Comparative chromosome painting in six species of Oligoryzomys (Rodentia, Sigmodontinae) and the karyotype evolution of the genus.
Oligoryzomys belongs to the tribe Oryzomyini, and contains about 22 species. Diploid numbers range from 2n = 44 in Oligoryzomys sp. 2 to 2n = 72 in O. utiaritensis and phylogenetic relationships are not well defined. The high morphological convergence leads to misidentification of taxonomic entities...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4320059?pdf=render |
| _version_ | 1828412355116007424 |
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| author | Camilla Bruno Di-Nizo Karen Ventura Malcolm Andrew Ferguson-Smith Patricia Caroline Mary O'Brien Yatiyo Yonenaga-Yassuda Maria José de J Silva |
| author_facet | Camilla Bruno Di-Nizo Karen Ventura Malcolm Andrew Ferguson-Smith Patricia Caroline Mary O'Brien Yatiyo Yonenaga-Yassuda Maria José de J Silva |
| author_sort | Camilla Bruno Di-Nizo |
| collection | DOAJ |
| description | Oligoryzomys belongs to the tribe Oryzomyini, and contains about 22 species. Diploid numbers range from 2n = 44 in Oligoryzomys sp. 2 to 2n = 72 in O. utiaritensis and phylogenetic relationships are not well defined. The high morphological convergence leads to misidentification of taxonomic entities and the species are often identified by chromosomal characters. Until now, the genus has been studied only by classical cytogenetic approaches. To understand the chromosomal evolution of Oligoryzomys, we developed chromosome probes from a female of Oligoryzomys moojeni (OMO) with 2n = 70 and hybridized to other five Oligoryzomys species. The probes painted 31 segments on O. fornesi (OFO) with 2n = 62; 32 segments on O. microtis (OMI), 2n = 64; 33 segments on O. nigripes (ONI), 2n = 62 and on O. rupestris (ORU), 2n = 46; and 34 on Oligoryzomys sp. 2 (OSP), 2n = 44. OMO probes 4 and 5 showed a syntenic association in O. fornesi, O. microtis and O. nigripes and were also presented in the same pair, although disrupted, in O. rupestris and Oligoryzomys sp. 2. Concerning O. rupestris and Oligoryzomys sp. 2, species with the lowest diploid numbers of the genus, a total of 8 probes hybridized to 11 segments on the largest pair of ORU 1 and 9 probes hybridized to 12 segments on OSP 1. Also, OMO 6 painted three segments in ORU, corresponding to the proximal segment of ORU 2q, and the whole of ORU 19 and 20. In OSP, the segment corresponding to ORU 20 was homologous to OSP 1p. OMO X showed signals of hybridization in both X and Y chromosomes. Extensive chromosomal rearrangements, that could not be detected by classical cytogenetic techniques, such as pericentric inversions or repositioning of centromeres, Robertsonian rearrangements and tandem fusions/fissions, as well as gain/activation or loss/inactivation of centromeres and telomeric sequences have driven the huge genome reshuffling in these closely related species. |
| first_indexed | 2024-12-10T12:47:41Z |
| format | Article |
| id | doaj.art-225a7f9238194b3ca3aa472ab5257791 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T12:47:41Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-225a7f9238194b3ca3aa472ab52577912022-12-22T01:48:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011757910.1371/journal.pone.0117579Comparative chromosome painting in six species of Oligoryzomys (Rodentia, Sigmodontinae) and the karyotype evolution of the genus.Camilla Bruno Di-NizoKaren VenturaMalcolm Andrew Ferguson-SmithPatricia Caroline Mary O'BrienYatiyo Yonenaga-YassudaMaria José de J SilvaOligoryzomys belongs to the tribe Oryzomyini, and contains about 22 species. Diploid numbers range from 2n = 44 in Oligoryzomys sp. 2 to 2n = 72 in O. utiaritensis and phylogenetic relationships are not well defined. The high morphological convergence leads to misidentification of taxonomic entities and the species are often identified by chromosomal characters. Until now, the genus has been studied only by classical cytogenetic approaches. To understand the chromosomal evolution of Oligoryzomys, we developed chromosome probes from a female of Oligoryzomys moojeni (OMO) with 2n = 70 and hybridized to other five Oligoryzomys species. The probes painted 31 segments on O. fornesi (OFO) with 2n = 62; 32 segments on O. microtis (OMI), 2n = 64; 33 segments on O. nigripes (ONI), 2n = 62 and on O. rupestris (ORU), 2n = 46; and 34 on Oligoryzomys sp. 2 (OSP), 2n = 44. OMO probes 4 and 5 showed a syntenic association in O. fornesi, O. microtis and O. nigripes and were also presented in the same pair, although disrupted, in O. rupestris and Oligoryzomys sp. 2. Concerning O. rupestris and Oligoryzomys sp. 2, species with the lowest diploid numbers of the genus, a total of 8 probes hybridized to 11 segments on the largest pair of ORU 1 and 9 probes hybridized to 12 segments on OSP 1. Also, OMO 6 painted three segments in ORU, corresponding to the proximal segment of ORU 2q, and the whole of ORU 19 and 20. In OSP, the segment corresponding to ORU 20 was homologous to OSP 1p. OMO X showed signals of hybridization in both X and Y chromosomes. Extensive chromosomal rearrangements, that could not be detected by classical cytogenetic techniques, such as pericentric inversions or repositioning of centromeres, Robertsonian rearrangements and tandem fusions/fissions, as well as gain/activation or loss/inactivation of centromeres and telomeric sequences have driven the huge genome reshuffling in these closely related species.http://europepmc.org/articles/PMC4320059?pdf=render |
| spellingShingle | Camilla Bruno Di-Nizo Karen Ventura Malcolm Andrew Ferguson-Smith Patricia Caroline Mary O'Brien Yatiyo Yonenaga-Yassuda Maria José de J Silva Comparative chromosome painting in six species of Oligoryzomys (Rodentia, Sigmodontinae) and the karyotype evolution of the genus. PLoS ONE |
| title | Comparative chromosome painting in six species of Oligoryzomys (Rodentia, Sigmodontinae) and the karyotype evolution of the genus. |
| title_full | Comparative chromosome painting in six species of Oligoryzomys (Rodentia, Sigmodontinae) and the karyotype evolution of the genus. |
| title_fullStr | Comparative chromosome painting in six species of Oligoryzomys (Rodentia, Sigmodontinae) and the karyotype evolution of the genus. |
| title_full_unstemmed | Comparative chromosome painting in six species of Oligoryzomys (Rodentia, Sigmodontinae) and the karyotype evolution of the genus. |
| title_short | Comparative chromosome painting in six species of Oligoryzomys (Rodentia, Sigmodontinae) and the karyotype evolution of the genus. |
| title_sort | comparative chromosome painting in six species of oligoryzomys rodentia sigmodontinae and the karyotype evolution of the genus |
| url | http://europepmc.org/articles/PMC4320059?pdf=render |
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