An In Vitro and In Silico Investigation about <i>Monteverdia ilicifolia</i> Activity against <i>Helicobacter pylori</i>
<i>Monteverdia ilicifolia</i> is a Brazilian native plant, traditionally used to treat gastric diseases that are now associated with <i>Helicobacter pylori</i> and are commonly associated with several human diseases. We point out the <i>M. ilicifolia</i> extract a...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/12/1/46 |
| _version_ | 1797446947168059392 |
|---|---|
| author | Mariana Nascimento de Paula Taísa Dalla Valle Rörig Ribeiro Raquel Isolani Daniela Cristina de Medeiros Araújo Augusto Santos Borges Gisele Strieder Philippsen Rita de Cássia Ribeiro Gonçalves Rodrigo Rezende Kitagawa Flavio Augusto Vicente Seixas João Carlos Palazzo de Mello |
| author_facet | Mariana Nascimento de Paula Taísa Dalla Valle Rörig Ribeiro Raquel Isolani Daniela Cristina de Medeiros Araújo Augusto Santos Borges Gisele Strieder Philippsen Rita de Cássia Ribeiro Gonçalves Rodrigo Rezende Kitagawa Flavio Augusto Vicente Seixas João Carlos Palazzo de Mello |
| author_sort | Mariana Nascimento de Paula |
| collection | DOAJ |
| description | <i>Monteverdia ilicifolia</i> is a Brazilian native plant, traditionally used to treat gastric diseases that are now associated with <i>Helicobacter pylori</i> and are commonly associated with several human diseases. We point out the <i>M. ilicifolia</i> extract as active against <i>H. pylori</i>. The crude extract produced with acetone:water presented the best <i>H. pylori</i> inhibitory activity of all five extracts (MIC 64 µg/mL). The ethyl-acetate fractions from crude extracts produced with ethanol and acetone showed a MIC of 64 µg/mL. Both ethyl-acetate fractions and the crude extract produced with acetone showed an antioxidant capacity of between 14.51 and 19.48 µg/mL in the DPPH assay. In the FRAP assay, two ethyl-acetate fractions (EAF2 and EAF4) presented the antioxidant capacity of 5.40 and 5.15 mM Trolox/g of extract. According to the results obtained from the antioxidant and antibacterial assays, two fractions (EAF2 and nBF5) were analyzed by mass spectrometry and confirmed the presence of monomeric, dimeric, trimeric tannins, and glycosylated flavonoids. Some compounds were tested using bioinformatics to evaluate the best enzyme inhibitors and the molecular interaction between the enzyme and the tested ligands. The presence of these polyphenol compounds could play an important role in antioxidant and inhibitory capacities against <i>H. pylori</i> and can be used to assist in the treatment or prevention of infection by <i>H. pylori.</i> |
| first_indexed | 2024-03-09T13:47:51Z |
| format | Article |
| id | doaj.art-2261f744723c475a99d6f76fd3c032d1 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T13:47:51Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-2261f744723c475a99d6f76fd3c032d12023-11-30T20:54:57ZengMDPI AGAntibiotics2079-63822022-12-011214610.3390/antibiotics12010046An In Vitro and In Silico Investigation about <i>Monteverdia ilicifolia</i> Activity against <i>Helicobacter pylori</i>Mariana Nascimento de Paula0Taísa Dalla Valle Rörig Ribeiro1Raquel Isolani2Daniela Cristina de Medeiros Araújo3Augusto Santos Borges4Gisele Strieder Philippsen5Rita de Cássia Ribeiro Gonçalves6Rodrigo Rezende Kitagawa7Flavio Augusto Vicente Seixas8João Carlos Palazzo de Mello9Laboratory of Pharmaceutical Biology, Department of Pharmacy, State University of Maringá, Maringá 87020-900, BrazilLaboratory of Pharmaceutical Biology, Department of Pharmacy, State University of Maringá, Maringá 87020-900, BrazilLaboratory of Pharmaceutical Biology, Department of Pharmacy, State University of Maringá, Maringá 87020-900, BrazilIngá University Center, Maringá 87035-510, BrazilDepartment of Pharmaceutical Science, Federal University of Espirito Santo, Vitória 29047-105, BrazilFederal University of Paraná, Jandaia do Sul 86900-000, BrazilDepartment of Pharmaceutical Science, Federal University of Espirito Santo, Vitória 29047-105, BrazilDepartment of Pharmaceutical Science, Federal University of Espirito Santo, Vitória 29047-105, BrazilDepartment of Technology, State University of Maringá, Umuarama 87501-390, BrazilLaboratory of Pharmaceutical Biology, Department of Pharmacy, State University of Maringá, Maringá 87020-900, Brazil<i>Monteverdia ilicifolia</i> is a Brazilian native plant, traditionally used to treat gastric diseases that are now associated with <i>Helicobacter pylori</i> and are commonly associated with several human diseases. We point out the <i>M. ilicifolia</i> extract as active against <i>H. pylori</i>. The crude extract produced with acetone:water presented the best <i>H. pylori</i> inhibitory activity of all five extracts (MIC 64 µg/mL). The ethyl-acetate fractions from crude extracts produced with ethanol and acetone showed a MIC of 64 µg/mL. Both ethyl-acetate fractions and the crude extract produced with acetone showed an antioxidant capacity of between 14.51 and 19.48 µg/mL in the DPPH assay. In the FRAP assay, two ethyl-acetate fractions (EAF2 and EAF4) presented the antioxidant capacity of 5.40 and 5.15 mM Trolox/g of extract. According to the results obtained from the antioxidant and antibacterial assays, two fractions (EAF2 and nBF5) were analyzed by mass spectrometry and confirmed the presence of monomeric, dimeric, trimeric tannins, and glycosylated flavonoids. Some compounds were tested using bioinformatics to evaluate the best enzyme inhibitors and the molecular interaction between the enzyme and the tested ligands. The presence of these polyphenol compounds could play an important role in antioxidant and inhibitory capacities against <i>H. pylori</i> and can be used to assist in the treatment or prevention of infection by <i>H. pylori.</i>https://www.mdpi.com/2079-6382/12/1/46<i>Monteverdia ilicifolia</i>Maytenus ilicifoliaantioxidant capacitycondensed tanninsglycosylated flavonoidsbioinformatics |
| spellingShingle | Mariana Nascimento de Paula Taísa Dalla Valle Rörig Ribeiro Raquel Isolani Daniela Cristina de Medeiros Araújo Augusto Santos Borges Gisele Strieder Philippsen Rita de Cássia Ribeiro Gonçalves Rodrigo Rezende Kitagawa Flavio Augusto Vicente Seixas João Carlos Palazzo de Mello An In Vitro and In Silico Investigation about <i>Monteverdia ilicifolia</i> Activity against <i>Helicobacter pylori</i> Antibiotics <i>Monteverdia ilicifolia</i> Maytenus ilicifolia antioxidant capacity condensed tannins glycosylated flavonoids bioinformatics |
| title | An In Vitro and In Silico Investigation about <i>Monteverdia ilicifolia</i> Activity against <i>Helicobacter pylori</i> |
| title_full | An In Vitro and In Silico Investigation about <i>Monteverdia ilicifolia</i> Activity against <i>Helicobacter pylori</i> |
| title_fullStr | An In Vitro and In Silico Investigation about <i>Monteverdia ilicifolia</i> Activity against <i>Helicobacter pylori</i> |
| title_full_unstemmed | An In Vitro and In Silico Investigation about <i>Monteverdia ilicifolia</i> Activity against <i>Helicobacter pylori</i> |
| title_short | An In Vitro and In Silico Investigation about <i>Monteverdia ilicifolia</i> Activity against <i>Helicobacter pylori</i> |
| title_sort | in vitro and in silico investigation about i monteverdia ilicifolia i activity against i helicobacter pylori i |
| topic | <i>Monteverdia ilicifolia</i> Maytenus ilicifolia antioxidant capacity condensed tannins glycosylated flavonoids bioinformatics |
| url | https://www.mdpi.com/2079-6382/12/1/46 |
| work_keys_str_mv | AT mariananascimentodepaula aninvitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT taisadallavallerorigribeiro aninvitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT raquelisolani aninvitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT danielacristinademedeirosaraujo aninvitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT augustosantosborges aninvitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT giselestriederphilippsen aninvitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT ritadecassiaribeirogoncalves aninvitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT rodrigorezendekitagawa aninvitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT flavioaugustovicenteseixas aninvitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT joaocarlospalazzodemello aninvitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT mariananascimentodepaula invitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT taisadallavallerorigribeiro invitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT raquelisolani invitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT danielacristinademedeirosaraujo invitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT augustosantosborges invitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT giselestriederphilippsen invitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT ritadecassiaribeirogoncalves invitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT rodrigorezendekitagawa invitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT flavioaugustovicenteseixas invitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii AT joaocarlospalazzodemello invitroandinsilicoinvestigationaboutimonteverdiailicifoliaiactivityagainstihelicobacterpylorii |