Functional role of PGAM5 multimeric assemblies and their polymerization into filaments
PGAM5 is a mitochondrial protein phosphatase whose functions include regulation of mitophagy and cell death. Here, the authors use x-ray crystallography and EM to show that PGAM5 forms dodecameric rings and filaments in solution, and find that PGAM5 rings are essential for catalysis and for a struct...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2019-01-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-08393-w |
| _version_ | 1819036183180607488 |
|---|---|
| author | Karen Ruiz Tarjani M. Thaker Christopher Agnew Lakshmi Miller-Vedam Raphael Trenker Clara Herrera Maria Ingaramo Daniel Toso Adam Frost Natalia Jura |
| author_facet | Karen Ruiz Tarjani M. Thaker Christopher Agnew Lakshmi Miller-Vedam Raphael Trenker Clara Herrera Maria Ingaramo Daniel Toso Adam Frost Natalia Jura |
| author_sort | Karen Ruiz |
| collection | DOAJ |
| description | PGAM5 is a mitochondrial protein phosphatase whose functions include regulation of mitophagy and cell death. Here, the authors use x-ray crystallography and EM to show that PGAM5 forms dodecameric rings and filaments in solution, and find that PGAM5 rings are essential for catalysis and for a structural effect PGAM5 has on mitochondrial membranes, independently of catalytic activity. |
| first_indexed | 2024-12-21T08:01:28Z |
| format | Article |
| id | doaj.art-224769ad2a28455cbf308ca51e75d047 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-21T08:01:28Z |
| publishDate | 2019-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-224769ad2a28455cbf308ca51e75d0472022-12-21T19:10:54ZengNature PortfolioNature Communications2041-17232019-01-0110111610.1038/s41467-019-08393-wFunctional role of PGAM5 multimeric assemblies and their polymerization into filamentsKaren Ruiz0Tarjani M. Thaker1Christopher Agnew2Lakshmi Miller-Vedam3Raphael Trenker4Clara Herrera5Maria Ingaramo6Daniel Toso7Adam Frost8Natalia Jura9Cardiovascular Research Institute, University of California San FranciscoCardiovascular Research Institute, University of California San FranciscoCardiovascular Research Institute, University of California San FranciscoDepartment of Biochemistry and Biophysics, University of California San FranciscoCardiovascular Research Institute, University of California San FranciscoTetrad Graduate Program, University of California San FranciscoCalico Life SciencesCalifornia Institute for Quantitative Biosciences, University of California BerkeleyDepartment of Biochemistry and Biophysics, University of California San FranciscoCardiovascular Research Institute, University of California San FranciscoPGAM5 is a mitochondrial protein phosphatase whose functions include regulation of mitophagy and cell death. Here, the authors use x-ray crystallography and EM to show that PGAM5 forms dodecameric rings and filaments in solution, and find that PGAM5 rings are essential for catalysis and for a structural effect PGAM5 has on mitochondrial membranes, independently of catalytic activity.https://doi.org/10.1038/s41467-019-08393-w |
| spellingShingle | Karen Ruiz Tarjani M. Thaker Christopher Agnew Lakshmi Miller-Vedam Raphael Trenker Clara Herrera Maria Ingaramo Daniel Toso Adam Frost Natalia Jura Functional role of PGAM5 multimeric assemblies and their polymerization into filaments Nature Communications |
| title | Functional role of PGAM5 multimeric assemblies and their polymerization into filaments |
| title_full | Functional role of PGAM5 multimeric assemblies and their polymerization into filaments |
| title_fullStr | Functional role of PGAM5 multimeric assemblies and their polymerization into filaments |
| title_full_unstemmed | Functional role of PGAM5 multimeric assemblies and their polymerization into filaments |
| title_short | Functional role of PGAM5 multimeric assemblies and their polymerization into filaments |
| title_sort | functional role of pgam5 multimeric assemblies and their polymerization into filaments |
| url | https://doi.org/10.1038/s41467-019-08393-w |
| work_keys_str_mv | AT karenruiz functionalroleofpgam5multimericassembliesandtheirpolymerizationintofilaments AT tarjanimthaker functionalroleofpgam5multimericassembliesandtheirpolymerizationintofilaments AT christopheragnew functionalroleofpgam5multimericassembliesandtheirpolymerizationintofilaments AT lakshmimillervedam functionalroleofpgam5multimericassembliesandtheirpolymerizationintofilaments AT raphaeltrenker functionalroleofpgam5multimericassembliesandtheirpolymerizationintofilaments AT claraherrera functionalroleofpgam5multimericassembliesandtheirpolymerizationintofilaments AT mariaingaramo functionalroleofpgam5multimericassembliesandtheirpolymerizationintofilaments AT danieltoso functionalroleofpgam5multimericassembliesandtheirpolymerizationintofilaments AT adamfrost functionalroleofpgam5multimericassembliesandtheirpolymerizationintofilaments AT nataliajura functionalroleofpgam5multimericassembliesandtheirpolymerizationintofilaments |