Bees can be trained to identify SARS-CoV-2 infected samples
The COVID-19 pandemic has illustrated the need for the development of fast and reliable testing methods for novel, zoonotic, viral diseases in both humans and animals. Pathologies lead to detectable changes in the volatile organic compound (VOC) profile of animals, which can be monitored, thus allow...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2022-04-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/11/4/bio059111 |
| _version_ | 1828816642327445504 |
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| author | Evangelos Kontos Aria Samimi Renate W. Hakze–van der Honing Jan Priem Aurore Avarguès-Weber Alexander Haverkamp Marcel Dicke Jose L. Gonzales Wim H. M. van der Poel |
| author_facet | Evangelos Kontos Aria Samimi Renate W. Hakze–van der Honing Jan Priem Aurore Avarguès-Weber Alexander Haverkamp Marcel Dicke Jose L. Gonzales Wim H. M. van der Poel |
| author_sort | Evangelos Kontos |
| collection | DOAJ |
| description | The COVID-19 pandemic has illustrated the need for the development of fast and reliable testing methods for novel, zoonotic, viral diseases in both humans and animals. Pathologies lead to detectable changes in the volatile organic compound (VOC) profile of animals, which can be monitored, thus allowing the development of a rapid VOC-based test. In the current study, we successfully trained honeybees (Apis mellifera) to identify SARS-CoV-2 infected minks (Neovison vison) thanks to Pavlovian conditioning protocols. The bees can be quickly conditioned to respond specifically to infected mink's odours and could therefore be part of a wider SARS-CoV-2 diagnostic system. We tested two different training protocols to evaluate their performance in terms of learning rate, accuracy and memory retention. We designed a non-invasive rapid test in which multiple bees are tested in parallel on the same samples. This provided reliable results regarding a subject's health status. Using the data from the training experiments, we simulated a diagnostic evaluation trial to predict the potential efficacy of our diagnostic test, which yielded a diagnostic sensitivity of 92% and specificity of 86%. We suggest that a honeybee-based diagnostics can offer a reliable and rapid test that provides a readily available, low-input addition to the currently available testing methods. A honeybee-based diagnostic test might be particularly relevant for remote and developing communities that lack the resources and infrastructure required for mainstream testing methods. |
| first_indexed | 2024-12-12T11:07:58Z |
| format | Article |
| id | doaj.art-22676a88872a43578bbcbcce2f5ce646 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-12T11:07:58Z |
| publishDate | 2022-04-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-22676a88872a43578bbcbcce2f5ce6462022-12-22T00:26:22ZengThe Company of BiologistsBiology Open2046-63902022-04-0111410.1242/bio.059111059111Bees can be trained to identify SARS-CoV-2 infected samplesEvangelos Kontos0Aria Samimi1Renate W. Hakze–van der Honing2Jan Priem3Aurore Avarguès-Weber4Alexander Haverkamp5Marcel Dicke6Jose L. Gonzales7Wim H. M. van der Poel8 InsectSense, Plus Ultra-II Building, Bronland, 10, 6708 WH, Wageningen, The Netherlands InsectSense, Plus Ultra-II Building, Bronland, 10, 6708 WH, Wageningen, The Netherlands Wageningen Bioveterinary Research, P.O. Box 65, 8200 AB, Lelystad, The Netherlands Wageningen Bioveterinary Research, P.O. Box 65, 8200 AB, Lelystad, The Netherlands Centre de Recherches sur la Cognition Animale (CRCA), Centre de Biologie Intégrative (CBI), Université de Toulouse; CNRS, UPS, 118 Route de Narbonne, 31062 Toulouse, France Laboratory of Entomology, P.O. Box 16, 6700 AA, Wageningen, The Netherlands Laboratory of Entomology, P.O. Box 16, 6700 AA, Wageningen, The Netherlands Wageningen Bioveterinary Research, P.O. Box 65, 8200 AB, Lelystad, The Netherlands Wageningen Bioveterinary Research, P.O. Box 65, 8200 AB, Lelystad, The Netherlands The COVID-19 pandemic has illustrated the need for the development of fast and reliable testing methods for novel, zoonotic, viral diseases in both humans and animals. Pathologies lead to detectable changes in the volatile organic compound (VOC) profile of animals, which can be monitored, thus allowing the development of a rapid VOC-based test. In the current study, we successfully trained honeybees (Apis mellifera) to identify SARS-CoV-2 infected minks (Neovison vison) thanks to Pavlovian conditioning protocols. The bees can be quickly conditioned to respond specifically to infected mink's odours and could therefore be part of a wider SARS-CoV-2 diagnostic system. We tested two different training protocols to evaluate their performance in terms of learning rate, accuracy and memory retention. We designed a non-invasive rapid test in which multiple bees are tested in parallel on the same samples. This provided reliable results regarding a subject's health status. Using the data from the training experiments, we simulated a diagnostic evaluation trial to predict the potential efficacy of our diagnostic test, which yielded a diagnostic sensitivity of 92% and specificity of 86%. We suggest that a honeybee-based diagnostics can offer a reliable and rapid test that provides a readily available, low-input addition to the currently available testing methods. A honeybee-based diagnostic test might be particularly relevant for remote and developing communities that lack the resources and infrastructure required for mainstream testing methods.http://bio.biologists.org/content/11/4/bio059111honeybeesolfactionsars-cov2covid-19conditioningdetection |
| spellingShingle | Evangelos Kontos Aria Samimi Renate W. Hakze–van der Honing Jan Priem Aurore Avarguès-Weber Alexander Haverkamp Marcel Dicke Jose L. Gonzales Wim H. M. van der Poel Bees can be trained to identify SARS-CoV-2 infected samples Biology Open honeybees olfaction sars-cov2 covid-19 conditioning detection |
| title | Bees can be trained to identify SARS-CoV-2 infected samples |
| title_full | Bees can be trained to identify SARS-CoV-2 infected samples |
| title_fullStr | Bees can be trained to identify SARS-CoV-2 infected samples |
| title_full_unstemmed | Bees can be trained to identify SARS-CoV-2 infected samples |
| title_short | Bees can be trained to identify SARS-CoV-2 infected samples |
| title_sort | bees can be trained to identify sars cov 2 infected samples |
| topic | honeybees olfaction sars-cov2 covid-19 conditioning detection |
| url | http://bio.biologists.org/content/11/4/bio059111 |
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