CLICK: one-step generation of conditional knockout mice

CLICK: one-step generation of conditional knockout mice

Abstract Background CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxP-flanked (floxed) alleles remain elusive. Results Here, we show that the electroporation of Cas9, two gRNAs, and long single-stranded DNA (lssDNA) into zygotes, termed CLICK (CRISPR...

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Bibliographic Details
Main Authors: Yoshiki Miyasaka, Yoshihiro Uno, Kazuto Yoshimi, Yayoi Kunihiro, Takuji Yoshimura, Tomohiro Tanaka, Harumi Ishikubo, Yuichi Hiraoka, Norihiko Takemoto, Takao Tanaka, Yoshihiro Ooguchi, Paul Skehel, Tomomi Aida, Junji Takeda, Tomoji Mashimo
Format: Article
Language:English
Published: BMC 2018-05-01
Series:BMC Genomics
Subjects:
CRISPR/Cas9
CLICK
Zygote electroporation
Long single-stranded DNA
Cre-loxP system
Online Access:http://link.springer.com/article/10.1186/s12864-018-4713-y

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