Application of droplet digital PCR for the detection of vector copy number in clinical CAR/TCR T cell products

Application of droplet digital PCR for the detection of vector copy number in clinical CAR/TCR T cell products

Abstract Background Genetically engineered T cells have become an important therapy for B-cell malignancies. Measuring the efficiency of vector integration into the T cell genome is important for assessing the potency and safety of these cancer immunotherapies. Methods A digital droplet polymerase c...

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Bibliographic Details
Main Authors: Alex Lu, Hui Liu, Rongye Shi, Yihua Cai, Jinxia Ma, Lipei Shao, Victor Rong, Nikolaos Gkitsas, Hong Lei, Steven L. Highfill, Sandhya Panch, David F. Stroncek, Ping Jin
Format: Article
Language:English
Published: BMC 2020-05-01
Series:Journal of Translational Medicine
Subjects:
Droplet digital PCR
Vector copy number
Genetically engineered T cells
Gene therapy
Chimeric antigen receptor (CAR) T cells
T Cell Receptor (TCR)-engineered T cells
Online Access:http://link.springer.com/article/10.1186/s12967-020-02358-0

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