Alantolactone pyrazoline analogue inhibits cancer cell proliferation and induces apoptosis in non-small cell lung carcinoma cells
The aim of the current study was to evaluate the anticancer and apoptotic effects of alantolactone pyrazoline analogue in human non-small cell lung cancer (NCI-H460) cells. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zolium bromide) assay was used to evaluate the cell viability while as fluo...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bangladesh Pharmacological Society
2015-05-01
|
| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/22619 |
| _version_ | 1830212641624162304 |
|---|---|
| author | Jing lv Ming-Qin Cao Jian-Chun Yu |
| author_facet | Jing lv Ming-Qin Cao Jian-Chun Yu |
| author_sort | Jing lv |
| collection | DOAJ |
| description | The aim of the current study was to evaluate the anticancer and apoptotic effects of alantolactone pyrazoline analogue in human non-small cell lung cancer (NCI-H460) cells. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zolium bromide) assay was used to evaluate the cell viability while as fluorescence microscopy was used to assess the effect on apoptosis, cellular and nuclear morphology. Flow cytometry evaluated the effect of APA on cell cycle arrest in these cells. The results revealed that APA induced potent, time and dose-dependent cytotoxic effects on the growth of NCI-H460 cells. It also inhibited colony forming tendency as well as cell invasion capability of these cancer cells. APA induced dose-dependent nuclear and cellular morphological effects including chromatin condensation and DNA fragmentation. Flow cytometry revealed that the anticancer effects of APA might be due to its cell cycle arrest inducing tendency in G0/G1 phase of the cell cycle. |
| first_indexed | 2024-12-18T06:06:41Z |
| format | Article |
| id | doaj.art-2259c65a7b64456f95eaac96c6618ce0 |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-12-18T06:06:41Z |
| publishDate | 2015-05-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-2259c65a7b64456f95eaac96c6618ce02022-12-21T21:18:30ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882015-05-0110210.3329/bjp.v10i2.22619Alantolactone pyrazoline analogue inhibits cancer cell proliferation and induces apoptosis in non-small cell lung carcinoma cellsJing lv0Ming-Qin Cao1Jian-Chun Yu2Department of Oncology, The Affiliated Hospital of Qingdao University, Qingdao 266000Health Examination Center, Dongying People?s Hospital, Dongying 257091Department of Emergency, Linzi District People?s Hospital, Zibo 255400The aim of the current study was to evaluate the anticancer and apoptotic effects of alantolactone pyrazoline analogue in human non-small cell lung cancer (NCI-H460) cells. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zolium bromide) assay was used to evaluate the cell viability while as fluorescence microscopy was used to assess the effect on apoptosis, cellular and nuclear morphology. Flow cytometry evaluated the effect of APA on cell cycle arrest in these cells. The results revealed that APA induced potent, time and dose-dependent cytotoxic effects on the growth of NCI-H460 cells. It also inhibited colony forming tendency as well as cell invasion capability of these cancer cells. APA induced dose-dependent nuclear and cellular morphological effects including chromatin condensation and DNA fragmentation. Flow cytometry revealed that the anticancer effects of APA might be due to its cell cycle arrest inducing tendency in G0/G1 phase of the cell cycle.https://www.banglajol.info/index.php/BJP/article/view/22619Alantolactone pyrazoline analogueAnti-cancerApoptosisCell cycleLung cancer |
| spellingShingle | Jing lv Ming-Qin Cao Jian-Chun Yu Alantolactone pyrazoline analogue inhibits cancer cell proliferation and induces apoptosis in non-small cell lung carcinoma cells Bangladesh Journal of Pharmacology Alantolactone pyrazoline analogue Anti-cancer Apoptosis Cell cycle Lung cancer |
| title | Alantolactone pyrazoline analogue inhibits cancer cell proliferation and induces apoptosis in non-small cell lung carcinoma cells |
| title_full | Alantolactone pyrazoline analogue inhibits cancer cell proliferation and induces apoptosis in non-small cell lung carcinoma cells |
| title_fullStr | Alantolactone pyrazoline analogue inhibits cancer cell proliferation and induces apoptosis in non-small cell lung carcinoma cells |
| title_full_unstemmed | Alantolactone pyrazoline analogue inhibits cancer cell proliferation and induces apoptosis in non-small cell lung carcinoma cells |
| title_short | Alantolactone pyrazoline analogue inhibits cancer cell proliferation and induces apoptosis in non-small cell lung carcinoma cells |
| title_sort | alantolactone pyrazoline analogue inhibits cancer cell proliferation and induces apoptosis in non small cell lung carcinoma cells |
| topic | Alantolactone pyrazoline analogue Anti-cancer Apoptosis Cell cycle Lung cancer |
| url | https://www.banglajol.info/index.php/BJP/article/view/22619 |
| work_keys_str_mv | AT jinglv alantolactonepyrazolineanalogueinhibitscancercellproliferationandinducesapoptosisinnonsmallcelllungcarcinomacells AT mingqincao alantolactonepyrazolineanalogueinhibitscancercellproliferationandinducesapoptosisinnonsmallcelllungcarcinomacells AT jianchunyu alantolactonepyrazolineanalogueinhibitscancercellproliferationandinducesapoptosisinnonsmallcelllungcarcinomacells |