Polyphenolic Extract from <i>Sambucus ebulus</i> L. Leaves Free and Loaded into Lipid Vesicles
The paper deals with the preparation and characterisation of hydroalcoholic polyphenolic extract from <i>Sambucus ebulus</i> (SE) leaves that was further loaded into three-types of lipid vesicles: liposomes, transfersomes, and ethosomes, to improve its bioavailability and achieve an opti...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-12-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/1/56 |
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| author | Ramona-Daniela Păvăloiu Fawzia Sha’at Corina Bubueanu Mihaela Deaconu Georgeta Neagu Mousa Sha’at Mihai Anastasescu Mona Mihailescu Cristian Matei Gheorghe Nechifor Daniela Berger |
| author_facet | Ramona-Daniela Păvăloiu Fawzia Sha’at Corina Bubueanu Mihaela Deaconu Georgeta Neagu Mousa Sha’at Mihai Anastasescu Mona Mihailescu Cristian Matei Gheorghe Nechifor Daniela Berger |
| author_sort | Ramona-Daniela Păvăloiu |
| collection | DOAJ |
| description | The paper deals with the preparation and characterisation of hydroalcoholic polyphenolic extract from <i>Sambucus ebulus</i> (SE) leaves that was further loaded into three-types of lipid vesicles: liposomes, transfersomes, and ethosomes, to improve its bioavailability and achieve an optimum pharmacological effect. For <i>Sambucus ebulus</i> L.-loaded lipid vesicles, the entrapment efficiency, particle size, polydispersity index and stability were determined. All prepared lipid vesicles showed a good entrapment efficiency, in the range of 75−85%, nanometric size, low polydispersity indexes, and good stability over three months at 4 °C. The in vitro polyphenols released from lipid vehicles demonstrated slower kinetics when compared to the free extract dissolution in phosphate buffer solution at pH 7.4. Either free SE extract or SE extract loaded into lipid vesicles demonstrated a cytoprotective effect, even at low concentration, 5 ug/mL, against hydrogen peroxide-induced toxicity on L-929 mouse fibroblasts’ cell lines. However, the cytoprotective effect depended on the time of the cells pre-treatment with SE extract before exposure to a hydrogen peroxide solution of 50 mM concentration, requiring at least 12 h of pre-treatment with polyphenols with radical scavenging capacity. |
| first_indexed | 2024-04-13T02:49:16Z |
| format | Article |
| id | doaj.art-22413c6a4cbe4e1b966f0b0185b78f1f |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-04-13T02:49:16Z |
| publishDate | 2019-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-22413c6a4cbe4e1b966f0b0185b78f1f2022-12-22T03:05:54ZengMDPI AGNanomaterials2079-49912019-12-011015610.3390/nano10010056nano10010056Polyphenolic Extract from <i>Sambucus ebulus</i> L. Leaves Free and Loaded into Lipid VesiclesRamona-Daniela Păvăloiu0Fawzia Sha’at1Corina Bubueanu2Mihaela Deaconu3Georgeta Neagu4Mousa Sha’at5Mihai Anastasescu6Mona Mihailescu7Cristian Matei8Gheorghe Nechifor9Daniela Berger10National Institute for Chemical-Pharmaceutical Research and Development-ICCF Bucharest, Vitan Avenue 112, 031299 Bucharest, RomaniaNational Institute for Chemical-Pharmaceutical Research and Development-ICCF Bucharest, Vitan Avenue 112, 031299 Bucharest, RomaniaNational Institute for Chemical-Pharmaceutical Research and Development-ICCF Bucharest, Vitan Avenue 112, 031299 Bucharest, RomaniaFaculty of Applied Chemistry and Materials Science, University “Politehnica” of Bucharest, 1–7 Gheorghe Polizu St., 011061 Bucharest, RomaniaNational Institute for Chemical-Pharmaceutical Research and Development-ICCF Bucharest, Vitan Avenue 112, 031299 Bucharest, RomaniaFaculty of Pharmacy, University of Medicine and Pharmacy Grigore T. Popa, Iasi, Universitatii Avenue, 16, 700115 Iasi, Romania“Ilie Murgulescu” Institute of Physical-Chemistry, Romanian Academy, Splaiul Independentei no. 202, 060021 Bucharest, RomaniaPhysics Department, University “Politehnica” of Bucharest, Splaiul Independetei no 313, 060042 Bucharest, RomaniaFaculty of Applied Chemistry and Materials Science, University “Politehnica” of Bucharest, 1–7 Gheorghe Polizu St., 011061 Bucharest, RomaniaFaculty of Applied Chemistry and Materials Science, University “Politehnica” of Bucharest, 1–7 Gheorghe Polizu St., 011061 Bucharest, RomaniaFaculty of Applied Chemistry and Materials Science, University “Politehnica” of Bucharest, 1–7 Gheorghe Polizu St., 011061 Bucharest, RomaniaThe paper deals with the preparation and characterisation of hydroalcoholic polyphenolic extract from <i>Sambucus ebulus</i> (SE) leaves that was further loaded into three-types of lipid vesicles: liposomes, transfersomes, and ethosomes, to improve its bioavailability and achieve an optimum pharmacological effect. For <i>Sambucus ebulus</i> L.-loaded lipid vesicles, the entrapment efficiency, particle size, polydispersity index and stability were determined. All prepared lipid vesicles showed a good entrapment efficiency, in the range of 75−85%, nanometric size, low polydispersity indexes, and good stability over three months at 4 °C. The in vitro polyphenols released from lipid vehicles demonstrated slower kinetics when compared to the free extract dissolution in phosphate buffer solution at pH 7.4. Either free SE extract or SE extract loaded into lipid vesicles demonstrated a cytoprotective effect, even at low concentration, 5 ug/mL, against hydrogen peroxide-induced toxicity on L-929 mouse fibroblasts’ cell lines. However, the cytoprotective effect depended on the time of the cells pre-treatment with SE extract before exposure to a hydrogen peroxide solution of 50 mM concentration, requiring at least 12 h of pre-treatment with polyphenols with radical scavenging capacity.https://www.mdpi.com/2079-4991/10/1/56<i>sambucus ebulus</i>lipid vesiclespolyphenolscytoprotective effect |
| spellingShingle | Ramona-Daniela Păvăloiu Fawzia Sha’at Corina Bubueanu Mihaela Deaconu Georgeta Neagu Mousa Sha’at Mihai Anastasescu Mona Mihailescu Cristian Matei Gheorghe Nechifor Daniela Berger Polyphenolic Extract from <i>Sambucus ebulus</i> L. Leaves Free and Loaded into Lipid Vesicles Nanomaterials <i>sambucus ebulus</i> lipid vesicles polyphenols cytoprotective effect |
| title | Polyphenolic Extract from <i>Sambucus ebulus</i> L. Leaves Free and Loaded into Lipid Vesicles |
| title_full | Polyphenolic Extract from <i>Sambucus ebulus</i> L. Leaves Free and Loaded into Lipid Vesicles |
| title_fullStr | Polyphenolic Extract from <i>Sambucus ebulus</i> L. Leaves Free and Loaded into Lipid Vesicles |
| title_full_unstemmed | Polyphenolic Extract from <i>Sambucus ebulus</i> L. Leaves Free and Loaded into Lipid Vesicles |
| title_short | Polyphenolic Extract from <i>Sambucus ebulus</i> L. Leaves Free and Loaded into Lipid Vesicles |
| title_sort | polyphenolic extract from i sambucus ebulus i l leaves free and loaded into lipid vesicles |
| topic | <i>sambucus ebulus</i> lipid vesicles polyphenols cytoprotective effect |
| url | https://www.mdpi.com/2079-4991/10/1/56 |
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