Comparative Transcriptome Analysis of Salt Stress-Induced Leaf Senescence in Medicago truncatula
Leaves are the most critical portion of forage crops such as alfalfa (Medicago sativa). Leaf senescence caused by environmental stresses significantly impacts the biomass and quality of forages. To understand the molecular mechanisms and identify the key regulator of the salt stress-induced leaf sen...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.666660/full |
| _version_ | 1818727992377999360 |
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| author | Shuwei Dong Lijun Sang Hongli Xie Maofeng Chai Zeng-Yu Wang |
| author_facet | Shuwei Dong Lijun Sang Hongli Xie Maofeng Chai Zeng-Yu Wang |
| author_sort | Shuwei Dong |
| collection | DOAJ |
| description | Leaves are the most critical portion of forage crops such as alfalfa (Medicago sativa). Leaf senescence caused by environmental stresses significantly impacts the biomass and quality of forages. To understand the molecular mechanisms and identify the key regulator of the salt stress-induced leaf senescence process, we conducted a simple and effective salt stress-induced leaf senescence assay in Medicago truncatula, which was followed by RNA-Seq analysis coupled with physiological and biochemical characterization. By comparing the observed expression data with that derived from dark-induced leaf senescence at different time points, we identified 3,001, 3,787, and 4,419 senescence-associated genes (SAGs) for salt stress-induced leaf senescence on day 2, 4, and 6, respectively. There were 1546 SAGs shared by dark and salt stress treatment across the three time points. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses showed that the 1546 SAGs were mainly related to protein and amino acids metabolism, photosynthesis, chlorophyll metabolism, and hormone signaling during leaf senescence. Strikingly, many different transcription factors (TFs) families out of the 1546 SAGs, including NAC, bHLH, MYB, and ERF, were associated with salt stress-induced leaf senescence processes. Using the transient expression system in Nicotiana benthamiana, we verified that three functional NAC TF genes from the 1546 SAGs were related to leaf senescence. These results clarify SAGs under salt stress in M. truncatula and provide new insights and additional genetic resources for further forage crop breeding. |
| first_indexed | 2024-12-17T22:22:54Z |
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| id | doaj.art-225373d7cf58416e99588fe7aef0c3a0 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-17T22:22:54Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-225373d7cf58416e99588fe7aef0c3a02022-12-21T21:30:25ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-07-011210.3389/fpls.2021.666660666660Comparative Transcriptome Analysis of Salt Stress-Induced Leaf Senescence in Medicago truncatulaShuwei DongLijun SangHongli XieMaofeng ChaiZeng-Yu WangLeaves are the most critical portion of forage crops such as alfalfa (Medicago sativa). Leaf senescence caused by environmental stresses significantly impacts the biomass and quality of forages. To understand the molecular mechanisms and identify the key regulator of the salt stress-induced leaf senescence process, we conducted a simple and effective salt stress-induced leaf senescence assay in Medicago truncatula, which was followed by RNA-Seq analysis coupled with physiological and biochemical characterization. By comparing the observed expression data with that derived from dark-induced leaf senescence at different time points, we identified 3,001, 3,787, and 4,419 senescence-associated genes (SAGs) for salt stress-induced leaf senescence on day 2, 4, and 6, respectively. There were 1546 SAGs shared by dark and salt stress treatment across the three time points. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses showed that the 1546 SAGs were mainly related to protein and amino acids metabolism, photosynthesis, chlorophyll metabolism, and hormone signaling during leaf senescence. Strikingly, many different transcription factors (TFs) families out of the 1546 SAGs, including NAC, bHLH, MYB, and ERF, were associated with salt stress-induced leaf senescence processes. Using the transient expression system in Nicotiana benthamiana, we verified that three functional NAC TF genes from the 1546 SAGs were related to leaf senescence. These results clarify SAGs under salt stress in M. truncatula and provide new insights and additional genetic resources for further forage crop breeding.https://www.frontiersin.org/articles/10.3389/fpls.2021.666660/fullsalt stressleaf senescenceMedicago truncatulacomparative transcriptome analysisRNA-Seq |
| spellingShingle | Shuwei Dong Lijun Sang Hongli Xie Maofeng Chai Zeng-Yu Wang Comparative Transcriptome Analysis of Salt Stress-Induced Leaf Senescence in Medicago truncatula Frontiers in Plant Science salt stress leaf senescence Medicago truncatula comparative transcriptome analysis RNA-Seq |
| title | Comparative Transcriptome Analysis of Salt Stress-Induced Leaf Senescence in Medicago truncatula |
| title_full | Comparative Transcriptome Analysis of Salt Stress-Induced Leaf Senescence in Medicago truncatula |
| title_fullStr | Comparative Transcriptome Analysis of Salt Stress-Induced Leaf Senescence in Medicago truncatula |
| title_full_unstemmed | Comparative Transcriptome Analysis of Salt Stress-Induced Leaf Senescence in Medicago truncatula |
| title_short | Comparative Transcriptome Analysis of Salt Stress-Induced Leaf Senescence in Medicago truncatula |
| title_sort | comparative transcriptome analysis of salt stress induced leaf senescence in medicago truncatula |
| topic | salt stress leaf senescence Medicago truncatula comparative transcriptome analysis RNA-Seq |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.666660/full |
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