Evaluation of Cyclin D1 Expression in Head and Neck Squamous Cell Carcinoma
Introdution: Squamous Cell Carcinoma (SCC) is an aggressive epithelial malignancy of the upper aero digestive tract and comprises 90% of all Head and Neck Squamous Cell Carcinoma (HNSCC). It is the sixth leading cancer worldwide with approximately 600,000 cases reported annually. It is one of t...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2017-02-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/9329/21760_CE[Ra]_F(Sh)_PF1(AH_RK)_PFA(AK)_PF2(P_PC)_PF3(AG_OM).pdf |
| _version_ | 1818558998577676288 |
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| author | Vishal Dhingra Jyoti Verma Vatsala Misra Sapan Srivastav Faheema Hasan |
| author_facet | Vishal Dhingra Jyoti Verma Vatsala Misra Sapan Srivastav Faheema Hasan |
| author_sort | Vishal Dhingra |
| collection | DOAJ |
| description | Introdution: Squamous Cell Carcinoma (SCC) is an aggressive
epithelial malignancy of the upper aero digestive tract
and comprises 90% of all Head and Neck Squamous Cell
Carcinoma (HNSCC). It is the sixth leading cancer worldwide
with approximately 600,000 cases reported annually. It is one
of the most common cancers in India. Tumour Lymph Node
and Metastases (TNM) staging has been the most useful
indicator to predict prognosis in HNSCC but recently various
biomolecular markers have potentially offered new methods for
early diagnosis and treatment alternatives for HNSCC patients;
one amongst them being cyclin D1.
Aim: This study has been undertaken to evaluate expression of
cyclin D1 in HNSCC cases and to find out its association with
various pathological prognostic factors.
Materials and Methods: A 48 formalin fixed paraffin embedded
tumour sections, stained with Haematoxylin & Eosin were
graded and staged. Immunohistochemistry for cyclin D1 was
evaluated as Extent Score (ES), Intensity Score (IS) and Total
Score (TS) was calculated.
Statistical Analysis: All the relevant data collected was
transferred on to the excel sheet. Chi square test with and without
Yate's correction was used to compare various parameters. The
p-value ≤ 0.05 was taken as critical level of significance.
Results: A significant association was seen between TS of
Cyclin D1 expression with tumour stage and with lymph node
metastasis but not with grade.
Conclusion: Higher Cyclin D1 expression is associated with
higher tumour stage and lymph node metastasis. Therefore,
there is value of analysing cyclin D1 amplification and
expression, for prognostic evaluation. This may also be further
used for targeted therapy in head and neck cancers. |
| first_indexed | 2024-12-14T00:19:37Z |
| format | Article |
| id | doaj.art-2226dc9a57c74acaaddfd9de2f819476 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-14T00:19:37Z |
| publishDate | 2017-02-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-2226dc9a57c74acaaddfd9de2f8194762022-12-21T23:25:17ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-02-01112EC01EC0410.7860/JCDR/2017/21760.9329Evaluation of Cyclin D1 Expression in Head and Neck Squamous Cell CarcinomaVishal Dhingra0Jyoti Verma1Vatsala Misra2Sapan Srivastav3Faheema Hasan4Assistant Professor, Department of Pathology, M.L.N Medical College, Allahabad, Uttar Pradesh, India.Student, Department of Pathology, M.L.N Medical College, Allahabad, Uttar Pradesh, India.Consultant Oncosurgeon, Department of Pathology, M.L.N Medical College, Allahabad, Uttar Pradesh, India.Professor, Department of Pathology, M.L.N Medical College, Allahabad, Uttar Pradesh, India.Student, Department of Pathology, M.L.N Medical College, Allahabad, Uttar Pradesh, India.Introdution: Squamous Cell Carcinoma (SCC) is an aggressive epithelial malignancy of the upper aero digestive tract and comprises 90% of all Head and Neck Squamous Cell Carcinoma (HNSCC). It is the sixth leading cancer worldwide with approximately 600,000 cases reported annually. It is one of the most common cancers in India. Tumour Lymph Node and Metastases (TNM) staging has been the most useful indicator to predict prognosis in HNSCC but recently various biomolecular markers have potentially offered new methods for early diagnosis and treatment alternatives for HNSCC patients; one amongst them being cyclin D1. Aim: This study has been undertaken to evaluate expression of cyclin D1 in HNSCC cases and to find out its association with various pathological prognostic factors. Materials and Methods: A 48 formalin fixed paraffin embedded tumour sections, stained with Haematoxylin & Eosin were graded and staged. Immunohistochemistry for cyclin D1 was evaluated as Extent Score (ES), Intensity Score (IS) and Total Score (TS) was calculated. Statistical Analysis: All the relevant data collected was transferred on to the excel sheet. Chi square test with and without Yate's correction was used to compare various parameters. The p-value ≤ 0.05 was taken as critical level of significance. Results: A significant association was seen between TS of Cyclin D1 expression with tumour stage and with lymph node metastasis but not with grade. Conclusion: Higher Cyclin D1 expression is associated with higher tumour stage and lymph node metastasis. Therefore, there is value of analysing cyclin D1 amplification and expression, for prognostic evaluation. This may also be further used for targeted therapy in head and neck cancers.https://jcdr.net/articles/PDF/9329/21760_CE[Ra]_F(Sh)_PF1(AH_RK)_PFA(AK)_PF2(P_PC)_PF3(AG_OM).pdfexpression scorehaematoxylin & eosinimmunohistochemistryintensity scoretotal score |
| spellingShingle | Vishal Dhingra Jyoti Verma Vatsala Misra Sapan Srivastav Faheema Hasan Evaluation of Cyclin D1 Expression in Head and Neck Squamous Cell Carcinoma Journal of Clinical and Diagnostic Research expression score haematoxylin & eosin immunohistochemistry intensity score total score |
| title | Evaluation of Cyclin D1 Expression in Head and Neck Squamous Cell Carcinoma |
| title_full | Evaluation of Cyclin D1 Expression in Head and Neck Squamous Cell Carcinoma |
| title_fullStr | Evaluation of Cyclin D1 Expression in Head and Neck Squamous Cell Carcinoma |
| title_full_unstemmed | Evaluation of Cyclin D1 Expression in Head and Neck Squamous Cell Carcinoma |
| title_short | Evaluation of Cyclin D1 Expression in Head and Neck Squamous Cell Carcinoma |
| title_sort | evaluation of cyclin d1 expression in head and neck squamous cell carcinoma |
| topic | expression score haematoxylin & eosin immunohistochemistry intensity score total score |
| url | https://jcdr.net/articles/PDF/9329/21760_CE[Ra]_F(Sh)_PF1(AH_RK)_PFA(AK)_PF2(P_PC)_PF3(AG_OM).pdf |
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