Phenolics Profiling by HPLC-DAD-ESI/MS<sup>n</sup> of the Scientific Unknown <i>Polygonum hydropiperoides</i> Michx. and Its Antioxidant and Anti-Methicillin-Resistant <i>Staphylococcus aureus</i> Activities
<i>Polygonum hydropiperoides</i> Michx. is an Asian native plant species that is also widely distributed in the Americas. Despite its traditional uses, <i>P. hydropiperoides</i> is scarcely scientifically exploited. This study aimed to chemically characterize and investigate...
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MDPI AG
2023-04-01
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| author | Ygor Ferreira Garcia da Costa Eulogio José Llorent-Martínez Laura Silva Fernandes Pedro Henrique Santos de Freitas Elita Scio Orlando Vieira de Sousa Paula Cristina Castilho Maria Silvana Alves |
| author_facet | Ygor Ferreira Garcia da Costa Eulogio José Llorent-Martínez Laura Silva Fernandes Pedro Henrique Santos de Freitas Elita Scio Orlando Vieira de Sousa Paula Cristina Castilho Maria Silvana Alves |
| author_sort | Ygor Ferreira Garcia da Costa |
| collection | DOAJ |
| description | <i>Polygonum hydropiperoides</i> Michx. is an Asian native plant species that is also widely distributed in the Americas. Despite its traditional uses, <i>P. hydropiperoides</i> is scarcely scientifically exploited. This study aimed to chemically characterize and investigate the antioxidant and antibacterial activities of hexane (HE-Ph), ethyl acetate (EAE-Ph), and ethanolic (EE-Ph) extracts from aerial parts of <i>P. hydropiperoides</i>. The chemical characterization was performed through HPLC-DAD-ESI/MS<sup>n</sup>. The antioxidant activity was assessed by the phosphomolybdenum reducing power, nitric oxide inhibition, and the β-carotene bleaching assays. The antibacterial activity was determined by the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration followed by the classification of the antibacterial effect. Chemical characterization revealed the expressive presence of phenolic acids and flavonoids in EAE-Ph. An increased antioxidant capacity was revealed in EAE-Ph. Regarding antibacterial activity, EAE-Ph showed weak to moderate property against 13 strains tested with MIC values ranging from 625 to 5000 µg/mL, with bactericidal or bacteriostatic effects. Glucogallin and gallic acid stand out as the most relevant bioactive compounds. These results suggest that <i>P. hydropiperoides</i> is a natural source of active substances, supporting this species’ traditional use. |
| first_indexed | 2024-03-11T04:37:02Z |
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| id | doaj.art-2262305652fb431f953853d419cf9b05 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T04:37:02Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-2262305652fb431f953853d419cf9b052023-11-17T20:59:04ZengMDPI AGPlants2223-77472023-04-01128160610.3390/plants12081606Phenolics Profiling by HPLC-DAD-ESI/MS<sup>n</sup> of the Scientific Unknown <i>Polygonum hydropiperoides</i> Michx. and Its Antioxidant and Anti-Methicillin-Resistant <i>Staphylococcus aureus</i> ActivitiesYgor Ferreira Garcia da Costa0Eulogio José Llorent-Martínez1Laura Silva Fernandes2Pedro Henrique Santos de Freitas3Elita Scio4Orlando Vieira de Sousa5Paula Cristina Castilho6Maria Silvana Alves7Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora 36036-900, BrazilDepartment of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Campus Las Lagunillas s/n, E-23071 Jaén, SpainDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora 36036-900, BrazilDepartment of Biochemistry, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora 36036-900, BrazilDepartment of Biochemistry, Biological Sciences Institute, Federal University of Juiz de Fora, Juiz de Fora 36036-900, BrazilDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora 36036-900, BrazilMadeira Chemical Center, Faculty of Exact and Engineering, University of Madeira, Campus da Penteada, 9020-105 Funchal, PortugalDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora 36036-900, Brazil<i>Polygonum hydropiperoides</i> Michx. is an Asian native plant species that is also widely distributed in the Americas. Despite its traditional uses, <i>P. hydropiperoides</i> is scarcely scientifically exploited. This study aimed to chemically characterize and investigate the antioxidant and antibacterial activities of hexane (HE-Ph), ethyl acetate (EAE-Ph), and ethanolic (EE-Ph) extracts from aerial parts of <i>P. hydropiperoides</i>. The chemical characterization was performed through HPLC-DAD-ESI/MS<sup>n</sup>. The antioxidant activity was assessed by the phosphomolybdenum reducing power, nitric oxide inhibition, and the β-carotene bleaching assays. The antibacterial activity was determined by the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration followed by the classification of the antibacterial effect. Chemical characterization revealed the expressive presence of phenolic acids and flavonoids in EAE-Ph. An increased antioxidant capacity was revealed in EAE-Ph. Regarding antibacterial activity, EAE-Ph showed weak to moderate property against 13 strains tested with MIC values ranging from 625 to 5000 µg/mL, with bactericidal or bacteriostatic effects. Glucogallin and gallic acid stand out as the most relevant bioactive compounds. These results suggest that <i>P. hydropiperoides</i> is a natural source of active substances, supporting this species’ traditional use.https://www.mdpi.com/2223-7747/12/8/1606<i>Polygonum hydropiperoides</i>gallic acid derivativesflavonoidsantioxidantsantibacterial agentsmethicillin-resistant <i>Staphylococcus aureus</i> |
| spellingShingle | Ygor Ferreira Garcia da Costa Eulogio José Llorent-Martínez Laura Silva Fernandes Pedro Henrique Santos de Freitas Elita Scio Orlando Vieira de Sousa Paula Cristina Castilho Maria Silvana Alves Phenolics Profiling by HPLC-DAD-ESI/MS<sup>n</sup> of the Scientific Unknown <i>Polygonum hydropiperoides</i> Michx. and Its Antioxidant and Anti-Methicillin-Resistant <i>Staphylococcus aureus</i> Activities Plants <i>Polygonum hydropiperoides</i> gallic acid derivatives flavonoids antioxidants antibacterial agents methicillin-resistant <i>Staphylococcus aureus</i> |
| title | Phenolics Profiling by HPLC-DAD-ESI/MS<sup>n</sup> of the Scientific Unknown <i>Polygonum hydropiperoides</i> Michx. and Its Antioxidant and Anti-Methicillin-Resistant <i>Staphylococcus aureus</i> Activities |
| title_full | Phenolics Profiling by HPLC-DAD-ESI/MS<sup>n</sup> of the Scientific Unknown <i>Polygonum hydropiperoides</i> Michx. and Its Antioxidant and Anti-Methicillin-Resistant <i>Staphylococcus aureus</i> Activities |
| title_fullStr | Phenolics Profiling by HPLC-DAD-ESI/MS<sup>n</sup> of the Scientific Unknown <i>Polygonum hydropiperoides</i> Michx. and Its Antioxidant and Anti-Methicillin-Resistant <i>Staphylococcus aureus</i> Activities |
| title_full_unstemmed | Phenolics Profiling by HPLC-DAD-ESI/MS<sup>n</sup> of the Scientific Unknown <i>Polygonum hydropiperoides</i> Michx. and Its Antioxidant and Anti-Methicillin-Resistant <i>Staphylococcus aureus</i> Activities |
| title_short | Phenolics Profiling by HPLC-DAD-ESI/MS<sup>n</sup> of the Scientific Unknown <i>Polygonum hydropiperoides</i> Michx. and Its Antioxidant and Anti-Methicillin-Resistant <i>Staphylococcus aureus</i> Activities |
| title_sort | phenolics profiling by hplc dad esi ms sup n sup of the scientific unknown i polygonum hydropiperoides i michx and its antioxidant and anti methicillin resistant i staphylococcus aureus i activities |
| topic | <i>Polygonum hydropiperoides</i> gallic acid derivatives flavonoids antioxidants antibacterial agents methicillin-resistant <i>Staphylococcus aureus</i> |
| url | https://www.mdpi.com/2223-7747/12/8/1606 |
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