Over-expression of Adenine Nucleotide Translocase 1 (ANT1) Induces Apoptosis and Tumor Regression <it>in vivo</it>
<p>Abstract</p> <p>Background</p> <p>Adenine nucleotide translocase (ANT) is located in the inner mitochondrial membrane and catalyzes the exchange of mitochondrial ATP for cytosolic ADP. ANT has been known to be a major component of the permeability transition pore com...
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| Format: | Article |
| Language: | English |
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BMC
2008-06-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/8/160 |
| _version_ | 1811287038574460928 |
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| author | Kim Chul-Woo Aung Khin Jeon Yoon-Kyung Choi Yun Jang Ji-Young |
| author_facet | Kim Chul-Woo Aung Khin Jeon Yoon-Kyung Choi Yun Jang Ji-Young |
| author_sort | Kim Chul-Woo |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Adenine nucleotide translocase (ANT) is located in the inner mitochondrial membrane and catalyzes the exchange of mitochondrial ATP for cytosolic ADP. ANT has been known to be a major component of the permeability transition pore complex of mitochondria and contributes to mitochondria-mediated apoptosis. Human ANT has four isoforms (ANT1, ANT2, ANT3, and ANT4), and the expression of the ANT isoforms is variable depending on the tissue and cell type, developmental stage, and proliferation status. Among the isoforms, ANT1 is highly expressed in terminally-differentiated tissues, but expressed in low levels in proliferating cells, such as cancer cells. In particular, over-expression of ANT1 induces apoptosis in cultured tumor cells.</p> <p>Methods</p> <p>We applied an ANT1 gene transfer approach to induce apoptosis and to evaluate the anti-tumor effect of ANT1 in a nude mouse model.</p> <p>Results</p> <p>We demonstrated that ANT1 transfection induced apoptosis of MDA-MB-231 cells, inactivated NF-κB activity, and increased Bax expression. ANT1-inducing apoptosis was accompanied by the disruption of mitochondrial membrane potential, cytochrome c release and the activation of caspases-9 and -3. Moreover, ANT1 transfection significantly suppressed tumor growth <it>in vivo</it>.</p> <p>Conclusion</p> <p>Our results suggest that ANT1 transfection may be a useful therapeutic modality for the treatment of cancer.</p> |
| first_indexed | 2024-04-13T03:11:43Z |
| format | Article |
| id | doaj.art-225bed89652342b5ba6b21d7a2b35723 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-13T03:11:43Z |
| publishDate | 2008-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-225bed89652342b5ba6b21d7a2b357232022-12-22T03:05:01ZengBMCBMC Cancer1471-24072008-06-018116010.1186/1471-2407-8-160Over-expression of Adenine Nucleotide Translocase 1 (ANT1) Induces Apoptosis and Tumor Regression <it>in vivo</it>Kim Chul-WooAung KhinJeon Yoon-KyungChoi YunJang Ji-Young<p>Abstract</p> <p>Background</p> <p>Adenine nucleotide translocase (ANT) is located in the inner mitochondrial membrane and catalyzes the exchange of mitochondrial ATP for cytosolic ADP. ANT has been known to be a major component of the permeability transition pore complex of mitochondria and contributes to mitochondria-mediated apoptosis. Human ANT has four isoforms (ANT1, ANT2, ANT3, and ANT4), and the expression of the ANT isoforms is variable depending on the tissue and cell type, developmental stage, and proliferation status. Among the isoforms, ANT1 is highly expressed in terminally-differentiated tissues, but expressed in low levels in proliferating cells, such as cancer cells. In particular, over-expression of ANT1 induces apoptosis in cultured tumor cells.</p> <p>Methods</p> <p>We applied an ANT1 gene transfer approach to induce apoptosis and to evaluate the anti-tumor effect of ANT1 in a nude mouse model.</p> <p>Results</p> <p>We demonstrated that ANT1 transfection induced apoptosis of MDA-MB-231 cells, inactivated NF-κB activity, and increased Bax expression. ANT1-inducing apoptosis was accompanied by the disruption of mitochondrial membrane potential, cytochrome c release and the activation of caspases-9 and -3. Moreover, ANT1 transfection significantly suppressed tumor growth <it>in vivo</it>.</p> <p>Conclusion</p> <p>Our results suggest that ANT1 transfection may be a useful therapeutic modality for the treatment of cancer.</p>http://www.biomedcentral.com/1471-2407/8/160 |
| spellingShingle | Kim Chul-Woo Aung Khin Jeon Yoon-Kyung Choi Yun Jang Ji-Young Over-expression of Adenine Nucleotide Translocase 1 (ANT1) Induces Apoptosis and Tumor Regression <it>in vivo</it> BMC Cancer |
| title | Over-expression of Adenine Nucleotide Translocase 1 (ANT1) Induces Apoptosis and Tumor Regression <it>in vivo</it> |
| title_full | Over-expression of Adenine Nucleotide Translocase 1 (ANT1) Induces Apoptosis and Tumor Regression <it>in vivo</it> |
| title_fullStr | Over-expression of Adenine Nucleotide Translocase 1 (ANT1) Induces Apoptosis and Tumor Regression <it>in vivo</it> |
| title_full_unstemmed | Over-expression of Adenine Nucleotide Translocase 1 (ANT1) Induces Apoptosis and Tumor Regression <it>in vivo</it> |
| title_short | Over-expression of Adenine Nucleotide Translocase 1 (ANT1) Induces Apoptosis and Tumor Regression <it>in vivo</it> |
| title_sort | over expression of adenine nucleotide translocase 1 ant1 induces apoptosis and tumor regression it in vivo it |
| url | http://www.biomedcentral.com/1471-2407/8/160 |
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