Aged black tea alleviates constipation in mice by modulating intestinal neurotransmitters and decreasing AQP3 and AQP9 expression
Background: Black tea is fully fermented tea with abundant functional components that benefit the gastrointestinal tract. But whether black tea extract relieves constipation is unknown. Therefore, we used loperamide to induce constipation in mice to assess the therapeutical effect of extracts from a...
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| Format: | Article |
| Language: | English |
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Swedish Nutrition Foundation
2023-10-01
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| Series: | Food & Nutrition Research |
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| Online Access: | https://foodandnutritionresearch.net/index.php/fnr/article/view/9513/16346 |
| _version_ | 1797643379097468928 |
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| author | Yu Wu Qiuhua Li Junxi Cao Fenling Fan Lishe Gan Rihui Wu Jingwei Jin Ruohong Chen Lingli Sun Zhenbiao Zhang Xingfei Lai Wing-Leung Wong Shili Sun Dongli Li |
| author_facet | Yu Wu Qiuhua Li Junxi Cao Fenling Fan Lishe Gan Rihui Wu Jingwei Jin Ruohong Chen Lingli Sun Zhenbiao Zhang Xingfei Lai Wing-Leung Wong Shili Sun Dongli Li |
| author_sort | Yu Wu |
| collection | DOAJ |
| description | Background: Black tea is fully fermented tea with abundant functional components that benefit the gastrointestinal tract. But whether black tea extract relieves constipation is unknown. Therefore, we used loperamide to induce constipation in mice to assess the therapeutical effect of extracts from aged black tea with different storage times. Design: Sixty-three C57BL/6J male mice were randomly divided into Control group (Con), Model group (Mod), Positive group (Pos), aged 6 years group (15Y), aged 4 years group (17Y), aged 2 years group (19Y), and unaged group (21Y). Mice were given loperamide (20 mg/kg, twice a day) to induce constipation for 10 days, and black tea extracts (500 mg/kg) were intragastrically given for 7 days while continuing modeling. Results: The results showed that black tea extracts relieved constipation symptoms by improving defecation weight, fecal water content, and gastrointestinal transit rate. Black tea extracts can also protect colon tissue, regulate serum neurotransmitters, increase the levels of excitatory neurotransmitters motilin (MTL) and substance P (SP), and decrease the levels of inhibitory neurotransmitters vasoactive intestinal peptide (VIP) and nitric oxide (NO). Immunohistochemistry (IHC) showed that black tea extracts were able to reduce AQP3 and AQP9 expression in the colon of constipated mice. In addition, Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) showed that black tea extracts could decrease AQP3 and AQP9 mRNA expression. The relief effect of aged black tea (15Y) with the longest storage was better than that of other years, which may be due to the role of active ingredients such as thearubigins (TRs), soluble sugar, tea polysaccharide (TPS), gallic acid (GA), and catechin gallate (CG) in aged black tea. Conclusions: Based on these results, we believe that regular consumption of black tea is effective in relieving constipation, and that black tea is more effective in relieving constipation as the storage time increases. |
| first_indexed | 2024-03-11T14:13:55Z |
| format | Article |
| id | doaj.art-223fdd1e7f054400a88225df7243ecca |
| institution | Directory Open Access Journal |
| issn | 1654-661X |
| language | English |
| last_indexed | 2024-03-11T14:13:55Z |
| publishDate | 2023-10-01 |
| publisher | Swedish Nutrition Foundation |
| record_format | Article |
| series | Food & Nutrition Research |
| spelling | doaj.art-223fdd1e7f054400a88225df7243ecca2023-11-01T12:08:20ZengSwedish Nutrition FoundationFood & Nutrition Research1654-661X2023-10-016711210.29219/fnr.v67.95139513Aged black tea alleviates constipation in mice by modulating intestinal neurotransmitters and decreasing AQP3 and AQP9 expressionYu Wu0Qiuhua Li1Junxi Cao2Fenling Fan3Lishe Gan4Rihui Wu5Jingwei Jin6Ruohong Chen7Lingli Sun8Zhenbiao Zhang9Xingfei Lai10Wing-Leung Wong11Shili Sun12Dongli Li13School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, ChinaTea Research Institute, Guangdong Academy of Agricultural Sciences / Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, ChinaTea Research Institute, Guangdong Academy of Agricultural Sciences / Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen, ChinaTea Research Institute, Guangdong Academy of Agricultural Sciences / Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, ChinaTea Research Institute, Guangdong Academy of Agricultural Sciences / Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, ChinaTea Research Institute, Guangdong Academy of Agricultural Sciences / Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, ChinaTea Research Institute, Guangdong Academy of Agricultural Sciences / Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen, ChinaTea Research Institute, Guangdong Academy of Agricultural Sciences / Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen, ChinaBackground: Black tea is fully fermented tea with abundant functional components that benefit the gastrointestinal tract. But whether black tea extract relieves constipation is unknown. Therefore, we used loperamide to induce constipation in mice to assess the therapeutical effect of extracts from aged black tea with different storage times. Design: Sixty-three C57BL/6J male mice were randomly divided into Control group (Con), Model group (Mod), Positive group (Pos), aged 6 years group (15Y), aged 4 years group (17Y), aged 2 years group (19Y), and unaged group (21Y). Mice were given loperamide (20 mg/kg, twice a day) to induce constipation for 10 days, and black tea extracts (500 mg/kg) were intragastrically given for 7 days while continuing modeling. Results: The results showed that black tea extracts relieved constipation symptoms by improving defecation weight, fecal water content, and gastrointestinal transit rate. Black tea extracts can also protect colon tissue, regulate serum neurotransmitters, increase the levels of excitatory neurotransmitters motilin (MTL) and substance P (SP), and decrease the levels of inhibitory neurotransmitters vasoactive intestinal peptide (VIP) and nitric oxide (NO). Immunohistochemistry (IHC) showed that black tea extracts were able to reduce AQP3 and AQP9 expression in the colon of constipated mice. In addition, Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) showed that black tea extracts could decrease AQP3 and AQP9 mRNA expression. The relief effect of aged black tea (15Y) with the longest storage was better than that of other years, which may be due to the role of active ingredients such as thearubigins (TRs), soluble sugar, tea polysaccharide (TPS), gallic acid (GA), and catechin gallate (CG) in aged black tea. Conclusions: Based on these results, we believe that regular consumption of black tea is effective in relieving constipation, and that black tea is more effective in relieving constipation as the storage time increases.https://foodandnutritionresearch.net/index.php/fnr/article/view/9513/16346aged black teaconstipationintestinal neurotransmitteraqp3aqp9 |
| spellingShingle | Yu Wu Qiuhua Li Junxi Cao Fenling Fan Lishe Gan Rihui Wu Jingwei Jin Ruohong Chen Lingli Sun Zhenbiao Zhang Xingfei Lai Wing-Leung Wong Shili Sun Dongli Li Aged black tea alleviates constipation in mice by modulating intestinal neurotransmitters and decreasing AQP3 and AQP9 expression Food & Nutrition Research aged black tea constipation intestinal neurotransmitter aqp3 aqp9 |
| title | Aged black tea alleviates constipation in mice by modulating intestinal neurotransmitters and decreasing AQP3 and AQP9 expression |
| title_full | Aged black tea alleviates constipation in mice by modulating intestinal neurotransmitters and decreasing AQP3 and AQP9 expression |
| title_fullStr | Aged black tea alleviates constipation in mice by modulating intestinal neurotransmitters and decreasing AQP3 and AQP9 expression |
| title_full_unstemmed | Aged black tea alleviates constipation in mice by modulating intestinal neurotransmitters and decreasing AQP3 and AQP9 expression |
| title_short | Aged black tea alleviates constipation in mice by modulating intestinal neurotransmitters and decreasing AQP3 and AQP9 expression |
| title_sort | aged black tea alleviates constipation in mice by modulating intestinal neurotransmitters and decreasing aqp3 and aqp9 expression |
| topic | aged black tea constipation intestinal neurotransmitter aqp3 aqp9 |
| url | https://foodandnutritionresearch.net/index.php/fnr/article/view/9513/16346 |
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