Taxonomic Structure Evolution, Chemical Composition and Anaerobic Digestibility of Microalgae-Bacterial Granular Sludge (M-BGS) Grown during Treatment of Digestate
The liquid fraction from the dewatering of digested sewage sludge (LF-DSS) represents a major processing complication for wastewater treatment facilities, thus necessitating new and effective methods of LF-DSS neutralization. This pilot-scale study examined the evolution of a <i>Chlorella</...
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MDPI AG
2023-01-01
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| author | Joanna Kazimierowicz Marcin Dębowski Marcin Zieliński |
| author_facet | Joanna Kazimierowicz Marcin Dębowski Marcin Zieliński |
| author_sort | Joanna Kazimierowicz |
| collection | DOAJ |
| description | The liquid fraction from the dewatering of digested sewage sludge (LF-DSS) represents a major processing complication for wastewater treatment facilities, thus necessitating new and effective methods of LF-DSS neutralization. This pilot-scale study examined the evolution of a <i>Chlorella</i> sp. monoculture into microalgal-bacterial granular sludge (M-BGS) during treatment of LF-DSS in a hybrid photo-bioreactor (H-PBR). The M-BGS reached a stable taxonomic and morphological structure after 60 days of H-PBR operation. The biomass was primarily composed of <i>Chlorella</i> sp., <i>Microthrix parvicella</i>, and type 1851 and 1701 filamentous bacteria. A greater abundance of bacteria led to a faster-growing M-BGS biomass (to a level of 4800 ± 503 mgTS/dm<sup>3</sup>), as well as improved TOC and COD removal from the LF-DSS (88.2 ± 7.2% and 84.1 ± 5.1%). The efficiency of N/P removal was comparable, since regardless of the composition and concentration of biomass, it ranged from 68.9 ± 3.1% to 71.3 ± 3.1% for N and from 54.2 ± 4.1% to 56, 2 ± 4.6% for P. As the M-BGS taxonomic structure evolved and the C/N ratio improved, so did the anaerobic digestion (AD) performance. Biogas yield from the M-BGS peaked at 531 ± 38 cm<sup>3</sup>/gVS (methane fraction = 66.2 ± 2.7%). It was found that final effects of AD were also strongly correlated with the N and TOC content in the substrate and pH value. A mature M-BGS significantly improved settleability and separability through filtration. |
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| spelling | doaj.art-225d618bc4ab4bbd8c6ddb79c23da69f2023-11-30T21:06:10ZengMDPI AGApplied Sciences2076-34172023-01-01132109810.3390/app13021098Taxonomic Structure Evolution, Chemical Composition and Anaerobic Digestibility of Microalgae-Bacterial Granular Sludge (M-BGS) Grown during Treatment of DigestateJoanna Kazimierowicz0Marcin Dębowski1Marcin Zieliński2Department of Water Supply and Sewage Systems, Faculty of Civil Engineering and Environmental Sciences, Bialystok University of Technology, 15-351 Bialystok, PolandDepartment of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, PolandDepartment of Environment Engineering, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, PolandThe liquid fraction from the dewatering of digested sewage sludge (LF-DSS) represents a major processing complication for wastewater treatment facilities, thus necessitating new and effective methods of LF-DSS neutralization. This pilot-scale study examined the evolution of a <i>Chlorella</i> sp. monoculture into microalgal-bacterial granular sludge (M-BGS) during treatment of LF-DSS in a hybrid photo-bioreactor (H-PBR). The M-BGS reached a stable taxonomic and morphological structure after 60 days of H-PBR operation. The biomass was primarily composed of <i>Chlorella</i> sp., <i>Microthrix parvicella</i>, and type 1851 and 1701 filamentous bacteria. A greater abundance of bacteria led to a faster-growing M-BGS biomass (to a level of 4800 ± 503 mgTS/dm<sup>3</sup>), as well as improved TOC and COD removal from the LF-DSS (88.2 ± 7.2% and 84.1 ± 5.1%). The efficiency of N/P removal was comparable, since regardless of the composition and concentration of biomass, it ranged from 68.9 ± 3.1% to 71.3 ± 3.1% for N and from 54.2 ± 4.1% to 56, 2 ± 4.6% for P. As the M-BGS taxonomic structure evolved and the C/N ratio improved, so did the anaerobic digestion (AD) performance. Biogas yield from the M-BGS peaked at 531 ± 38 cm<sup>3</sup>/gVS (methane fraction = 66.2 ± 2.7%). It was found that final effects of AD were also strongly correlated with the N and TOC content in the substrate and pH value. A mature M-BGS significantly improved settleability and separability through filtration.https://www.mdpi.com/2076-3417/13/2/1098microalgal-bacterial consortiamicrobial granulesmicroalgae-bacterial granular sludgetaxonomic evolutiondigestate treatmentanaerobic digestion |
| spellingShingle | Joanna Kazimierowicz Marcin Dębowski Marcin Zieliński Taxonomic Structure Evolution, Chemical Composition and Anaerobic Digestibility of Microalgae-Bacterial Granular Sludge (M-BGS) Grown during Treatment of Digestate Applied Sciences microalgal-bacterial consortia microbial granules microalgae-bacterial granular sludge taxonomic evolution digestate treatment anaerobic digestion |
| title | Taxonomic Structure Evolution, Chemical Composition and Anaerobic Digestibility of Microalgae-Bacterial Granular Sludge (M-BGS) Grown during Treatment of Digestate |
| title_full | Taxonomic Structure Evolution, Chemical Composition and Anaerobic Digestibility of Microalgae-Bacterial Granular Sludge (M-BGS) Grown during Treatment of Digestate |
| title_fullStr | Taxonomic Structure Evolution, Chemical Composition and Anaerobic Digestibility of Microalgae-Bacterial Granular Sludge (M-BGS) Grown during Treatment of Digestate |
| title_full_unstemmed | Taxonomic Structure Evolution, Chemical Composition and Anaerobic Digestibility of Microalgae-Bacterial Granular Sludge (M-BGS) Grown during Treatment of Digestate |
| title_short | Taxonomic Structure Evolution, Chemical Composition and Anaerobic Digestibility of Microalgae-Bacterial Granular Sludge (M-BGS) Grown during Treatment of Digestate |
| title_sort | taxonomic structure evolution chemical composition and anaerobic digestibility of microalgae bacterial granular sludge m bgs grown during treatment of digestate |
| topic | microalgal-bacterial consortia microbial granules microalgae-bacterial granular sludge taxonomic evolution digestate treatment anaerobic digestion |
| url | https://www.mdpi.com/2076-3417/13/2/1098 |
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