Combined analysis of mRNA expression of ERCC1, BAG-1, BRCA1, RRM1 and TUBB3 to predict prognosis in patients with non-small cell lung cancer who received adjuvant chemotherapy
<p>Abstract</p> <p>Background</p> <p>The aim of this study was to investigate prognostic value of excision repair cross-complementing 1 (ERCC1), BCL2-associated athanogene (BAG-1), the breast and ovarian cancer susceptibility gene 1 (BRCA1), ribonucleotide reductase sub...
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| Format: | Article |
| Language: | English |
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BMC
2012-03-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Subjects: | |
| Online Access: | http://www.jeccr.com/content/31/1/25 |
| _version_ | 1818084205173669888 |
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| author | Leng Xue-Feng Chen Ming-Wu Xian Lei Dai Lei Ma Guang-Yao Li Man-Hong |
| author_facet | Leng Xue-Feng Chen Ming-Wu Xian Lei Dai Lei Ma Guang-Yao Li Man-Hong |
| author_sort | Leng Xue-Feng |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The aim of this study was to investigate prognostic value of excision repair cross-complementing 1 (ERCC1), BCL2-associated athanogene (BAG-1), the breast and ovarian cancer susceptibility gene 1 (BRCA1), ribonucleotide reductase subunit M1 (RRM1) and class III β-tubulin (TUBB3) in patients with non-small cell lung cancer (NSCLC) who received platinum- based adjuvant chemotherapy.</p> <p>Methods</p> <p>Messenger RNA expressions of these genes were examined in 85 tumor tissues and 34 adjacent tissue samples using semi-quantitative RT-PCR. The expressions of these five genes were analyzed in relation to chemotherapy and progression-free survival (PFS) and overall survival (OS). Seventy-four patients were enrolled into chemotherapy.</p> <p>Results</p> <p>Patients with ERCC1 or BAG-1 negative expression had a significantly longer PFS (<it>P </it>= 0.001 and <it>P </it>= 0.001) and OS (<it>P </it>= 0.001 and <it>P </it>= 0.001) than those with positive expression. Patients with negative ERCC1 and BAG-1 expression benefited more from platinum regimen (<it>P </it>= 0.001 and <it>P </it>= 0.002). Patients with BRCA1 negative expression might have a longer OS (<it>P </it>= 0.052), but not PFS (<it>P </it>= 0.088) than those with BRCA1 positive expression. A significant relationship was observed between the mRNA expression of ERCC1 and BAG-1 (<it>P </it>= 0.042). In multivariate analysis, ERCC1 and BAG-1 were significantly favorable factors for PFS (<it>P </it>= 0.018 and <it>P </it>= 0.017) and OS (<it>P </it>= 0.027 and <it>P </it>= 0.022).</p> <p>Conclusions</p> <p>ERCC1 and BAG-1 are determinants of survival after surgical treatment of NSCLC, and its mRNA expression in tumor tissues could be used to predict the prognosis of NSCLC treated by platinum.</p> |
| first_indexed | 2024-12-10T19:50:11Z |
| format | Article |
| id | doaj.art-2237b0a638a1492c926abcef89582815 |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-12-10T19:50:11Z |
| publishDate | 2012-03-01 |
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| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-2237b0a638a1492c926abcef895828152022-12-22T01:35:48ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662012-03-013112510.1186/1756-9966-31-25Combined analysis of mRNA expression of ERCC1, BAG-1, BRCA1, RRM1 and TUBB3 to predict prognosis in patients with non-small cell lung cancer who received adjuvant chemotherapyLeng Xue-FengChen Ming-WuXian LeiDai LeiMa Guang-YaoLi Man-Hong<p>Abstract</p> <p>Background</p> <p>The aim of this study was to investigate prognostic value of excision repair cross-complementing 1 (ERCC1), BCL2-associated athanogene (BAG-1), the breast and ovarian cancer susceptibility gene 1 (BRCA1), ribonucleotide reductase subunit M1 (RRM1) and class III β-tubulin (TUBB3) in patients with non-small cell lung cancer (NSCLC) who received platinum- based adjuvant chemotherapy.</p> <p>Methods</p> <p>Messenger RNA expressions of these genes were examined in 85 tumor tissues and 34 adjacent tissue samples using semi-quantitative RT-PCR. The expressions of these five genes were analyzed in relation to chemotherapy and progression-free survival (PFS) and overall survival (OS). Seventy-four patients were enrolled into chemotherapy.</p> <p>Results</p> <p>Patients with ERCC1 or BAG-1 negative expression had a significantly longer PFS (<it>P </it>= 0.001 and <it>P </it>= 0.001) and OS (<it>P </it>= 0.001 and <it>P </it>= 0.001) than those with positive expression. Patients with negative ERCC1 and BAG-1 expression benefited more from platinum regimen (<it>P </it>= 0.001 and <it>P </it>= 0.002). Patients with BRCA1 negative expression might have a longer OS (<it>P </it>= 0.052), but not PFS (<it>P </it>= 0.088) than those with BRCA1 positive expression. A significant relationship was observed between the mRNA expression of ERCC1 and BAG-1 (<it>P </it>= 0.042). In multivariate analysis, ERCC1 and BAG-1 were significantly favorable factors for PFS (<it>P </it>= 0.018 and <it>P </it>= 0.017) and OS (<it>P </it>= 0.027 and <it>P </it>= 0.022).</p> <p>Conclusions</p> <p>ERCC1 and BAG-1 are determinants of survival after surgical treatment of NSCLC, and its mRNA expression in tumor tissues could be used to predict the prognosis of NSCLC treated by platinum.</p>http://www.jeccr.com/content/31/1/25Non-small cell lung cancerERCC1BAG-1BRCA1RRM1TUBB3 |
| spellingShingle | Leng Xue-Feng Chen Ming-Wu Xian Lei Dai Lei Ma Guang-Yao Li Man-Hong Combined analysis of mRNA expression of ERCC1, BAG-1, BRCA1, RRM1 and TUBB3 to predict prognosis in patients with non-small cell lung cancer who received adjuvant chemotherapy Journal of Experimental & Clinical Cancer Research Non-small cell lung cancer ERCC1 BAG-1 BRCA1 RRM1 TUBB3 |
| title | Combined analysis of mRNA expression of ERCC1, BAG-1, BRCA1, RRM1 and TUBB3 to predict prognosis in patients with non-small cell lung cancer who received adjuvant chemotherapy |
| title_full | Combined analysis of mRNA expression of ERCC1, BAG-1, BRCA1, RRM1 and TUBB3 to predict prognosis in patients with non-small cell lung cancer who received adjuvant chemotherapy |
| title_fullStr | Combined analysis of mRNA expression of ERCC1, BAG-1, BRCA1, RRM1 and TUBB3 to predict prognosis in patients with non-small cell lung cancer who received adjuvant chemotherapy |
| title_full_unstemmed | Combined analysis of mRNA expression of ERCC1, BAG-1, BRCA1, RRM1 and TUBB3 to predict prognosis in patients with non-small cell lung cancer who received adjuvant chemotherapy |
| title_short | Combined analysis of mRNA expression of ERCC1, BAG-1, BRCA1, RRM1 and TUBB3 to predict prognosis in patients with non-small cell lung cancer who received adjuvant chemotherapy |
| title_sort | combined analysis of mrna expression of ercc1 bag 1 brca1 rrm1 and tubb3 to predict prognosis in patients with non small cell lung cancer who received adjuvant chemotherapy |
| topic | Non-small cell lung cancer ERCC1 BAG-1 BRCA1 RRM1 TUBB3 |
| url | http://www.jeccr.com/content/31/1/25 |
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