Monkeypox virus genome sequence from an imported human case in Colombia
Introduction: Monkeypox virus (MPXV) is an enveloped double-stranded DNA virus with a genome of approximately 197.209 bp. The current classification divides MPXV into three clades: Clade I (Central African or Congo Basin clade) and clades IIa and IIb (West African clades). Objective: To report the c...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto Nacional de Salud
2022-09-01
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| Series: | Biomédica: revista del Instituto Nacional de Salud |
| Subjects: | |
| Online Access: | https://revistabiomedica.org/index.php/biomedica/article/view/6647 |
| Summary: | Introduction: Monkeypox virus (MPXV) is an enveloped double-stranded DNA virus with a genome of approximately 197.209 bp. The current classification divides MPXV into three clades: Clade I (Central African or Congo Basin clade) and clades IIa and IIb (West African clades).
Objective: To report the complete genome and phylogenetic analysis of a human monkeypox case detected in Colombia.
Materials and methods: Exudate from vesicular lesions was obtained from a male patient with recent travel history to Spain. A direct genomic approach was implemented in which total DNA from the sample was purified through a column-based method, followed by sequencing on the Nanopore GridION. Reads were aligned against the MPXV reference genome using minimap2 v.2.24 and phylogenetic inference was performed using maximum likelihood estimation.
Results: A total of 11.951 reads mapped directly to a reference genome with 96.8% of coverage (190.898 bp).
Conclusion: Phylogenetic analysis of the MPXV circulating in Colombia demonstrated its close relationship to clade IIb responsible for the multi-country outbreak in 2022. |
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| ISSN: | 0120-4157 2590-7379 |