Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities
The demand for stem cell-based cultured meat as an alternative protein source is increasing in response to global food scarcity. However, the definition of quality controls, including appropriate growth factors and cell characteristics, remains incomplete. Cluster of differentiation (CD) 29 is ubiqu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/13/2/135 |
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| author | Yuna Naraoka Yo Mabuchi Mai Kiuchi Kyoko Kumagai Daisuke Hisamatsu Yosuke Yoneyama Takanori Takebe Chihiro Akazawa |
| author_facet | Yuna Naraoka Yo Mabuchi Mai Kiuchi Kyoko Kumagai Daisuke Hisamatsu Yosuke Yoneyama Takanori Takebe Chihiro Akazawa |
| author_sort | Yuna Naraoka |
| collection | DOAJ |
| description | The demand for stem cell-based cultured meat as an alternative protein source is increasing in response to global food scarcity. However, the definition of quality controls, including appropriate growth factors and cell characteristics, remains incomplete. Cluster of differentiation (CD) 29 is ubiquitously expressed in bovine muscle tissue and is a marker of progenitor cells in cultured meat. However, CD29+ cells are naturally heterogeneous, and this quality control issue must be resolved. In this study, the aim was to identify the subpopulation of the CD29+ cell population with potential utility in cultured meat production. The CD29+ cell population exhibited heterogeneity, discernible through the CD44 and CD344 markers. CD29+CD44−CD344− cells displayed the ability for long-term culture, demonstrating high adipogenic potential and substantial lipid droplet accumulation, even within 3D cultures. Conversely, CD29+CD44+ cells exhibited rapid proliferation but were not viable for prolonged culture. Using cells suitable for adipocyte and muscle differentiation, we successfully designed meat buds, especially those rich in fat. Collectively, the identification and comprehension of distinct cell populations within bovine tissues contribute to quality control predictions in meat production. They also aid in establishing a stable and reliable cultured meat production technique. |
| first_indexed | 2024-03-08T09:55:35Z |
| format | Article |
| id | doaj.art-2258112e8969487d92b3b515277d6eaa |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-08T09:55:35Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-2258112e8969487d92b3b515277d6eaa2024-01-29T13:50:16ZengMDPI AGCells2073-44092024-01-0113213510.3390/cells13020135Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation AbilitiesYuna Naraoka0Yo Mabuchi1Mai Kiuchi2Kyoko Kumagai3Daisuke Hisamatsu4Yosuke Yoneyama5Takanori Takebe6Chihiro Akazawa7Intractable Disease Research Center, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, JapanIntractable Disease Research Center, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, JapanIntractable Disease Research Center, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, JapanIntractable Disease Research Center, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, JapanIntractable Disease Research Center, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, JapanInstitute of Research, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, JapanInstitute of Research, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, JapanIntractable Disease Research Center, Juntendo University Graduate School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, JapanThe demand for stem cell-based cultured meat as an alternative protein source is increasing in response to global food scarcity. However, the definition of quality controls, including appropriate growth factors and cell characteristics, remains incomplete. Cluster of differentiation (CD) 29 is ubiquitously expressed in bovine muscle tissue and is a marker of progenitor cells in cultured meat. However, CD29+ cells are naturally heterogeneous, and this quality control issue must be resolved. In this study, the aim was to identify the subpopulation of the CD29+ cell population with potential utility in cultured meat production. The CD29+ cell population exhibited heterogeneity, discernible through the CD44 and CD344 markers. CD29+CD44−CD344− cells displayed the ability for long-term culture, demonstrating high adipogenic potential and substantial lipid droplet accumulation, even within 3D cultures. Conversely, CD29+CD44+ cells exhibited rapid proliferation but were not viable for prolonged culture. Using cells suitable for adipocyte and muscle differentiation, we successfully designed meat buds, especially those rich in fat. Collectively, the identification and comprehension of distinct cell populations within bovine tissues contribute to quality control predictions in meat production. They also aid in establishing a stable and reliable cultured meat production technique.https://www.mdpi.com/2073-4409/13/2/135stem cellsbovine cellsculture meatflow cytometryadipogenic differentiation |
| spellingShingle | Yuna Naraoka Yo Mabuchi Mai Kiuchi Kyoko Kumagai Daisuke Hisamatsu Yosuke Yoneyama Takanori Takebe Chihiro Akazawa Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities Cells stem cells bovine cells culture meat flow cytometry adipogenic differentiation |
| title | Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities |
| title_full | Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities |
| title_fullStr | Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities |
| title_full_unstemmed | Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities |
| title_short | Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities |
| title_sort | quality control of stem cell based cultured meat according to specific differentiation abilities |
| topic | stem cells bovine cells culture meat flow cytometry adipogenic differentiation |
| url | https://www.mdpi.com/2073-4409/13/2/135 |
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