FTIR Spectroscopy for Identification and Intra-Species Characterization of <i>Serpula lacrymans</i>
The dry rot fungus <i>Serpula lacrymans</i> is the most destructive fungal agent of wood building materials in Europe, Russia, North America, and Japan. The identification of this wood-deteriorating agent and the discrimination of different fungal isolates is very important for the contr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/18/8463 |
| _version_ | 1797520372182024192 |
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| author | Rony Barboux Faisl Bousta Patrick Di Martino |
| author_facet | Rony Barboux Faisl Bousta Patrick Di Martino |
| author_sort | Rony Barboux |
| collection | DOAJ |
| description | The dry rot fungus <i>Serpula lacrymans</i> is the most destructive fungal agent of wood building materials in Europe, Russia, North America, and Japan. The identification of this wood-deteriorating agent and the discrimination of different fungal isolates is very important for the control of buildings in general and for the preservation of cultural heritage in particular. The objective of the study was to develop a Fourier transform infrared (FTIR) method coupled with a partial least square discriminant analysis (PLS-DA) for the sample preparation and identification of <i>S. lacrymans</i>. Five distinct <i>S. lacrymans</i> strains were analysed and compared to two strains of unrelated fungal species. Different methods of mycelial growth, sample preparation, and FTIR spectral data normalisation were compared. FTIR analysis of a harvested mycelium grown on the surface of a polyether sulfone microfiltration membrane deposited on a malt extract agar medium, followed by vector normalization and PLS-DA statistical analysis, resulted in 100% correct attribution at phylum, species, and strain level, regardless of the type of standardization used. This study confirms the applicability of FTIR spectroscopy for the identification of <i>S. lacrymans</i> and the discrimination of different strains belonging to this species. |
| first_indexed | 2024-03-10T07:55:50Z |
| format | Article |
| id | doaj.art-222c05cc9acb48b3a298153d815f8317 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T07:55:50Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-222c05cc9acb48b3a298153d815f83172023-11-22T11:53:07ZengMDPI AGApplied Sciences2076-34172021-09-011118846310.3390/app11188463FTIR Spectroscopy for Identification and Intra-Species Characterization of <i>Serpula lacrymans</i>Rony Barboux0Faisl Bousta1Patrick Di Martino2Laboratoire ERRMECe, CY Cergy Paris University, 95000 Neuville sur Oise, FranceLaboratoire de Recherche des Monuments Historiques, Ministère de la Culture et de la Communication, Centre de Recherche sur la Conservation (CRC-USR 3224), Muséum National d’Histoire Naturelle, CNRS, Sorbonne Universités, 29 Rue de Paris, 77420 Champs-sur-Marne, FranceLaboratoire ERRMECe, CY Cergy Paris University, 95000 Neuville sur Oise, FranceThe dry rot fungus <i>Serpula lacrymans</i> is the most destructive fungal agent of wood building materials in Europe, Russia, North America, and Japan. The identification of this wood-deteriorating agent and the discrimination of different fungal isolates is very important for the control of buildings in general and for the preservation of cultural heritage in particular. The objective of the study was to develop a Fourier transform infrared (FTIR) method coupled with a partial least square discriminant analysis (PLS-DA) for the sample preparation and identification of <i>S. lacrymans</i>. Five distinct <i>S. lacrymans</i> strains were analysed and compared to two strains of unrelated fungal species. Different methods of mycelial growth, sample preparation, and FTIR spectral data normalisation were compared. FTIR analysis of a harvested mycelium grown on the surface of a polyether sulfone microfiltration membrane deposited on a malt extract agar medium, followed by vector normalization and PLS-DA statistical analysis, resulted in 100% correct attribution at phylum, species, and strain level, regardless of the type of standardization used. This study confirms the applicability of FTIR spectroscopy for the identification of <i>S. lacrymans</i> and the discrimination of different strains belonging to this species.https://www.mdpi.com/2076-3417/11/18/8463<i>Serpula lacrymans</i>biodegradationwoodcultural heritageFTIRidentification |
| spellingShingle | Rony Barboux Faisl Bousta Patrick Di Martino FTIR Spectroscopy for Identification and Intra-Species Characterization of <i>Serpula lacrymans</i> Applied Sciences <i>Serpula lacrymans</i> biodegradation wood cultural heritage FTIR identification |
| title | FTIR Spectroscopy for Identification and Intra-Species Characterization of <i>Serpula lacrymans</i> |
| title_full | FTIR Spectroscopy for Identification and Intra-Species Characterization of <i>Serpula lacrymans</i> |
| title_fullStr | FTIR Spectroscopy for Identification and Intra-Species Characterization of <i>Serpula lacrymans</i> |
| title_full_unstemmed | FTIR Spectroscopy for Identification and Intra-Species Characterization of <i>Serpula lacrymans</i> |
| title_short | FTIR Spectroscopy for Identification and Intra-Species Characterization of <i>Serpula lacrymans</i> |
| title_sort | ftir spectroscopy for identification and intra species characterization of i serpula lacrymans i |
| topic | <i>Serpula lacrymans</i> biodegradation wood cultural heritage FTIR identification |
| url | https://www.mdpi.com/2076-3417/11/18/8463 |
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