aline M. Carr Rafael G. de Oliveira Nilton J. dos Santos Lara C. C. Úbeda Maria Angélica Spadella Raghavendra L. S. Hallur Luis Sobrevia Sérgio L. Felisbino Iracema M. P. Calderon Angélica M. P. Barbosa Marilza V. C. Rudge The Diamater Study Group |
| author_facet | Bruna B. Catinelli Patrícia S. Rossignoli Juliana F. Floriano Aline M. Carr Rafael G. de Oliveira Nilton J. dos Santos Lara C. C. Úbeda Maria Angélica Spadella Raghavendra L. S. Hallur Luis Sobrevia Sérgio L. Felisbino Iracema M. P. Calderon Angélica M. P. Barbosa Marilza V. C. Rudge The Diamater Study Group |
| author_sort | Bruna B. Catinelli |
| collection | DOAJ |
| description | Abstract Gestational diabetes mellitus (GDM) plus rectus abdominis muscle (RAM) myopathy predicts long-term urinary incontinence (UI). Atrophic and stiff RAM are characteristics of diabetes-induced myopathy (DiM) in pregnant rats. This study aimed to determine whether swimming exercise (SE) has a therapeutic effect in mild hyperglycemic pregnant rats model. We hypothesized that SE training might help to reverse RAM DiM. Mild hyperglycemic pregnant rats model was obtained by a unique subcutaneous injection of 100 mg/kg streptozotocin (diabetic group) or citrate buffer (non-diabetic group) on the first day of life in Wistar female newborns. At 90 days of life, the rats are mated and randomly allocated to remain sedentary or subjected to a SE protocol. The SE protocol started at gestational day 0 and consisted of 60 min/day for 6 days/week in a period of 20 days in a swim tunnel. On day 21, rats were sacrificed, and RAM was collected and studied by picrosirius red, immunohistochemistry, and transmission electron microscopy. The SE protocol increased the fiber area and diameter, and the slow-twitch and fast-twitch fiber area and diameter in the diabetic exercised group, a finding was also seen in control sedentary animals. There was a decreased type I collagen but not type III collagen area and showed a similar type I/type III ratio compared with the control sedentary group. In conclusion, SE during pregnancy reversed the RAM DiM in pregnant rats. These findings may be a potential protocol to consider in patients with RAM damage caused by GDM. |
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| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T23:57:13Z |
| publishDate | 2022-05-01 |
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| spelling | doaj.art-2221850599ec4a5cbdee0cd8278cd5e42022-12-22T02:23:51ZengNature PortfolioScientific Reports2045-23222022-05-0112111210.1038/s41598-022-10801-zReversal of diabetic-induced myopathy by swimming exercise in pregnant rats: a translational intervention studyBruna B. Catinelli0Patrícia S. Rossignoli1Juliana F. Floriano2Aline M. Carr3Rafael G. de Oliveira4Nilton J. dos Santos5Lara C. C. Úbeda6Maria Angélica Spadella7Raghavendra L. S. Hallur8Luis Sobrevia9Sérgio L. Felisbino10Iracema M. P. Calderon11Angélica M. P. Barbosa12Marilza V. C. Rudge13The Diamater Study Group14Postgraduate Program on Tocogynecology, Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (UNESP)Department of Physiotherapy and Occupational Therapy, School of Philosophy and Sciences, São Paulo State University (UNESP)Postgraduate Program on Tocogynecology, Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (UNESP)Postgraduate Program on Tocogynecology, Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (UNESP)Postgraduate Program on Tocogynecology, Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (UNESP)Laboratory of Extracellular Matrix Biology, Department of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University (UNESP)University of Marília (UNIMAR)Human Embryology Laboratory, Marília Medical School (FAMEMA)Postgraduate Program on Tocogynecology, Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (UNESP)Postgraduate Program on Tocogynecology, Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (UNESP)Laboratory of Extracellular Matrix Biology, Department of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University (UNESP)Postgraduate Program on Tocogynecology, Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (UNESP)Postgraduate Program on Tocogynecology, Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (UNESP)Postgraduate Program on Tocogynecology, Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (UNESP)Postgraduate Program on Tocogynecology, Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University (UNESP)Abstract Gestational diabetes mellitus (GDM) plus rectus abdominis muscle (RAM) myopathy predicts long-term urinary incontinence (UI). Atrophic and stiff RAM are characteristics of diabetes-induced myopathy (DiM) in pregnant rats. This study aimed to determine whether swimming exercise (SE) has a therapeutic effect in mild hyperglycemic pregnant rats model. We hypothesized that SE training might help to reverse RAM DiM. Mild hyperglycemic pregnant rats model was obtained by a unique subcutaneous injection of 100 mg/kg streptozotocin (diabetic group) or citrate buffer (non-diabetic group) on the first day of life in Wistar female newborns. At 90 days of life, the rats are mated and randomly allocated to remain sedentary or subjected to a SE protocol. The SE protocol started at gestational day 0 and consisted of 60 min/day for 6 days/week in a period of 20 days in a swim tunnel. On day 21, rats were sacrificed, and RAM was collected and studied by picrosirius red, immunohistochemistry, and transmission electron microscopy. The SE protocol increased the fiber area and diameter, and the slow-twitch and fast-twitch fiber area and diameter in the diabetic exercised group, a finding was also seen in control sedentary animals. There was a decreased type I collagen but not type III collagen area and showed a similar type I/type III ratio compared with the control sedentary group. In conclusion, SE during pregnancy reversed the RAM DiM in pregnant rats. These findings may be a potential protocol to consider in patients with RAM damage caused by GDM.https://doi.org/10.1038/s41598-022-10801-z |
| spellingShingle | Bruna B. Catinelli Patrícia S. Rossignoli Juliana F. Floriano Aline M. Carr Rafael G. de Oliveira Nilton J. dos Santos Lara C. C. Úbeda Maria Angélica Spadella Raghavendra L. S. Hallur Luis Sobrevia Sérgio L. Felisbino Iracema M. P. Calderon Angélica M. P. Barbosa Marilza V. C. Rudge The Diamater Study Group Reversal of diabetic-induced myopathy by swimming exercise in pregnant rats: a translational intervention study Scientific Reports |
| title | Reversal of diabetic-induced myopathy by swimming exercise in pregnant rats: a translational intervention study |
| title_full | Reversal of diabetic-induced myopathy by swimming exercise in pregnant rats: a translational intervention study |
| title_fullStr | Reversal of diabetic-induced myopathy by swimming exercise in pregnant rats: a translational intervention study |
| title_full_unstemmed | Reversal of diabetic-induced myopathy by swimming exercise in pregnant rats: a translational intervention study |
| title_short | Reversal of diabetic-induced myopathy by swimming exercise in pregnant rats: a translational intervention study |
| title_sort | reversal of diabetic induced myopathy by swimming exercise in pregnant rats a translational intervention study |
| url | https://doi.org/10.1038/s41598-022-10801-z |
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