The Spleen Is an Ideal Site for Inducing Transplanted Islet Graft Expansion in Mice.
Alternative islet transplantation sites have the potential to reduce the marginal number of islets required to ameliorate hyperglycemia in recipients with diabetes. Previously, we reported that T cell leukemia homeobox 1 (Tlx1)+ stem cells in the spleen effectively regenerated into insulin-producing...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5279780?pdf=render |
| _version_ | 1818499582786535424 |
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| author | Takeshi Itoh Hitomi Nishinakamura Kenjiro Kumano Hiroyuki Takahashi Shohta Kodama |
| author_facet | Takeshi Itoh Hitomi Nishinakamura Kenjiro Kumano Hiroyuki Takahashi Shohta Kodama |
| author_sort | Takeshi Itoh |
| collection | DOAJ |
| description | Alternative islet transplantation sites have the potential to reduce the marginal number of islets required to ameliorate hyperglycemia in recipients with diabetes. Previously, we reported that T cell leukemia homeobox 1 (Tlx1)+ stem cells in the spleen effectively regenerated into insulin-producing cells in the pancreas of non-obese diabetic mice with end-stage disease. Thus, we investigated the spleen as a potential alternative islet transplantation site. Streptozotocin-induced diabetic C57BL/6 mice received syngeneic islets into the portal vein (PV), beneath the kidney capsule (KC), or into the spleen (SP). The marginal number of islets by PV, KC, or SP was 200, 100, and 50, respectively. Some plasma inflammatory cytokine levels in the SP group were significantly lower than those of the PV group after receiving a marginal number of islets, indicating reduced inflammation in the SP group. Insulin contents were increased 280 days after islet transplantation compared with those immediately following transplantation (p<0.05). Additionally, Tlx1-related genes, including Rrm2b and Pla2g2d, were up-regulated, which indicates that islet grafts expanded in the spleen. The spleen is an ideal candidate for an alternative islet transplantation site because of the resulting reduced inflammation and expansion of the islet graft. |
| first_indexed | 2024-12-10T20:31:28Z |
| format | Article |
| id | doaj.art-2251b1853b3142569a57fa25dff569c6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T20:31:28Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2251b1853b3142569a57fa25dff569c62022-12-22T01:34:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01121e017089910.1371/journal.pone.0170899The Spleen Is an Ideal Site for Inducing Transplanted Islet Graft Expansion in Mice.Takeshi ItohHitomi NishinakamuraKenjiro KumanoHiroyuki TakahashiShohta KodamaAlternative islet transplantation sites have the potential to reduce the marginal number of islets required to ameliorate hyperglycemia in recipients with diabetes. Previously, we reported that T cell leukemia homeobox 1 (Tlx1)+ stem cells in the spleen effectively regenerated into insulin-producing cells in the pancreas of non-obese diabetic mice with end-stage disease. Thus, we investigated the spleen as a potential alternative islet transplantation site. Streptozotocin-induced diabetic C57BL/6 mice received syngeneic islets into the portal vein (PV), beneath the kidney capsule (KC), or into the spleen (SP). The marginal number of islets by PV, KC, or SP was 200, 100, and 50, respectively. Some plasma inflammatory cytokine levels in the SP group were significantly lower than those of the PV group after receiving a marginal number of islets, indicating reduced inflammation in the SP group. Insulin contents were increased 280 days after islet transplantation compared with those immediately following transplantation (p<0.05). Additionally, Tlx1-related genes, including Rrm2b and Pla2g2d, were up-regulated, which indicates that islet grafts expanded in the spleen. The spleen is an ideal candidate for an alternative islet transplantation site because of the resulting reduced inflammation and expansion of the islet graft.http://europepmc.org/articles/PMC5279780?pdf=render |
| spellingShingle | Takeshi Itoh Hitomi Nishinakamura Kenjiro Kumano Hiroyuki Takahashi Shohta Kodama The Spleen Is an Ideal Site for Inducing Transplanted Islet Graft Expansion in Mice. PLoS ONE |
| title | The Spleen Is an Ideal Site for Inducing Transplanted Islet Graft Expansion in Mice. |
| title_full | The Spleen Is an Ideal Site for Inducing Transplanted Islet Graft Expansion in Mice. |
| title_fullStr | The Spleen Is an Ideal Site for Inducing Transplanted Islet Graft Expansion in Mice. |
| title_full_unstemmed | The Spleen Is an Ideal Site for Inducing Transplanted Islet Graft Expansion in Mice. |
| title_short | The Spleen Is an Ideal Site for Inducing Transplanted Islet Graft Expansion in Mice. |
| title_sort | spleen is an ideal site for inducing transplanted islet graft expansion in mice |
| url | http://europepmc.org/articles/PMC5279780?pdf=render |
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