Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies
Coccidiosis is an intestinal disease of chickens of major economic importance to broiler industries worldwide. Species of coccidia found in chickens include Eimeria acervulina, Eimeria brunetti, Eimeria maxima, Eimeria mitis, Eimeria necatrix, Eimeria praecox, and Eimeria tenella. In recent years, p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-01-01
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| Series: | Veterinary Sciences |
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| Online Access: | http://www.mdpi.com/2306-7381/5/1/12 |
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| author | Arianne Brown Jordan Damer Blake Jamila Beard Asha Beharry Louanne Serrette Atlyn Soleyn Jamie Sookhoo Lemar Blake Gabriel Brown Christopher Oura |
| author_facet | Arianne Brown Jordan Damer Blake Jamila Beard Asha Beharry Louanne Serrette Atlyn Soleyn Jamie Sookhoo Lemar Blake Gabriel Brown Christopher Oura |
| author_sort | Arianne Brown Jordan |
| collection | DOAJ |
| description | Coccidiosis is an intestinal disease of chickens of major economic importance to broiler industries worldwide. Species of coccidia found in chickens include Eimeria acervulina, Eimeria brunetti, Eimeria maxima, Eimeria mitis, Eimeria necatrix, Eimeria praecox, and Eimeria tenella. In recent years, polymerase chain reaction (PCR) has been developed to provide accurate and rapid identification of the seven known Eimeria species of chickens. The aim of this study was to use species-specific real-time PCR (qPCR) to identify which of the seven Eimeria species are present in Trinidad poultry. Seventeen pooled fecal samples were collected from 6 broiler farms (2–5 pens per farm) across Trinidad. Feces were also collected from birds showing clinical signs of coccidiosis in two live bird markets (pluck shops). qPCR revealed the presence of five species of Eimeria (E. acervulina, E. maxima, E. mitis, E. necatrix, and E. tenella), but not E. brunetti or E. praecox. Mixed infections were detected on all broiler farms, and DNA of two highly pathogenic Eimeria species (E. tenella and E. necatrix) was detected in feces taken from clinically sick birds sampled from the two pluck shops. |
| first_indexed | 2024-12-17T21:53:21Z |
| format | Article |
| id | doaj.art-2259bcf949c541fc81bf306c3b111ba3 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-12-17T21:53:21Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-2259bcf949c541fc81bf306c3b111ba32022-12-21T21:31:12ZengMDPI AGVeterinary Sciences2306-73812018-01-01511210.3390/vetsci5010012vetsci5010012Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West IndiesArianne Brown Jordan0Damer Blake1Jamila Beard2Asha Beharry3Louanne Serrette4Atlyn Soleyn5Jamie Sookhoo6Lemar Blake7Gabriel Brown8Christopher Oura9Department of Basic Veterinary Sciences, School of Veterinary Medicine, The University of the West Indies (St. Augustine), Eric Williams Medical Sciences Complex, Mount Hope, Republic of Trinidad and TobagoDepartment of Pathobiology and Population Sciences, The Royal Veterinary College, Hertfordshire AL9 7TA, UKSchool of Veterinary Medicine, The University of the West Indies (St. Augustine), Eric Williams Medical Sciences Complex, Mount Hope, Republic of Trinidad and TobagoSchool of Veterinary Medicine, The University of the West Indies (St. Augustine), Eric Williams Medical Sciences Complex, Mount Hope, Republic of Trinidad and TobagoSchool of Veterinary Medicine, The University of the West Indies (St. Augustine), Eric Williams Medical Sciences Complex, Mount Hope, Republic of Trinidad and TobagoSchool of Veterinary Medicine, The University of the West Indies (St. Augustine), Eric Williams Medical Sciences Complex, Mount Hope, Republic of Trinidad and TobagoDepartment of Basic Veterinary Sciences, School of Veterinary Medicine, The University of the West Indies (St. Augustine), Eric Williams Medical Sciences Complex, Mount Hope, Republic of Trinidad and TobagoDepartment of Basic Veterinary Sciences, School of Veterinary Medicine, The University of the West Indies (St. Augustine), Eric Williams Medical Sciences Complex, Mount Hope, Republic of Trinidad and TobagoDepartment of Veterinary Clinical Sciences, School of Veterinary Medicine, The University of the West Indies (St. Augustine), Eric Williams Medical Sciences Complex, Mount Hope, Republic of Trinidad and TobagoDepartment of Basic Veterinary Sciences, School of Veterinary Medicine, The University of the West Indies (St. Augustine), Eric Williams Medical Sciences Complex, Mount Hope, Republic of Trinidad and TobagoCoccidiosis is an intestinal disease of chickens of major economic importance to broiler industries worldwide. Species of coccidia found in chickens include Eimeria acervulina, Eimeria brunetti, Eimeria maxima, Eimeria mitis, Eimeria necatrix, Eimeria praecox, and Eimeria tenella. In recent years, polymerase chain reaction (PCR) has been developed to provide accurate and rapid identification of the seven known Eimeria species of chickens. The aim of this study was to use species-specific real-time PCR (qPCR) to identify which of the seven Eimeria species are present in Trinidad poultry. Seventeen pooled fecal samples were collected from 6 broiler farms (2–5 pens per farm) across Trinidad. Feces were also collected from birds showing clinical signs of coccidiosis in two live bird markets (pluck shops). qPCR revealed the presence of five species of Eimeria (E. acervulina, E. maxima, E. mitis, E. necatrix, and E. tenella), but not E. brunetti or E. praecox. Mixed infections were detected on all broiler farms, and DNA of two highly pathogenic Eimeria species (E. tenella and E. necatrix) was detected in feces taken from clinically sick birds sampled from the two pluck shops.http://www.mdpi.com/2306-7381/5/1/12coccidiosiscoccidiaEimeriapoultryTrinidad |
| spellingShingle | Arianne Brown Jordan Damer Blake Jamila Beard Asha Beharry Louanne Serrette Atlyn Soleyn Jamie Sookhoo Lemar Blake Gabriel Brown Christopher Oura Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies Veterinary Sciences coccidiosis coccidia Eimeria poultry Trinidad |
| title | Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies |
| title_full | Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies |
| title_fullStr | Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies |
| title_full_unstemmed | Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies |
| title_short | Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies |
| title_sort | molecular identification of eimeria species in broiler chickens in trinidad west indies |
| topic | coccidiosis coccidia Eimeria poultry Trinidad |
| url | http://www.mdpi.com/2306-7381/5/1/12 |
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