Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses
Abstract Background Combined chondroitin sulfate (CS) and glucosamine (GlcN) has been widely used in oral formulations to prevent and treat osteoarthritis. CS is effective for controlling pain in osteoarthritic patients, whereas GlcN can stimulate glycosaminoglycan synthesis, thus reducing extracell...
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-022-03323-3 |
| _version_ | 1818467958560653312 |
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| author | Ana Lucia Miluzzi Yamada Cynthia do Prado Vendruscolo Marília Ferrari Marsiglia Eric Danilo Pauls Sotelo Fernanda Rodrigues Agreste Sarah Raphaela Torquato Seidel Joice Fülber Raquel Yvonne Arantes Baccarin Luis Claudio Lopes Correia da Silva |
| author_facet | Ana Lucia Miluzzi Yamada Cynthia do Prado Vendruscolo Marília Ferrari Marsiglia Eric Danilo Pauls Sotelo Fernanda Rodrigues Agreste Sarah Raphaela Torquato Seidel Joice Fülber Raquel Yvonne Arantes Baccarin Luis Claudio Lopes Correia da Silva |
| author_sort | Ana Lucia Miluzzi Yamada |
| collection | DOAJ |
| description | Abstract Background Combined chondroitin sulfate (CS) and glucosamine (GlcN) has been widely used in oral formulations to prevent and treat osteoarthritis. CS is effective for controlling pain in osteoarthritic patients, whereas GlcN can stimulate glycosaminoglycan synthesis, thus reducing extracellular matrix degradation. Although several studies have been published on this topic, the effectiveness of treatment with oral CS and GlcN remains uncertain. The objective of this study was to analyze the progression of experimentally induced osteoarthritis in horses and verify the effectiveness of an oral compound based on CS and GlcN to treat and/or modulate this disease. The study analyzed the metacarpophalangeal joint of the left thoracic limb of 16 horses divided into two groups, with eight horses treated with CS and GlcN in the treated group (GT) and eight untreated horses in the control group (GC). Chondral lesions were induced through arthroscopy, which was defined as time-point zero (T0). Physical, ultrasonographic, and radiographic examinations and synovial fluid biomarkers measurements were performed on days 0, 30, 60, 90, and 120. At the end of the experiment (T4), arthroscopy was performed again to macroscopically evaluate the joints and collect material for microscopic analysis. Results Significant differences were observed between groups in some evaluated parameters, such as visual lameness assessment, synovial concentrations of prostaglandin E2, and ultrasound examination. However, the GT still presented slightly improved results for joint flexion angle, analysis of lameness using sensors, and histopathological analysis of chondral repair tissue, however, without the statistical significance (p>0.05). Conclusions The treatment was considered effective in the clinical modulation of experimental osteoarthritis, with improvement of some parameters in the GT. However, this type of treatment may not be entirely effective to change the catabolic process in articular cartilage and the progressive induced chondral damage. |
| first_indexed | 2024-04-13T21:07:21Z |
| format | Article |
| id | doaj.art-22237a365ede4dbd8cf11e16c7d92aae |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-13T21:07:21Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-22237a365ede4dbd8cf11e16c7d92aae2022-12-22T02:29:55ZengBMCBMC Veterinary Research1746-61482022-06-0118111410.1186/s12917-022-03323-3Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horsesAna Lucia Miluzzi Yamada0Cynthia do Prado Vendruscolo1Marília Ferrari Marsiglia2Eric Danilo Pauls Sotelo3Fernanda Rodrigues Agreste4Sarah Raphaela Torquato Seidel5Joice Fülber6Raquel Yvonne Arantes Baccarin7Luis Claudio Lopes Correia da Silva8Department of Surgery, School of Veterinary Medicine and Animal Science, University of São PauloDepartment of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São PauloDepartment of Surgery, School of Veterinary Medicine and Animal Science, University of São PauloDepartment of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São PauloDepartment of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São PauloDepartment of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São PauloDepartment of Surgery, School of Veterinary Medicine and Animal Science, University of São PauloDepartment of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São PauloDepartment of Surgery, School of Veterinary Medicine and Animal Science, University of São PauloAbstract Background Combined chondroitin sulfate (CS) and glucosamine (GlcN) has been widely used in oral formulations to prevent and treat osteoarthritis. CS is effective for controlling pain in osteoarthritic patients, whereas GlcN can stimulate glycosaminoglycan synthesis, thus reducing extracellular matrix degradation. Although several studies have been published on this topic, the effectiveness of treatment with oral CS and GlcN remains uncertain. The objective of this study was to analyze the progression of experimentally induced osteoarthritis in horses and verify the effectiveness of an oral compound based on CS and GlcN to treat and/or modulate this disease. The study analyzed the metacarpophalangeal joint of the left thoracic limb of 16 horses divided into two groups, with eight horses treated with CS and GlcN in the treated group (GT) and eight untreated horses in the control group (GC). Chondral lesions were induced through arthroscopy, which was defined as time-point zero (T0). Physical, ultrasonographic, and radiographic examinations and synovial fluid biomarkers measurements were performed on days 0, 30, 60, 90, and 120. At the end of the experiment (T4), arthroscopy was performed again to macroscopically evaluate the joints and collect material for microscopic analysis. Results Significant differences were observed between groups in some evaluated parameters, such as visual lameness assessment, synovial concentrations of prostaglandin E2, and ultrasound examination. However, the GT still presented slightly improved results for joint flexion angle, analysis of lameness using sensors, and histopathological analysis of chondral repair tissue, however, without the statistical significance (p>0.05). Conclusions The treatment was considered effective in the clinical modulation of experimental osteoarthritis, with improvement of some parameters in the GT. However, this type of treatment may not be entirely effective to change the catabolic process in articular cartilage and the progressive induced chondral damage.https://doi.org/10.1186/s12917-022-03323-3EquineGlycosaminoglycanJointLamenessOsteoarthritis |
| spellingShingle | Ana Lucia Miluzzi Yamada Cynthia do Prado Vendruscolo Marília Ferrari Marsiglia Eric Danilo Pauls Sotelo Fernanda Rodrigues Agreste Sarah Raphaela Torquato Seidel Joice Fülber Raquel Yvonne Arantes Baccarin Luis Claudio Lopes Correia da Silva Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses BMC Veterinary Research Equine Glycosaminoglycan Joint Lameness Osteoarthritis |
| title | Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses |
| title_full | Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses |
| title_fullStr | Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses |
| title_full_unstemmed | Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses |
| title_short | Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses |
| title_sort | effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses |
| topic | Equine Glycosaminoglycan Joint Lameness Osteoarthritis |
| url | https://doi.org/10.1186/s12917-022-03323-3 |
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