Genetic Diversity and Population Dynamics of <i>Leptobrachium leishanense</i> (Anura: Megophryidae) as Determined by Tetranucleotide Microsatellite Markers Developed from Its Genome
Persisting declination of amphibians around the world has resulted in the public attaching importance to the conservation of their biodiversity. Genetic data can be greatly helpful in conservation planning and management, especially in species that are small in size and hard to observe. It is essent...
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MDPI AG
2021-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/12/3560 |
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| author | Chao Fu Qingbo Ai Ling Cai Fuyuan Qiu Lei Yao Hua Wu |
| author_facet | Chao Fu Qingbo Ai Ling Cai Fuyuan Qiu Lei Yao Hua Wu |
| author_sort | Chao Fu |
| collection | DOAJ |
| description | Persisting declination of amphibians around the world has resulted in the public attaching importance to the conservation of their biodiversity. Genetic data can be greatly helpful in conservation planning and management, especially in species that are small in size and hard to observe. It is essential to perform genetic assessments for the conservation of <i>Leptobrachium leishanense</i>, an endangered toad and receiving secondary protection on the list of state-protected wildlife in China. However, current molecular markers with low reliability and efficiency hinder studies. Here, we sampled 120 adult toes from the population in the Leishan Mountain, 23 of which were used to develop tetranucleotide microsatellite markers based on one reference <i>L. leishanense</i> genome. After primer optimization, stability detection, and polymorphism detection, we obtained 12 satisfactory microsatellite loci. Then, we used these loci to evaluate the genetic diversity and population dynamics of the 120 individuals. Our results show that there is a low degree of inbreeding in the population, and it has a high genetic diversity. Recently, the population has not experienced population bottlenecks, and the estimated effective population size was 424.3. Accordingly, stabilizing genetic diversity will be key to population sustainability. Recovering its habitat and avoiding intentional human use will be useful for conservation of this species. |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T04:39:17Z |
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| spelling | doaj.art-225f45e7b1e848488d2f88cf7fd449d72023-11-23T03:28:53ZengMDPI AGAnimals2076-26152021-12-011112356010.3390/ani11123560Genetic Diversity and Population Dynamics of <i>Leptobrachium leishanense</i> (Anura: Megophryidae) as Determined by Tetranucleotide Microsatellite Markers Developed from Its GenomeChao Fu0Qingbo Ai1Ling Cai2Fuyuan Qiu3Lei Yao4Hua Wu5Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, ChinaInstitute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, ChinaInstitute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, ChinaInstitute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, ChinaInstitute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, ChinaInstitute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, ChinaPersisting declination of amphibians around the world has resulted in the public attaching importance to the conservation of their biodiversity. Genetic data can be greatly helpful in conservation planning and management, especially in species that are small in size and hard to observe. It is essential to perform genetic assessments for the conservation of <i>Leptobrachium leishanense</i>, an endangered toad and receiving secondary protection on the list of state-protected wildlife in China. However, current molecular markers with low reliability and efficiency hinder studies. Here, we sampled 120 adult toes from the population in the Leishan Mountain, 23 of which were used to develop tetranucleotide microsatellite markers based on one reference <i>L. leishanense</i> genome. After primer optimization, stability detection, and polymorphism detection, we obtained 12 satisfactory microsatellite loci. Then, we used these loci to evaluate the genetic diversity and population dynamics of the 120 individuals. Our results show that there is a low degree of inbreeding in the population, and it has a high genetic diversity. Recently, the population has not experienced population bottlenecks, and the estimated effective population size was 424.3. Accordingly, stabilizing genetic diversity will be key to population sustainability. Recovering its habitat and avoiding intentional human use will be useful for conservation of this species.https://www.mdpi.com/2076-2615/11/12/3560Chinese endemic froggenetic diversitymicrosatellite markerspopulation dynamicswildlife conservation |
| spellingShingle | Chao Fu Qingbo Ai Ling Cai Fuyuan Qiu Lei Yao Hua Wu Genetic Diversity and Population Dynamics of <i>Leptobrachium leishanense</i> (Anura: Megophryidae) as Determined by Tetranucleotide Microsatellite Markers Developed from Its Genome Animals Chinese endemic frog genetic diversity microsatellite markers population dynamics wildlife conservation |
| title | Genetic Diversity and Population Dynamics of <i>Leptobrachium leishanense</i> (Anura: Megophryidae) as Determined by Tetranucleotide Microsatellite Markers Developed from Its Genome |
| title_full | Genetic Diversity and Population Dynamics of <i>Leptobrachium leishanense</i> (Anura: Megophryidae) as Determined by Tetranucleotide Microsatellite Markers Developed from Its Genome |
| title_fullStr | Genetic Diversity and Population Dynamics of <i>Leptobrachium leishanense</i> (Anura: Megophryidae) as Determined by Tetranucleotide Microsatellite Markers Developed from Its Genome |
| title_full_unstemmed | Genetic Diversity and Population Dynamics of <i>Leptobrachium leishanense</i> (Anura: Megophryidae) as Determined by Tetranucleotide Microsatellite Markers Developed from Its Genome |
| title_short | Genetic Diversity and Population Dynamics of <i>Leptobrachium leishanense</i> (Anura: Megophryidae) as Determined by Tetranucleotide Microsatellite Markers Developed from Its Genome |
| title_sort | genetic diversity and population dynamics of i leptobrachium leishanense i anura megophryidae as determined by tetranucleotide microsatellite markers developed from its genome |
| topic | Chinese endemic frog genetic diversity microsatellite markers population dynamics wildlife conservation |
| url | https://www.mdpi.com/2076-2615/11/12/3560 |
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