Cyclic Stretch Force Induces Periodontal Ligament Cells to Secrete Exosomes That Suppress IL-1β Production Through the Inhibition of the NF-κB Signaling Pathway in Macrophages
In the oral mechanical environment, periodontal ligament cells (PDL cells) contribute to maintaining periodontal tissue homeostasis. Recent studies showed that exosomes, which are small vesicles secreted by various types of cells, play a pivotal role in cell-to-cell communication in biological proce...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.01310/full |
| _version_ | 1811277727029788672 |
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| author | Zhuyu Wang Kentarou Maruyama Yukihiko Sakisaka Shigeki Suzuki Hiroyuki Tada Mizuki Suto Masahiro Saito Satoru Yamada Eiji Nemoto |
| author_facet | Zhuyu Wang Kentarou Maruyama Yukihiko Sakisaka Shigeki Suzuki Hiroyuki Tada Mizuki Suto Masahiro Saito Satoru Yamada Eiji Nemoto |
| author_sort | Zhuyu Wang |
| collection | DOAJ |
| description | In the oral mechanical environment, periodontal ligament cells (PDL cells) contribute to maintaining periodontal tissue homeostasis. Recent studies showed that exosomes, which are small vesicles secreted by various types of cells, play a pivotal role in cell-to-cell communication in biological processes. We examined the secretion of exosomes from PDL cells stimulated with cyclic stretch and their role in the inflammatory response of macrophages using the human macrophage cell line THP-1 and human primary monocytes/macrophages. We prepared supernatants from human PDL cells (PDL-sup) stimulated with cyclic stretch. The treatment of macrophages with PDL-sup, but not PDL-sup from unstimulated PDL cells, inhibited the production of IL-1β in LPS/nigericin-stimulated macrophages. The pretreatment of PDL cells with GW4869, an inhibitor of exosome secretion, or siRNA for Rab27B, which controls exosome secretion, abrogated the inhibitory effects of PDL-sup. A transmission electron microscopy analysis demonstrated the existence of exosomes with diameters ranging between 30 and 100 nm in PDL-sup, suggesting that exosomes in PDL-sup contribute to this inhibition. An immunofluorescence microscopy analysis revealed that exosomes labeled with PKH67, a fluorescent dye, were incorporated by macrophages as early as 2 h after the addition of exosomes. Purified exosomes inhibited IL-1β production in LPS/nigericin-stimulated macrophages and the nuclear translocation of NF-κB as well as NF-κB p65 DNA-binding activity in LPS-stimulated macrophages, suggesting that exosomes suppress IL-1β production by inhibiting the NF-κB signaling pathway. Our results indicate that PDL cells in mechanical environments contribute to the maintenance of periodontal immune/inflammatory homeostasis by releasing exosomes. |
| first_indexed | 2024-04-13T00:21:58Z |
| format | Article |
| id | doaj.art-22327a68cb4448509edeeefe1d88cc4c |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-13T00:21:58Z |
| publishDate | 2019-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-22327a68cb4448509edeeefe1d88cc4c2022-12-22T03:10:44ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-06-011010.3389/fimmu.2019.01310442810Cyclic Stretch Force Induces Periodontal Ligament Cells to Secrete Exosomes That Suppress IL-1β Production Through the Inhibition of the NF-κB Signaling Pathway in MacrophagesZhuyu Wang0Kentarou Maruyama1Yukihiko Sakisaka2Shigeki Suzuki3Hiroyuki Tada4Mizuki Suto5Masahiro Saito6Satoru Yamada7Eiji Nemoto8Department of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, JapanDepartment of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, JapanDepartment of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, JapanDepartment of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, JapanDepartment of Oral Immunology, Tohoku University Graduate School of Dentistry, Sendai, JapanDepartment of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, JapanDepartment of Restorative Dentistry, Tohoku University Graduate School of Dentistry, Sendai, JapanDepartment of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, JapanDepartment of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, JapanIn the oral mechanical environment, periodontal ligament cells (PDL cells) contribute to maintaining periodontal tissue homeostasis. Recent studies showed that exosomes, which are small vesicles secreted by various types of cells, play a pivotal role in cell-to-cell communication in biological processes. We examined the secretion of exosomes from PDL cells stimulated with cyclic stretch and their role in the inflammatory response of macrophages using the human macrophage cell line THP-1 and human primary monocytes/macrophages. We prepared supernatants from human PDL cells (PDL-sup) stimulated with cyclic stretch. The treatment of macrophages with PDL-sup, but not PDL-sup from unstimulated PDL cells, inhibited the production of IL-1β in LPS/nigericin-stimulated macrophages. The pretreatment of PDL cells with GW4869, an inhibitor of exosome secretion, or siRNA for Rab27B, which controls exosome secretion, abrogated the inhibitory effects of PDL-sup. A transmission electron microscopy analysis demonstrated the existence of exosomes with diameters ranging between 30 and 100 nm in PDL-sup, suggesting that exosomes in PDL-sup contribute to this inhibition. An immunofluorescence microscopy analysis revealed that exosomes labeled with PKH67, a fluorescent dye, were incorporated by macrophages as early as 2 h after the addition of exosomes. Purified exosomes inhibited IL-1β production in LPS/nigericin-stimulated macrophages and the nuclear translocation of NF-κB as well as NF-κB p65 DNA-binding activity in LPS-stimulated macrophages, suggesting that exosomes suppress IL-1β production by inhibiting the NF-κB signaling pathway. Our results indicate that PDL cells in mechanical environments contribute to the maintenance of periodontal immune/inflammatory homeostasis by releasing exosomes.https://www.frontiersin.org/article/10.3389/fimmu.2019.01310/fullexosomescyclic stretchNF-κB signalinginflammasomeperiodontal ligament cellsmacrophages |
| spellingShingle | Zhuyu Wang Kentarou Maruyama Yukihiko Sakisaka Shigeki Suzuki Hiroyuki Tada Mizuki Suto Masahiro Saito Satoru Yamada Eiji Nemoto Cyclic Stretch Force Induces Periodontal Ligament Cells to Secrete Exosomes That Suppress IL-1β Production Through the Inhibition of the NF-κB Signaling Pathway in Macrophages Frontiers in Immunology exosomes cyclic stretch NF-κB signaling inflammasome periodontal ligament cells macrophages |
| title | Cyclic Stretch Force Induces Periodontal Ligament Cells to Secrete Exosomes That Suppress IL-1β Production Through the Inhibition of the NF-κB Signaling Pathway in Macrophages |
| title_full | Cyclic Stretch Force Induces Periodontal Ligament Cells to Secrete Exosomes That Suppress IL-1β Production Through the Inhibition of the NF-κB Signaling Pathway in Macrophages |
| title_fullStr | Cyclic Stretch Force Induces Periodontal Ligament Cells to Secrete Exosomes That Suppress IL-1β Production Through the Inhibition of the NF-κB Signaling Pathway in Macrophages |
| title_full_unstemmed | Cyclic Stretch Force Induces Periodontal Ligament Cells to Secrete Exosomes That Suppress IL-1β Production Through the Inhibition of the NF-κB Signaling Pathway in Macrophages |
| title_short | Cyclic Stretch Force Induces Periodontal Ligament Cells to Secrete Exosomes That Suppress IL-1β Production Through the Inhibition of the NF-κB Signaling Pathway in Macrophages |
| title_sort | cyclic stretch force induces periodontal ligament cells to secrete exosomes that suppress il 1β production through the inhibition of the nf κb signaling pathway in macrophages |
| topic | exosomes cyclic stretch NF-κB signaling inflammasome periodontal ligament cells macrophages |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.01310/full |
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