Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain
The heart has a dynamic mechanical environment contributed by its unique cellular composition and the resultant complex tissue structure. In pathological heart tissue, both the mechanics and cell composition can change and influence each other. As a result, the interplay between the cell phenotype a...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/11/3199 |
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| author | Richard Duc Hien Tran Tessa Altair Morris Daniela Gonzalez Ali Hatem Salaheldin Hassan Ahmed Hetta Anna Grosberg |
| author_facet | Richard Duc Hien Tran Tessa Altair Morris Daniela Gonzalez Ali Hatem Salaheldin Hassan Ahmed Hetta Anna Grosberg |
| author_sort | Richard Duc Hien Tran |
| collection | DOAJ |
| description | The heart has a dynamic mechanical environment contributed by its unique cellular composition and the resultant complex tissue structure. In pathological heart tissue, both the mechanics and cell composition can change and influence each other. As a result, the interplay between the cell phenotype and mechanical stimulation needs to be considered to understand the biophysical cell interactions and organization in healthy and diseased myocardium. In this work, we hypothesized that the overall tissue organization is controlled by varying densities of cardiomyocytes and fibroblasts in the heart. In order to test this hypothesis, we utilized a combination of mechanical strain, co-cultures of different cell types, and inhibitory drugs that block intercellular junction formation. To accomplish this, an image analysis pipeline was developed to automatically measure cell type-specific organization relative to the stretch direction. The results indicated that cardiac cell type-specific densities influence the overall organization of heart tissue such that it is possible to model healthy and fibrotic heart tissue in vitro. This study provides insight into how to mimic the dynamic mechanical environment of the heart in engineered tissue as well as providing valuable information about the process of cardiac remodeling and repair in diseased hearts. |
| first_indexed | 2024-03-10T05:36:04Z |
| format | Article |
| id | doaj.art-22261ae217ab43078f7ab74e0860efd7 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T05:36:04Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-22261ae217ab43078f7ab74e0860efd72023-11-22T22:52:45ZengMDPI AGCells2073-44092021-11-011011319910.3390/cells10113199Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic StrainRichard Duc Hien Tran0Tessa Altair Morris1Daniela Gonzalez2Ali Hatem Salaheldin Hassan Ahmed Hetta3Anna Grosberg4Edwards Lifesciences Center for Advanced Cardiovascular Technology, University of California, Irvine, CA 92617-2700, USAEdwards Lifesciences Center for Advanced Cardiovascular Technology, University of California, Irvine, CA 92617-2700, USAEdwards Lifesciences Center for Advanced Cardiovascular Technology, University of California, Irvine, CA 92617-2700, USAEdwards Lifesciences Center for Advanced Cardiovascular Technology, University of California, Irvine, CA 92617-2700, USAEdwards Lifesciences Center for Advanced Cardiovascular Technology, University of California, Irvine, CA 92617-2700, USAThe heart has a dynamic mechanical environment contributed by its unique cellular composition and the resultant complex tissue structure. In pathological heart tissue, both the mechanics and cell composition can change and influence each other. As a result, the interplay between the cell phenotype and mechanical stimulation needs to be considered to understand the biophysical cell interactions and organization in healthy and diseased myocardium. In this work, we hypothesized that the overall tissue organization is controlled by varying densities of cardiomyocytes and fibroblasts in the heart. In order to test this hypothesis, we utilized a combination of mechanical strain, co-cultures of different cell types, and inhibitory drugs that block intercellular junction formation. To accomplish this, an image analysis pipeline was developed to automatically measure cell type-specific organization relative to the stretch direction. The results indicated that cardiac cell type-specific densities influence the overall organization of heart tissue such that it is possible to model healthy and fibrotic heart tissue in vitro. This study provides insight into how to mimic the dynamic mechanical environment of the heart in engineered tissue as well as providing valuable information about the process of cardiac remodeling and repair in diseased hearts.https://www.mdpi.com/2073-4409/10/11/3199heart tissue organizationcell type classificationcyclic strainintercellular junctions |
| spellingShingle | Richard Duc Hien Tran Tessa Altair Morris Daniela Gonzalez Ali Hatem Salaheldin Hassan Ahmed Hetta Anna Grosberg Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain Cells heart tissue organization cell type classification cyclic strain intercellular junctions |
| title | Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain |
| title_full | Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain |
| title_fullStr | Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain |
| title_full_unstemmed | Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain |
| title_short | Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain |
| title_sort | quantitative evaluation of cardiac cell interactions and responses to cyclic strain |
| topic | heart tissue organization cell type classification cyclic strain intercellular junctions |
| url | https://www.mdpi.com/2073-4409/10/11/3199 |
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