Cytotoxic Activity of Fatty Acids From Antarctic Macroalgae on the Growth of Human Breast Cancer Cells
Macroalgae are a natural source of clinically relevant molecules such as polyunsaturated and monounsaturated fatty acids. The Antarctic environment, due to its cold climate, leads to high production of these bioactive molecules. Adenocystis utricularis, Curdiea racovitzae, and Georgiella confluens f...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2018.00185/full |
| _version_ | 1818143146704371712 |
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| author | Bruna Silveira Pacheco Bruna Silveira Pacheco Marco Aurélio Ziemann dos Santos Eduarda Schultze Rosiane Mastelari Martins Rafael Guerra Lund Fabiana Kömmling Seixas Pio Colepicolo Tiago Collares Tiago Collares Favero Reisdorfer Paula Claudio Martin Pereira De Pereira Claudio Martin Pereira De Pereira |
| author_facet | Bruna Silveira Pacheco Bruna Silveira Pacheco Marco Aurélio Ziemann dos Santos Eduarda Schultze Rosiane Mastelari Martins Rafael Guerra Lund Fabiana Kömmling Seixas Pio Colepicolo Tiago Collares Tiago Collares Favero Reisdorfer Paula Claudio Martin Pereira De Pereira Claudio Martin Pereira De Pereira |
| author_sort | Bruna Silveira Pacheco |
| collection | DOAJ |
| description | Macroalgae are a natural source of clinically relevant molecules such as polyunsaturated and monounsaturated fatty acids. The Antarctic environment, due to its cold climate, leads to high production of these bioactive molecules. Adenocystis utricularis, Curdiea racovitzae, and Georgiella confluens from three distinct islands in the Antarctic Peninsula were collected and analyzed for their fatty acid content by gas chromatography flame ionization detection. Results revealed that the algal extracts consisted of 22 fatty acids, of which 9 were saturated, 4 were monounsaturated, and 9 were polyunsaturated (PUFA). In addition, fucosterol was identified within the lipidic extracts. The cytotoxic activity of these fatty acids was evaluated in human breast cancer cell lines MCF-7 and MDA-MB-231. The most notable result was the effect of PUFA on the growth inhibition of cancer cells ranging from 61.04 to 69.78% in comparison to control cells. Significant cytotoxic activity of fatty acids from A. utricularis was observed at 48 h, resulting in an inhibition of growth of more than 50% for breast cancer cells at a concentration of 100 μg/mL. A cell viability assay showed that the fatty acids from A. utricularis significantly reduced cell viability (68.7% in MCF-7 and 89% in MDA-MB-231 after 72 h of exposure). At the same time, DAPI staining demonstrated chromatin condensation, and apoptotic bodies formed in cells that were cultured with fatty acids from A. utricularis. These data indicate that fatty acids from Antarctic macroalgae have the potential to reduce the proliferation of and induce apoptosis in breast cancer cells. |
| first_indexed | 2024-12-11T11:27:02Z |
| format | Article |
| id | doaj.art-2234f301e72441a98783e87dbb8935dd |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-11T11:27:02Z |
| publishDate | 2018-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-2234f301e72441a98783e87dbb8935dd2022-12-22T01:08:59ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852018-12-01610.3389/fbioe.2018.00185398299Cytotoxic Activity of Fatty Acids From Antarctic Macroalgae on the Growth of Human Breast Cancer CellsBruna Silveira Pacheco0Bruna Silveira Pacheco1Marco Aurélio Ziemann dos Santos2Eduarda Schultze3Rosiane Mastelari Martins4Rafael Guerra Lund5Fabiana Kömmling Seixas6Pio Colepicolo7Tiago Collares8Tiago Collares9Favero Reisdorfer Paula10Claudio Martin Pereira De Pereira11Claudio Martin Pereira De Pereira12Bioforensic Research Group, Lipidomic and Bio-Organic Laboratory, Postgraduate Program in Biochemistry and Bioprospection, Federal University of Pelotas, Pelotas, BrazilResearch Group on Cellular and Molecular Oncology, Postgraduate Program in Biotechnology, Federal University of Pelotas, Pelotas, BrazilBioforensic Research Group, Lipidomic and Bio-Organic Laboratory, Postgraduate Program in Biochemistry and Bioprospection, Federal University of Pelotas, Pelotas, BrazilResearch Group on Cellular and Molecular Oncology, Postgraduate Program in Biotechnology, Federal University of Pelotas, Pelotas, BrazilBioforensic Research Group, Lipidomic and Bio-Organic Laboratory, Postgraduate Program in Biochemistry and Bioprospection, Federal University of Pelotas, Pelotas, BrazilBioforensic Research Group, Lipidomic and Bio-Organic Laboratory, Postgraduate Program in Biochemistry and Bioprospection, Federal University of Pelotas, Pelotas, BrazilResearch Group on Cellular and Molecular Oncology, Postgraduate Program in Biotechnology, Federal University of Pelotas, Pelotas, BrazilChemistry Institute, University of São Paulo, São Paulo, BrazilBioforensic Research Group, Lipidomic and Bio-Organic Laboratory, Postgraduate Program in Biochemistry and Bioprospection, Federal University of Pelotas, Pelotas, BrazilResearch Group on Cellular and Molecular Oncology, Postgraduate Program in Biotechnology, Federal University of Pelotas, Pelotas, BrazilLaboratory of Research and Drugs Development, Pharmaceutical Sciences Postgraduate Program, Federal University of Pampa, Bagé, BrazilBioforensic Research Group, Lipidomic and Bio-Organic Laboratory, Postgraduate Program in Biochemistry and Bioprospection, Federal University of Pelotas, Pelotas, BrazilResearch Group on Cellular and Molecular Oncology, Postgraduate Program in Biotechnology, Federal University of Pelotas, Pelotas, BrazilMacroalgae are a natural source of clinically relevant molecules such as polyunsaturated and monounsaturated fatty acids. The Antarctic environment, due to its cold climate, leads to high production of these bioactive molecules. Adenocystis utricularis, Curdiea racovitzae, and Georgiella confluens from three distinct islands in the Antarctic Peninsula were collected and analyzed for their fatty acid content by gas chromatography flame ionization detection. Results revealed that the algal extracts consisted of 22 fatty acids, of which 9 were saturated, 4 were monounsaturated, and 9 were polyunsaturated (PUFA). In addition, fucosterol was identified within the lipidic extracts. The cytotoxic activity of these fatty acids was evaluated in human breast cancer cell lines MCF-7 and MDA-MB-231. The most notable result was the effect of PUFA on the growth inhibition of cancer cells ranging from 61.04 to 69.78% in comparison to control cells. Significant cytotoxic activity of fatty acids from A. utricularis was observed at 48 h, resulting in an inhibition of growth of more than 50% for breast cancer cells at a concentration of 100 μg/mL. A cell viability assay showed that the fatty acids from A. utricularis significantly reduced cell viability (68.7% in MCF-7 and 89% in MDA-MB-231 after 72 h of exposure). At the same time, DAPI staining demonstrated chromatin condensation, and apoptotic bodies formed in cells that were cultured with fatty acids from A. utricularis. These data indicate that fatty acids from Antarctic macroalgae have the potential to reduce the proliferation of and induce apoptosis in breast cancer cells.https://www.frontiersin.org/article/10.3389/fbioe.2018.00185/fullSouth Shetland IslandsAntarcticmacroalgaefatty acidsteroidpolyunsaturated fatty acids |
| spellingShingle | Bruna Silveira Pacheco Bruna Silveira Pacheco Marco Aurélio Ziemann dos Santos Eduarda Schultze Rosiane Mastelari Martins Rafael Guerra Lund Fabiana Kömmling Seixas Pio Colepicolo Tiago Collares Tiago Collares Favero Reisdorfer Paula Claudio Martin Pereira De Pereira Claudio Martin Pereira De Pereira Cytotoxic Activity of Fatty Acids From Antarctic Macroalgae on the Growth of Human Breast Cancer Cells Frontiers in Bioengineering and Biotechnology South Shetland Islands Antarctic macroalgae fatty acid steroid polyunsaturated fatty acids |
| title | Cytotoxic Activity of Fatty Acids From Antarctic Macroalgae on the Growth of Human Breast Cancer Cells |
| title_full | Cytotoxic Activity of Fatty Acids From Antarctic Macroalgae on the Growth of Human Breast Cancer Cells |
| title_fullStr | Cytotoxic Activity of Fatty Acids From Antarctic Macroalgae on the Growth of Human Breast Cancer Cells |
| title_full_unstemmed | Cytotoxic Activity of Fatty Acids From Antarctic Macroalgae on the Growth of Human Breast Cancer Cells |
| title_short | Cytotoxic Activity of Fatty Acids From Antarctic Macroalgae on the Growth of Human Breast Cancer Cells |
| title_sort | cytotoxic activity of fatty acids from antarctic macroalgae on the growth of human breast cancer cells |
| topic | South Shetland Islands Antarctic macroalgae fatty acid steroid polyunsaturated fatty acids |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2018.00185/full |
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