Cloning and computer analysis of the promoter region of the legumin-like storage protein gene from buckwheat, Fagopyrum esculentum Moench
Using the modified 5’-RACE approach, a fragment containing the 955 bp long 5’- regulatory region of the buckwheat storage globulin gene (FeLEG1) has been amplified from the genomic DNA of buckwheat. The entire fragment was sequenced and the sequence analyzed by computer prediction of cis-regulatory...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2004-01-01
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| Series: | Archives of Biological Sciences |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2004/0354-46640402001M.pdf |
| _version_ | 1811300172740689920 |
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| author | Milisavljević Mira Konstantinović Miroslav M. Brkljačić Jelena M. Maksimović Vesna R. |
| author_facet | Milisavljević Mira Konstantinović Miroslav M. Brkljačić Jelena M. Maksimović Vesna R. |
| author_sort | Milisavljević Mira |
| collection | DOAJ |
| description | Using the modified 5’-RACE approach, a fragment containing the 955 bp long 5’- regulatory region of the buckwheat storage globulin gene (FeLEG1) has been amplified from the genomic DNA of buckwheat. The entire fragment was sequenced and the sequence analyzed by computer prediction of cis-regulatory elements possibly involved in tissue specific and developmentally controlled seed storage protein gene expression. The promoter obtained might be interesting not only for fundamental research, but also as a useful tool for biotechnological application. |
| first_indexed | 2024-04-13T06:47:19Z |
| format | Article |
| id | doaj.art-224605c3d256486296bd9119e71c71ba |
| institution | Directory Open Access Journal |
| issn | 0354-4664 |
| language | English |
| last_indexed | 2024-04-13T06:47:19Z |
| publishDate | 2004-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-224605c3d256486296bd9119e71c71ba2022-12-22T02:57:30ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46642004-01-01561-21710.2298/ABS0402001MCloning and computer analysis of the promoter region of the legumin-like storage protein gene from buckwheat, Fagopyrum esculentum MoenchMilisavljević MiraKonstantinović Miroslav M.Brkljačić Jelena M.Maksimović Vesna R.Using the modified 5’-RACE approach, a fragment containing the 955 bp long 5’- regulatory region of the buckwheat storage globulin gene (FeLEG1) has been amplified from the genomic DNA of buckwheat. The entire fragment was sequenced and the sequence analyzed by computer prediction of cis-regulatory elements possibly involved in tissue specific and developmentally controlled seed storage protein gene expression. The promoter obtained might be interesting not only for fundamental research, but also as a useful tool for biotechnological application.http://www.doiserbia.nb.rs/img/doi/0354-4664/2004/0354-46640402001M.pdf |
| spellingShingle | Milisavljević Mira Konstantinović Miroslav M. Brkljačić Jelena M. Maksimović Vesna R. Cloning and computer analysis of the promoter region of the legumin-like storage protein gene from buckwheat, Fagopyrum esculentum Moench Archives of Biological Sciences |
| title | Cloning and computer analysis of the promoter region of the legumin-like storage protein gene from buckwheat, Fagopyrum esculentum Moench |
| title_full | Cloning and computer analysis of the promoter region of the legumin-like storage protein gene from buckwheat, Fagopyrum esculentum Moench |
| title_fullStr | Cloning and computer analysis of the promoter region of the legumin-like storage protein gene from buckwheat, Fagopyrum esculentum Moench |
| title_full_unstemmed | Cloning and computer analysis of the promoter region of the legumin-like storage protein gene from buckwheat, Fagopyrum esculentum Moench |
| title_short | Cloning and computer analysis of the promoter region of the legumin-like storage protein gene from buckwheat, Fagopyrum esculentum Moench |
| title_sort | cloning and computer analysis of the promoter region of the legumin like storage protein gene from buckwheat fagopyrum esculentum moench |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2004/0354-46640402001M.pdf |
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