Safety evaluation of Bon-santé cleanser® polyherbal in male Wistar rats: Further investigations on androgenic and toxicological profile
Background: The global increase in acceptance and use of herbal remedies in recent times is still accompanied with poor knowledge of their potential adverse effects and the toxicological implications of their use are underestimated. Methods: Bon-santé Cleanser® (BSC), a polyherbal containing Anogeis...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | Journal of Traditional and Complementary Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411017300640 |
| _version_ | 1818202510188347392 |
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| author | O. Awodele O.E. Kale A.O. Odewabi M. Ekor B.A. Salau A.O. Adefule-Ositelu |
| author_facet | O. Awodele O.E. Kale A.O. Odewabi M. Ekor B.A. Salau A.O. Adefule-Ositelu |
| author_sort | O. Awodele |
| collection | DOAJ |
| description | Background: The global increase in acceptance and use of herbal remedies in recent times is still accompanied with poor knowledge of their potential adverse effects and the toxicological implications of their use are underestimated.
Methods: Bon-santé Cleanser® (BSC), a polyherbal containing Anogeissus leiocarpus, Terminalia ivorensis, Massularia acuminate and Macuna pruriens, is an “energizer and hormone booster”. We assessed the effect of BSC on reproductive function after administration for 60 days in male Wistar rats. Rats (150–300 g) were assigned into four groups of 8/group. Control received distilled water (10 ml/kg) while other groups received BSC 250, 500 and 1000 mg/kg/day p.o. respectively. Animals were euthanized by cervical dislocation and samples collected for analysis.
Results: BSC (250 mg/kg) elevated (p < 0.05) follicle stimulating hormone and luteinizing hormone levels respectively. BSC decreased sperm motility and the live-dead ratio at 1000 mg/kg and reduced reproductive hormone at 500 mg/kg and 1000 mg/kg respectively. BSC at 500 mg/kg increased (p < 0.05, F = 3.18–13.21) testicular reduced glutathione level (50.3%) and catalase (43.7%) but not activities of superoxide dismutase, glutathione S-transferase, and malondialdehyde level. Further, BSC influenced Mg, Zn, Cu, P, Mn, Ni and Fe levels (p < 0.05). BSC (1000 mg/kg) decreased testis weight (p < 0.05) and induced mild inflammation characterized by atrophic tubules.
Conclusion: Overall, our data suggest BSC at low doses may increase reproductive hormones regulated by FSH and LH as observed in this study. However, BSC administration should be done with caution as it may induce reproductive toxicity in large doses. |
| first_indexed | 2024-12-12T03:10:35Z |
| format | Article |
| id | doaj.art-225f1de7119348fe9baa7c4e7759b0c7 |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-12-12T03:10:35Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-225f1de7119348fe9baa7c4e7759b0c72022-12-22T00:40:25ZengElsevierJournal of Traditional and Complementary Medicine2225-41102018-01-018121221910.1016/j.jtcme.2017.06.002Safety evaluation of Bon-santé cleanser® polyherbal in male Wistar rats: Further investigations on androgenic and toxicological profileO. Awodele0O.E. Kale1A.O. Odewabi2M. Ekor3B.A. Salau4A.O. Adefule-Ositelu5Department of Pharmacology, Therapeutics, and Toxicology, College of Medicine, University of Lagos, NigeriaDepartment of Pharmacology, Therapeutics, and Toxicology, College of Medicine, University of Lagos, NigeriaDepartment of Chemical Pathology, Olabisi Onabanjo University Teaching Hospital, NigeriaDepartment of Pharmacology, School of Medical Sciences, University of Cape Coast, GhanaDepartment of Chemical sciences, Redeemers University, Ede, Osun State, NigeriaGuinness Eye Center, Lagos University Teaching Hospital, Lagos State, NigeriaBackground: The global increase in acceptance and use of herbal remedies in recent times is still accompanied with poor knowledge of their potential adverse effects and the toxicological implications of their use are underestimated. Methods: Bon-santé Cleanser® (BSC), a polyherbal containing Anogeissus leiocarpus, Terminalia ivorensis, Massularia acuminate and Macuna pruriens, is an “energizer and hormone booster”. We assessed the effect of BSC on reproductive function after administration for 60 days in male Wistar rats. Rats (150–300 g) were assigned into four groups of 8/group. Control received distilled water (10 ml/kg) while other groups received BSC 250, 500 and 1000 mg/kg/day p.o. respectively. Animals were euthanized by cervical dislocation and samples collected for analysis. Results: BSC (250 mg/kg) elevated (p < 0.05) follicle stimulating hormone and luteinizing hormone levels respectively. BSC decreased sperm motility and the live-dead ratio at 1000 mg/kg and reduced reproductive hormone at 500 mg/kg and 1000 mg/kg respectively. BSC at 500 mg/kg increased (p < 0.05, F = 3.18–13.21) testicular reduced glutathione level (50.3%) and catalase (43.7%) but not activities of superoxide dismutase, glutathione S-transferase, and malondialdehyde level. Further, BSC influenced Mg, Zn, Cu, P, Mn, Ni and Fe levels (p < 0.05). BSC (1000 mg/kg) decreased testis weight (p < 0.05) and induced mild inflammation characterized by atrophic tubules. Conclusion: Overall, our data suggest BSC at low doses may increase reproductive hormones regulated by FSH and LH as observed in this study. However, BSC administration should be done with caution as it may induce reproductive toxicity in large doses.http://www.sciencedirect.com/science/article/pii/S2225411017300640Bon-santé Cleanser® capsuleMale reproductive functionBiological elementsHerbal capsuleToxicology |
| spellingShingle | O. Awodele O.E. Kale A.O. Odewabi M. Ekor B.A. Salau A.O. Adefule-Ositelu Safety evaluation of Bon-santé cleanser® polyherbal in male Wistar rats: Further investigations on androgenic and toxicological profile Journal of Traditional and Complementary Medicine Bon-santé Cleanser® capsule Male reproductive function Biological elements Herbal capsule Toxicology |
| title | Safety evaluation of Bon-santé cleanser® polyherbal in male Wistar rats: Further investigations on androgenic and toxicological profile |
| title_full | Safety evaluation of Bon-santé cleanser® polyherbal in male Wistar rats: Further investigations on androgenic and toxicological profile |
| title_fullStr | Safety evaluation of Bon-santé cleanser® polyherbal in male Wistar rats: Further investigations on androgenic and toxicological profile |
| title_full_unstemmed | Safety evaluation of Bon-santé cleanser® polyherbal in male Wistar rats: Further investigations on androgenic and toxicological profile |
| title_short | Safety evaluation of Bon-santé cleanser® polyherbal in male Wistar rats: Further investigations on androgenic and toxicological profile |
| title_sort | safety evaluation of bon sante cleanser r polyherbal in male wistar rats further investigations on androgenic and toxicological profile |
| topic | Bon-santé Cleanser® capsule Male reproductive function Biological elements Herbal capsule Toxicology |
| url | http://www.sciencedirect.com/science/article/pii/S2225411017300640 |
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