Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing <i>Plasmodium berghei</i> MSP-8, MSP-9 and RAP1
Virus-like particles (VLP) are a highly efficient vaccine platform used to present multiple antigenic proteins. Merozoite surface protein 8 (MSP-8), 9 (MSP-9) and rhoptry-associated protein 1 (RAP1) of <i>Plasmodium berghei</i> are the important proteins in erythrocyte invasion and the r...
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MDPI AG
2022-01-01
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| Series: | Vaccines |
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| author | Su-Hwa Lee Ki-Back Chu Hae-Ji Kang Fu-Shi Quan |
| author_facet | Su-Hwa Lee Ki-Back Chu Hae-Ji Kang Fu-Shi Quan |
| author_sort | Su-Hwa Lee |
| collection | DOAJ |
| description | Virus-like particles (VLP) are a highly efficient vaccine platform used to present multiple antigenic proteins. Merozoite surface protein 8 (MSP-8), 9 (MSP-9) and rhoptry-associated protein 1 (RAP1) of <i>Plasmodium berghei</i> are the important proteins in erythrocyte invasion and the replication of parasites. In this study, we generated three VLPs expressing MSP-8, MSP-9 or RAP1 together with influenza virus matrix protein M1 as a core protein, and the protection and alleviated inflammation induced by VLP immunization were investigated. Mice were immunized with a mixture of three VLPs, MSP-8, MSP-9 and RAP1, and challenge-infected with <i>P. berghei</i>. As a result, VLPs immunization elicited higher levels of <i>P. berghei</i> or VLPs-specific IgG antibody responses in the sera upon boost compared to that upon prime and naive. Upon challenge infection with <i>P. berghei</i>, higher levels of CD4+ T cell and memory B cell responses in the spleen were also found in VLPs-immunized mice compared to non-immunized control. Importantly, VLP immunization significantly alleviated inflammatory cytokine responses (TNF-α, IFN-γ) both in the sera and spleen. VLP vaccine immunization also assisted in diminishing the parasitic burden in the peripheral blood and prolonged the survival of immunized mice. These results indicated that a VLPs vaccine containing MSP-8, MSP-9 and RAP1 could be a vaccine candidate for <i>P. berghei</i> infection. |
| first_indexed | 2024-03-09T20:54:14Z |
| format | Article |
| id | doaj.art-2246b9ef5c9b4c939a76a1857cd74d6d |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T20:54:14Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-2246b9ef5c9b4c939a76a1857cd74d6d2023-11-23T22:25:12ZengMDPI AGVaccines2076-393X2022-01-0110220310.3390/vaccines10020203Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing <i>Plasmodium berghei</i> MSP-8, MSP-9 and RAP1Su-Hwa Lee0Ki-Back Chu1Hae-Ji Kang2Fu-Shi Quan3Department of Medical Zoology, School of Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Medical Zoology, School of Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Medical Zoology, School of Medicine, Kyung Hee University, Seoul 02447, KoreaVirus-like particles (VLP) are a highly efficient vaccine platform used to present multiple antigenic proteins. Merozoite surface protein 8 (MSP-8), 9 (MSP-9) and rhoptry-associated protein 1 (RAP1) of <i>Plasmodium berghei</i> are the important proteins in erythrocyte invasion and the replication of parasites. In this study, we generated three VLPs expressing MSP-8, MSP-9 or RAP1 together with influenza virus matrix protein M1 as a core protein, and the protection and alleviated inflammation induced by VLP immunization were investigated. Mice were immunized with a mixture of three VLPs, MSP-8, MSP-9 and RAP1, and challenge-infected with <i>P. berghei</i>. As a result, VLPs immunization elicited higher levels of <i>P. berghei</i> or VLPs-specific IgG antibody responses in the sera upon boost compared to that upon prime and naive. Upon challenge infection with <i>P. berghei</i>, higher levels of CD4+ T cell and memory B cell responses in the spleen were also found in VLPs-immunized mice compared to non-immunized control. Importantly, VLP immunization significantly alleviated inflammatory cytokine responses (TNF-α, IFN-γ) both in the sera and spleen. VLP vaccine immunization also assisted in diminishing the parasitic burden in the peripheral blood and prolonged the survival of immunized mice. These results indicated that a VLPs vaccine containing MSP-8, MSP-9 and RAP1 could be a vaccine candidate for <i>P. berghei</i> infection.https://www.mdpi.com/2076-393X/10/2/203<i>Plasmodium berghei</i>merozoite surface protein 8merozoite surface protein 9rhoptry-associated protein 1virus-like particlevaccine |
| spellingShingle | Su-Hwa Lee Ki-Back Chu Hae-Ji Kang Fu-Shi Quan Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing <i>Plasmodium berghei</i> MSP-8, MSP-9 and RAP1 Vaccines <i>Plasmodium berghei</i> merozoite surface protein 8 merozoite surface protein 9 rhoptry-associated protein 1 virus-like particle vaccine |
| title | Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing <i>Plasmodium berghei</i> MSP-8, MSP-9 and RAP1 |
| title_full | Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing <i>Plasmodium berghei</i> MSP-8, MSP-9 and RAP1 |
| title_fullStr | Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing <i>Plasmodium berghei</i> MSP-8, MSP-9 and RAP1 |
| title_full_unstemmed | Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing <i>Plasmodium berghei</i> MSP-8, MSP-9 and RAP1 |
| title_short | Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing <i>Plasmodium berghei</i> MSP-8, MSP-9 and RAP1 |
| title_sort | protection and alleviated inflammation induced by virus like particle vaccines containing i plasmodium berghei i msp 8 msp 9 and rap1 |
| topic | <i>Plasmodium berghei</i> merozoite surface protein 8 merozoite surface protein 9 rhoptry-associated protein 1 virus-like particle vaccine |
| url | https://www.mdpi.com/2076-393X/10/2/203 |
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