Allergenicity and structural properties of new Cor a 1 isoallergens from hazel identified in different plant tissues
Abstract The hazel allergen Cor a 1 is a PR-10 protein, closely related to the major birch pollen allergen Bet v 1. Hazel allergies are caused by cross-reactive IgE antibodies originally directed against Bet v 1. Despite the importance of PR-10 proteins in allergy development, their function and loc...
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-55856-2 |
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| author | Julian M. Hendrich Andreas Reuter Thessa P. Jacob Hatice Kara Sherine Amer Katharina Rödel Birgitta M. Wöhrl |
| author_facet | Julian M. Hendrich Andreas Reuter Thessa P. Jacob Hatice Kara Sherine Amer Katharina Rödel Birgitta M. Wöhrl |
| author_sort | Julian M. Hendrich |
| collection | DOAJ |
| description | Abstract The hazel allergen Cor a 1 is a PR-10 protein, closely related to the major birch pollen allergen Bet v 1. Hazel allergies are caused by cross-reactive IgE antibodies originally directed against Bet v 1. Despite the importance of PR-10 proteins in allergy development, their function and localization in the plant remain largely elusive. Therefore, the presence of Cor a 1 mRNA and proteins was investigated in different tissues, i.e., the female flower, immature and mature nuts, catkins, and pollen. Four yet unknown Cor a 1 isoallergens, i.e., Cor a 1.0501–1.0801, and one new Cor a 1.03 variant were discovered and characterized. Depending on the isoallergen, the occurrence and level of mRNA expression varied in different tissues, suggesting different functions. Interestingly, Cor a 1.04 previously thought to be only present in nuts, was also detected in catkins and pollen. The corresponding Cor a 1 genes were expressed in Escherichia coli. The purified proteins were analysed by CD and NMR spectroscopy. Immunoblots and ELISAs to determine their allergenic potential showed that the new proteins reacted positively with sera from patients allergic to birch, hazel and elder pollen and were recognized as novel isoallergens/variants by the WHO/IUIS Allergen Nomenclature Sub-Committee. |
| first_indexed | 2024-04-25T01:05:39Z |
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| id | doaj.art-2253bb5fe23d498fa142441de759c8c7 |
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| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-25T01:05:39Z |
| publishDate | 2024-03-01 |
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| spelling | doaj.art-2253bb5fe23d498fa142441de759c8c72024-03-10T12:12:16ZengNature PortfolioScientific Reports2045-23222024-03-0114111210.1038/s41598-024-55856-2Allergenicity and structural properties of new Cor a 1 isoallergens from hazel identified in different plant tissuesJulian M. Hendrich0Andreas Reuter1Thessa P. Jacob2Hatice Kara3Sherine Amer4Katharina Rödel5Birgitta M. Wöhrl6Lehrstuhl Biochemie IV - Biophysikalische Chemie, Universität BayreuthDivision of Allergology, Paul-Ehrlich-InstitutLehrstuhl Biochemie IV - Biophysikalische Chemie, Universität BayreuthLehrstuhl Biochemie IV - Biophysikalische Chemie, Universität BayreuthLehrstuhl Biochemie IV - Biophysikalische Chemie, Universität BayreuthLehrstuhl Biochemie IV - Biophysikalische Chemie, Universität BayreuthLehrstuhl Biochemie IV - Biophysikalische Chemie, Universität BayreuthAbstract The hazel allergen Cor a 1 is a PR-10 protein, closely related to the major birch pollen allergen Bet v 1. Hazel allergies are caused by cross-reactive IgE antibodies originally directed against Bet v 1. Despite the importance of PR-10 proteins in allergy development, their function and localization in the plant remain largely elusive. Therefore, the presence of Cor a 1 mRNA and proteins was investigated in different tissues, i.e., the female flower, immature and mature nuts, catkins, and pollen. Four yet unknown Cor a 1 isoallergens, i.e., Cor a 1.0501–1.0801, and one new Cor a 1.03 variant were discovered and characterized. Depending on the isoallergen, the occurrence and level of mRNA expression varied in different tissues, suggesting different functions. Interestingly, Cor a 1.04 previously thought to be only present in nuts, was also detected in catkins and pollen. The corresponding Cor a 1 genes were expressed in Escherichia coli. The purified proteins were analysed by CD and NMR spectroscopy. Immunoblots and ELISAs to determine their allergenic potential showed that the new proteins reacted positively with sera from patients allergic to birch, hazel and elder pollen and were recognized as novel isoallergens/variants by the WHO/IUIS Allergen Nomenclature Sub-Committee.https://doi.org/10.1038/s41598-024-55856-2PR-10 proteinBet v 1Cor a 1 isoallergensGene expressionLC-MSEHazel genome |
| spellingShingle | Julian M. Hendrich Andreas Reuter Thessa P. Jacob Hatice Kara Sherine Amer Katharina Rödel Birgitta M. Wöhrl Allergenicity and structural properties of new Cor a 1 isoallergens from hazel identified in different plant tissues Scientific Reports PR-10 protein Bet v 1 Cor a 1 isoallergens Gene expression LC-MSE Hazel genome |
| title | Allergenicity and structural properties of new Cor a 1 isoallergens from hazel identified in different plant tissues |
| title_full | Allergenicity and structural properties of new Cor a 1 isoallergens from hazel identified in different plant tissues |
| title_fullStr | Allergenicity and structural properties of new Cor a 1 isoallergens from hazel identified in different plant tissues |
| title_full_unstemmed | Allergenicity and structural properties of new Cor a 1 isoallergens from hazel identified in different plant tissues |
| title_short | Allergenicity and structural properties of new Cor a 1 isoallergens from hazel identified in different plant tissues |
| title_sort | allergenicity and structural properties of new cor a 1 isoallergens from hazel identified in different plant tissues |
| topic | PR-10 protein Bet v 1 Cor a 1 isoallergens Gene expression LC-MSE Hazel genome |
| url | https://doi.org/10.1038/s41598-024-55856-2 |
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