KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media
Cultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases in vitro while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and the...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Japan Poultry Science Association
2021-10-01
|
| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/58/4/58_0200085/_html/-char/en |
| _version_ | 1797817053068918784 |
|---|---|
| author | Masafumi Katayama Manabu Onuma Tomokazu Fukuda |
| author_facet | Masafumi Katayama Manabu Onuma Tomokazu Fukuda |
| author_sort | Masafumi Katayama |
| collection | DOAJ |
| description | Cultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases in vitro while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and there are currently relatively few options for this basal medium (medium 199 and KAv-1). This means that there is still room for the development of an optimal basal medium for avian cell culture. Here we compare KAv-1 medium, Dulbecco's modified Eagle medium (DMEM) and medium 199 during the culture of chick fibroblasts and determine that KAv-1 remains the optimal medium for these assays. Our results show that DNA damage is reduced in fibroblasts cultured in the KAv-1 medium, when compared to both DMEM and Medium 199 and that these cells also display improved growth dynamics in KAv-1 medium when compared to both DMEM and medium 199. To the best of our knowledge, this is the first study to describe a comparative analysis of culture media for avian cells, which would provide useful information for poultry scientists. |
| first_indexed | 2024-03-13T08:46:45Z |
| format | Article |
| id | doaj.art-2224523949ed47ebb692c66663250a2f |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:46:45Z |
| publishDate | 2021-10-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-2224523949ed47ebb692c66663250a2f2023-05-30T00:51:47ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862021-10-0158427027910.2141/jpsa.0200085jpsaKAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 MediaMasafumi Katayama0Manabu Onuma1Tomokazu Fukuda2Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental StudiesCenter for Environmental Biology and Ecosystem Studies, National Institute for Environmental StudiesWildlife Genome Collaborative Research Group, National Institute for Environmental StudiesCultured cells are a useful resource for poultry scientists, since these cells allow scientists to evaluate biological responses to conditions such as infectious diseases in vitro while mimicking the whole-body response in birds. However avian cell culture requires an optimized basal medium, and there are currently relatively few options for this basal medium (medium 199 and KAv-1). This means that there is still room for the development of an optimal basal medium for avian cell culture. Here we compare KAv-1 medium, Dulbecco's modified Eagle medium (DMEM) and medium 199 during the culture of chick fibroblasts and determine that KAv-1 remains the optimal medium for these assays. Our results show that DNA damage is reduced in fibroblasts cultured in the KAv-1 medium, when compared to both DMEM and Medium 199 and that these cells also display improved growth dynamics in KAv-1 medium when compared to both DMEM and medium 199. To the best of our knowledge, this is the first study to describe a comparative analysis of culture media for avian cells, which would provide useful information for poultry scientists.https://www.jstage.jst.go.jp/article/jpsa/58/4/58_0200085/_html/-char/encell culturecell growthcellular senescencechick fibroblastsdna damagegrowth medium |
| spellingShingle | Masafumi Katayama Manabu Onuma Tomokazu Fukuda KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media The Journal of Poultry Science cell culture cell growth cellular senescence chick fibroblasts dna damage growth medium |
| title | KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media |
| title_full | KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media |
| title_fullStr | KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media |
| title_full_unstemmed | KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media |
| title_short | KAv-1 is Better Suited to Chick Fibroblast Culture than DMEM or 199 Media |
| title_sort | kav 1 is better suited to chick fibroblast culture than dmem or 199 media |
| topic | cell culture cell growth cellular senescence chick fibroblasts dna damage growth medium |
| url | https://www.jstage.jst.go.jp/article/jpsa/58/4/58_0200085/_html/-char/en |
| work_keys_str_mv | AT masafumikatayama kav1isbettersuitedtochickfibroblastculturethandmemor199media AT manabuonuma kav1isbettersuitedtochickfibroblastculturethandmemor199media AT tomokazufukuda kav1isbettersuitedtochickfibroblastculturethandmemor199media |