Metabolic Mechanism of Plant Defense against Rice Blast Induced by Probenazole
The probenazole fungicide is used for controlling rice blast (<i>Magnaporthe grisea</i>) primarily by inducing disease resistance of the plant. To investigate the mechanism of induced plant defense, rice seedlings were treated with probenazole at 15 days post emergence, and non-treated p...
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MDPI AG
2021-04-01
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| author | Zhaochen Wu Guozhen Wang Borui Zhang Tan Dai Anyu Gu Xiaolin Li Xingkai Cheng Pengfei Liu Jianjun Hao Xili Liu |
| author_facet | Zhaochen Wu Guozhen Wang Borui Zhang Tan Dai Anyu Gu Xiaolin Li Xingkai Cheng Pengfei Liu Jianjun Hao Xili Liu |
| author_sort | Zhaochen Wu |
| collection | DOAJ |
| description | The probenazole fungicide is used for controlling rice blast (<i>Magnaporthe grisea</i>) primarily by inducing disease resistance of the plant. To investigate the mechanism of induced plant defense, rice seedlings were treated with probenazole at 15 days post emergence, and non-treated plants were used for the control. The plants were infected with <i>M. grisea</i> 5 days after chemical treatment and incubated in a greenhouse. After 7 days, rice seedlings were sampled. The metabolome of rice seedlings was chemically extracted and analyzed using gas chromatography and mass spectrum (GC-MS). The GC-MS data were processed using analysis of variance (ANOVA), principal component analysis (PCA) and metabolic pathway elucidation. Results showed that probenazole application significantly affected the metabolic profile of rice seedlings, and the effect was proportionally leveraged with the increase of probenazole concentration. Probenazole resulted in a change of 54 metabolites. Salicylic acid, γ-aminobutyrate, shikimate and several other primary metabolites related to plant resistance were significantly up-regulated and some metabolites such as phenylalanine, valine and proline were down-regulated in probenazole-treated seedlings. These results revealed a metabolic pathway of rice seedlings induced by probenazole treatment regarding the resistance to <i>M. grisea</i> infection. |
| first_indexed | 2024-03-10T12:16:09Z |
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| id | doaj.art-22677fc6075d4acd9f76a2c6c5aef268 |
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| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T12:16:09Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-22677fc6075d4acd9f76a2c6c5aef2682023-11-21T15:49:40ZengMDPI AGMetabolites2218-19892021-04-0111424610.3390/metabo11040246Metabolic Mechanism of Plant Defense against Rice Blast Induced by ProbenazoleZhaochen Wu0Guozhen Wang1Borui Zhang2Tan Dai3Anyu Gu4Xiaolin Li5Xingkai Cheng6Pengfei Liu7Jianjun Hao8Xili Liu9College of Plant Protection, China Agricultural University, Beijing 100193, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaInstitute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming 650205, ChinaInstitute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming 650205, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaCollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaSchool of Food and Agriculture, University of Maine, Orono, ME 04469, USACollege of Plant Protection, China Agricultural University, Beijing 100193, ChinaThe probenazole fungicide is used for controlling rice blast (<i>Magnaporthe grisea</i>) primarily by inducing disease resistance of the plant. To investigate the mechanism of induced plant defense, rice seedlings were treated with probenazole at 15 days post emergence, and non-treated plants were used for the control. The plants were infected with <i>M. grisea</i> 5 days after chemical treatment and incubated in a greenhouse. After 7 days, rice seedlings were sampled. The metabolome of rice seedlings was chemically extracted and analyzed using gas chromatography and mass spectrum (GC-MS). The GC-MS data were processed using analysis of variance (ANOVA), principal component analysis (PCA) and metabolic pathway elucidation. Results showed that probenazole application significantly affected the metabolic profile of rice seedlings, and the effect was proportionally leveraged with the increase of probenazole concentration. Probenazole resulted in a change of 54 metabolites. Salicylic acid, γ-aminobutyrate, shikimate and several other primary metabolites related to plant resistance were significantly up-regulated and some metabolites such as phenylalanine, valine and proline were down-regulated in probenazole-treated seedlings. These results revealed a metabolic pathway of rice seedlings induced by probenazole treatment regarding the resistance to <i>M. grisea</i> infection.https://www.mdpi.com/2218-1989/11/4/246probenazolemetabolomicssystemic acquired resistancesalicylic acid<i>Magnaporthe grisea</i>gas chromatography |
| spellingShingle | Zhaochen Wu Guozhen Wang Borui Zhang Tan Dai Anyu Gu Xiaolin Li Xingkai Cheng Pengfei Liu Jianjun Hao Xili Liu Metabolic Mechanism of Plant Defense against Rice Blast Induced by Probenazole Metabolites probenazole metabolomics systemic acquired resistance salicylic acid <i>Magnaporthe grisea</i> gas chromatography |
| title | Metabolic Mechanism of Plant Defense against Rice Blast Induced by Probenazole |
| title_full | Metabolic Mechanism of Plant Defense against Rice Blast Induced by Probenazole |
| title_fullStr | Metabolic Mechanism of Plant Defense against Rice Blast Induced by Probenazole |
| title_full_unstemmed | Metabolic Mechanism of Plant Defense against Rice Blast Induced by Probenazole |
| title_short | Metabolic Mechanism of Plant Defense against Rice Blast Induced by Probenazole |
| title_sort | metabolic mechanism of plant defense against rice blast induced by probenazole |
| topic | probenazole metabolomics systemic acquired resistance salicylic acid <i>Magnaporthe grisea</i> gas chromatography |
| url | https://www.mdpi.com/2218-1989/11/4/246 |
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