Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated blaIMI-6 Gene
ABSTRACT We report a plasmid-encoded IMI-6 carbapenemase in a clinical isolate of Enterobacter ludwigii from Spain. The isolate belongs to ST641 and was susceptible to expanded-spectrum cephalosporins and resistant to carbapenems. The modified carbapenem inactivation method (mCIM) test was positive,...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-06-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.04620-22 |
| _version_ | 1797803445554511872 |
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| author | T. Blanco-Martín J. Guzmán-Puche C. Riazzo M. Gasca-Santiyán M. Hernández-García R. Cantón J. Torre-Cisneros C. Herrera L. Martínez-Martínez |
| author_facet | T. Blanco-Martín J. Guzmán-Puche C. Riazzo M. Gasca-Santiyán M. Hernández-García R. Cantón J. Torre-Cisneros C. Herrera L. Martínez-Martínez |
| author_sort | T. Blanco-Martín |
| collection | DOAJ |
| description | ABSTRACT We report a plasmid-encoded IMI-6 carbapenemase in a clinical isolate of Enterobacter ludwigii from Spain. The isolate belongs to ST641 and was susceptible to expanded-spectrum cephalosporins and resistant to carbapenems. The modified carbapenem inactivation method (mCIM) test was positive, but β-Carba was negative. Whole-genome sequencing identified the blaIMI-6 gene located in a conjugative IncFIIY plasmid and associated with the LysR-like regulator imiR. Both genes were bracketed by an ISEclI-like insertion sequence and a putatively defective ISEc36 insertion sequence. IMPORTANCE IMI carbapenemases confer an unusual resistance pattern of susceptibility to broad-spectrum cephalosporins and piperacillin-tazobactam but decreased susceptibility to carbapenems, which may make them difficult to detect in routine practice. Commercially available molecular methods for the detection of carbapenemases in clinical laboratories do not usually include blaIMI genes, which could contribute to the hidden dissemination of bacteria producing these enzymes. Techniques should be implemented to detect minor carbapenemases that are not very frequent in our environment and control their dissemination. |
| first_indexed | 2024-03-13T05:20:57Z |
| format | Article |
| id | doaj.art-223bab20449b4eeab3937016741198d4 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-13T05:20:57Z |
| publishDate | 2023-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-223bab20449b4eeab3937016741198d42023-06-15T13:18:30ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-06-0111310.1128/spectrum.04620-22Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated blaIMI-6 GeneT. Blanco-Martín0J. Guzmán-Puche1C. Riazzo2M. Gasca-Santiyán3M. Hernández-García4R. Cantón5J. Torre-Cisneros6C. Herrera7L. Martínez-Martínez8Microbiology Unit, Reina Sofía University Hospital, Cordoba, SpainMicrobiology Unit, Reina Sofía University Hospital, Cordoba, SpainMicrobiology Unit, Reina Sofía University Hospital, Cordoba, SpainMicrobiology Unit, Reina Sofía University Hospital, Cordoba, SpainCIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, SpainCIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, Madrid, SpainMaimonides Biomedical Research Institute of Cordoba (IMIBIC), Cordoba, SpainMaimonides Biomedical Research Institute of Cordoba (IMIBIC), Cordoba, SpainMicrobiology Unit, Reina Sofía University Hospital, Cordoba, SpainABSTRACT We report a plasmid-encoded IMI-6 carbapenemase in a clinical isolate of Enterobacter ludwigii from Spain. The isolate belongs to ST641 and was susceptible to expanded-spectrum cephalosporins and resistant to carbapenems. The modified carbapenem inactivation method (mCIM) test was positive, but β-Carba was negative. Whole-genome sequencing identified the blaIMI-6 gene located in a conjugative IncFIIY plasmid and associated with the LysR-like regulator imiR. Both genes were bracketed by an ISEclI-like insertion sequence and a putatively defective ISEc36 insertion sequence. IMPORTANCE IMI carbapenemases confer an unusual resistance pattern of susceptibility to broad-spectrum cephalosporins and piperacillin-tazobactam but decreased susceptibility to carbapenems, which may make them difficult to detect in routine practice. Commercially available molecular methods for the detection of carbapenemases in clinical laboratories do not usually include blaIMI genes, which could contribute to the hidden dissemination of bacteria producing these enzymes. Techniques should be implemented to detect minor carbapenemases that are not very frequent in our environment and control their dissemination.https://journals.asm.org/doi/10.1128/spectrum.04620-22Enterobacter ludwigiiIMI-6carbapenemase |
| spellingShingle | T. Blanco-Martín J. Guzmán-Puche C. Riazzo M. Gasca-Santiyán M. Hernández-García R. Cantón J. Torre-Cisneros C. Herrera L. Martínez-Martínez Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated blaIMI-6 Gene Microbiology Spectrum Enterobacter ludwigii IMI-6 carbapenemase |
| title | Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated blaIMI-6 Gene |
| title_full | Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated blaIMI-6 Gene |
| title_fullStr | Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated blaIMI-6 Gene |
| title_full_unstemmed | Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated blaIMI-6 Gene |
| title_short | Phenotypic and Molecular Characterization of an Enterobacter ludwigii Clinical Isolate Carrying a Plasmid-Mediated blaIMI-6 Gene |
| title_sort | phenotypic and molecular characterization of an enterobacter ludwigii clinical isolate carrying a plasmid mediated blaimi 6 gene |
| topic | Enterobacter ludwigii IMI-6 carbapenemase |
| url | https://journals.asm.org/doi/10.1128/spectrum.04620-22 |
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