How fall dormancy benefits alfalfa winter-survival? Physiologic and transcriptomic analyses of dormancy process
Abstract Background Fall dormancy and freezing tolerance characterized as two important phenotypic traits, have great effects on productivity and persistence of alfalfa (Medicago sativa L.). Despite the fact that one of the most limiting traits for alfalfa freezing tolerance in winter is fall dorman...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1773-3 |
| _version_ | 1818970923828510720 |
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| author | Zhi-Ying Liu Taogetao Baoyin Xi-Liang Li Zong-Li Wang |
| author_facet | Zhi-Ying Liu Taogetao Baoyin Xi-Liang Li Zong-Li Wang |
| author_sort | Zhi-Ying Liu |
| collection | DOAJ |
| description | Abstract Background Fall dormancy and freezing tolerance characterized as two important phenotypic traits, have great effects on productivity and persistence of alfalfa (Medicago sativa L.). Despite the fact that one of the most limiting traits for alfalfa freezing tolerance in winter is fall dormancy, the interplay between fall dormancy and cold acclimation processes of alfalfa remains largely unknown. We compared the plant regrowth, winter survival, raffinose and amino acids accumulation, and genome-wide differentially expressed genes of fall-dormant cultivar with non-dormant cultivar under cold acclimation. Results Averaged over both years, the non-dormant alfalfa exhibited largely rapid regrowth compared with fall dormant alfalfa after last cutting in autumn, but the winter survival rate of fall dormant alfalfa was about 34-fold higher than that of non-dormant alfalfa. The accumulation of raffinose and amino acids were significantly increased in fall dormant alfalfa, whereas were decreased in non-dormant alfalfa under cold acclimation. Expressions of candidate genes encoding raffinose biosynthesis genes were highly up-regulated in fall dormant alfalfa, but down-regulated in non-dormant alfalfa under cold acclimation. In fall dormant alfalfa, there was a significantly down-regulated expression of candidate genes encoding the glutamine synthase, which is indirectly involved in the proline metabolism. A total of eight significantly differentially expressed transcription factors (TFs) related to CBF and ABRE-BFs were identified. The most up-regulated TFs in fall dormant alfalfa cultivar were ABF4 and DREB1C. Conclusions Fall dormant alfalfa drastically increased raffinose and amino acids accumulation under cold acclimation. Raffinose-associated and amino acid-associated genes involved in metabolic pathways were more highly expressed in fall dormant alfalfa than non-dormant alfalfa under cold acclimation. This global survey of transcriptome profiles provides new insights into the interplay between fall dormancy and cold acclimation in alfalfa. |
| first_indexed | 2024-12-20T14:44:12Z |
| format | Article |
| id | doaj.art-22401e4a45ac4a40b590071c7140a3b7 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-20T14:44:12Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-22401e4a45ac4a40b590071c7140a3b72022-12-21T19:37:11ZengBMCBMC Plant Biology1471-22292019-05-0119111310.1186/s12870-019-1773-3How fall dormancy benefits alfalfa winter-survival? Physiologic and transcriptomic analyses of dormancy processZhi-Ying Liu0Taogetao Baoyin1Xi-Liang Li2Zong-Li Wang3Key Laboratory of Grassland Ecology, School of Ecology and Environment, Inner Mongolia UniversityKey Laboratory of Grassland Ecology, School of Ecology and Environment, Inner Mongolia UniversityKey Laboratory of Grassland Ecology and Restoration of Ministry of Agriculture, National Forage Improvement Center, Institute of Grassland Research, Chinese Academy of Agricultural SciencesChina Animal Health and Epidemiology CenterAbstract Background Fall dormancy and freezing tolerance characterized as two important phenotypic traits, have great effects on productivity and persistence of alfalfa (Medicago sativa L.). Despite the fact that one of the most limiting traits for alfalfa freezing tolerance in winter is fall dormancy, the interplay between fall dormancy and cold acclimation processes of alfalfa remains largely unknown. We compared the plant regrowth, winter survival, raffinose and amino acids accumulation, and genome-wide differentially expressed genes of fall-dormant cultivar with non-dormant cultivar under cold acclimation. Results Averaged over both years, the non-dormant alfalfa exhibited largely rapid regrowth compared with fall dormant alfalfa after last cutting in autumn, but the winter survival rate of fall dormant alfalfa was about 34-fold higher than that of non-dormant alfalfa. The accumulation of raffinose and amino acids were significantly increased in fall dormant alfalfa, whereas were decreased in non-dormant alfalfa under cold acclimation. Expressions of candidate genes encoding raffinose biosynthesis genes were highly up-regulated in fall dormant alfalfa, but down-regulated in non-dormant alfalfa under cold acclimation. In fall dormant alfalfa, there was a significantly down-regulated expression of candidate genes encoding the glutamine synthase, which is indirectly involved in the proline metabolism. A total of eight significantly differentially expressed transcription factors (TFs) related to CBF and ABRE-BFs were identified. The most up-regulated TFs in fall dormant alfalfa cultivar were ABF4 and DREB1C. Conclusions Fall dormant alfalfa drastically increased raffinose and amino acids accumulation under cold acclimation. Raffinose-associated and amino acid-associated genes involved in metabolic pathways were more highly expressed in fall dormant alfalfa than non-dormant alfalfa under cold acclimation. This global survey of transcriptome profiles provides new insights into the interplay between fall dormancy and cold acclimation in alfalfa.http://link.springer.com/article/10.1186/s12870-019-1773-3Medicago sativaFall dormancyRNA-SeqqRT-PCRRaffinose and amino acids |
| spellingShingle | Zhi-Ying Liu Taogetao Baoyin Xi-Liang Li Zong-Li Wang How fall dormancy benefits alfalfa winter-survival? Physiologic and transcriptomic analyses of dormancy process BMC Plant Biology Medicago sativa Fall dormancy RNA-Seq qRT-PCR Raffinose and amino acids |
| title | How fall dormancy benefits alfalfa winter-survival? Physiologic and transcriptomic analyses of dormancy process |
| title_full | How fall dormancy benefits alfalfa winter-survival? Physiologic and transcriptomic analyses of dormancy process |
| title_fullStr | How fall dormancy benefits alfalfa winter-survival? Physiologic and transcriptomic analyses of dormancy process |
| title_full_unstemmed | How fall dormancy benefits alfalfa winter-survival? Physiologic and transcriptomic analyses of dormancy process |
| title_short | How fall dormancy benefits alfalfa winter-survival? Physiologic and transcriptomic analyses of dormancy process |
| title_sort | how fall dormancy benefits alfalfa winter survival physiologic and transcriptomic analyses of dormancy process |
| topic | Medicago sativa Fall dormancy RNA-Seq qRT-PCR Raffinose and amino acids |
| url | http://link.springer.com/article/10.1186/s12870-019-1773-3 |
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