TRPA5 encodes a thermosensitive ankyrin ion channel receptor in a triatomine insect
Summary: As ectotherms, insects need heat-sensitive receptors to monitor environmental temperatures and facilitate thermoregulation. We show that TRPA5, a class of ankyrin transient receptor potential (TRP) channels absent in dipteran genomes, may function as insect heat receptors. In the triatomine...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224007636 |
| _version_ | 1797226608909615104 |
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| author | Marjorie A. Liénard David Baez-Nieto Cheng-Chia Tsai Wendy A. Valencia-Montoya Balder Werin Urban Johanson Jean-Marc Lassance Jen Q. Pan Nanfang Yu Naomi E. Pierce |
| author_facet | Marjorie A. Liénard David Baez-Nieto Cheng-Chia Tsai Wendy A. Valencia-Montoya Balder Werin Urban Johanson Jean-Marc Lassance Jen Q. Pan Nanfang Yu Naomi E. Pierce |
| author_sort | Marjorie A. Liénard |
| collection | DOAJ |
| description | Summary: As ectotherms, insects need heat-sensitive receptors to monitor environmental temperatures and facilitate thermoregulation. We show that TRPA5, a class of ankyrin transient receptor potential (TRP) channels absent in dipteran genomes, may function as insect heat receptors. In the triatomine bug Rhodnius prolixus (order: Hemiptera), a vector of Chagas disease, the channel RpTRPA5B displays a uniquely high thermosensitivity, with biophysical determinants including a large channel activation enthalpy change (72 kcal/mol), a high temperature coefficient (Q10 = 25), and in vitro temperature-induced currents from 53°C to 68°C (T0.5 = 58.6°C), similar to noxious TRPV receptors in mammals. Monomeric and tetrameric ion channel structure predictions show reliable parallels with fruit fly dTRPA1, with structural uniqueness in ankyrin repeat domains, the channel selectivity filter, and potential TRP functional modulator regions. Overall, the finding of a member of TRPA5 as a temperature-activated receptor illustrates the diversity of insect molecular heat detectors. |
| first_indexed | 2024-04-24T14:27:37Z |
| format | Article |
| id | doaj.art-2267e094972541aea03611109c7abb4d |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-24T14:27:37Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-2267e094972541aea03611109c7abb4d2024-04-03T04:27:14ZengElsevieriScience2589-00422024-04-01274109541TRPA5 encodes a thermosensitive ankyrin ion channel receptor in a triatomine insectMarjorie A. Liénard0David Baez-Nieto1Cheng-Chia Tsai2Wendy A. Valencia-Montoya3Balder Werin4Urban Johanson5Jean-Marc Lassance6Jen Q. Pan7Nanfang Yu8Naomi E. Pierce9Department of Biology, Lund University, 22362 Lund, Sweden; Department of Organismic and Evolutionary Biology and Museum of Comparative Zoology, Harvard University, Cambridge, MA 02138, USA; Broad Institute, Cambridge, MA 02142, USA; Corresponding authorStanley Center for Psychiatric Research, Broad Institute, Cambridge, MA 02142, USA; Corresponding authorDepartment of Applied Physics and Applied Mathematics, Columbia University, New York, NY 10027, USADepartment of Organismic and Evolutionary Biology and Museum of Comparative Zoology, Harvard University, Cambridge, MA 02138, USADivision of Biochemistry and Structural Biology, Department of Chemistry, Lund University, 22362 Lund, SwedenDivision of Biochemistry and Structural Biology, Department of Chemistry, Lund University, 22362 Lund, SwedenDepartment of Organismic and Evolutionary Biology and Museum of Comparative Zoology, Harvard University, Cambridge, MA 02138, USA; Laboratory of Evolutionary Neuroethology, GIGA Institute, University of Liège, 4000 Liège, BelgiumStanley Center for Psychiatric Research, Broad Institute, Cambridge, MA 02142, USADepartment of Applied Physics and Applied Mathematics, Columbia University, New York, NY 10027, USADepartment of Organismic and Evolutionary Biology and Museum of Comparative Zoology, Harvard University, Cambridge, MA 02138, USASummary: As ectotherms, insects need heat-sensitive receptors to monitor environmental temperatures and facilitate thermoregulation. We show that TRPA5, a class of ankyrin transient receptor potential (TRP) channels absent in dipteran genomes, may function as insect heat receptors. In the triatomine bug Rhodnius prolixus (order: Hemiptera), a vector of Chagas disease, the channel RpTRPA5B displays a uniquely high thermosensitivity, with biophysical determinants including a large channel activation enthalpy change (72 kcal/mol), a high temperature coefficient (Q10 = 25), and in vitro temperature-induced currents from 53°C to 68°C (T0.5 = 58.6°C), similar to noxious TRPV receptors in mammals. Monomeric and tetrameric ion channel structure predictions show reliable parallels with fruit fly dTRPA1, with structural uniqueness in ankyrin repeat domains, the channel selectivity filter, and potential TRP functional modulator regions. Overall, the finding of a member of TRPA5 as a temperature-activated receptor illustrates the diversity of insect molecular heat detectors.http://www.sciencedirect.com/science/article/pii/S2589004224007636Biological sciencesEntomologyMolecular biology |
| spellingShingle | Marjorie A. Liénard David Baez-Nieto Cheng-Chia Tsai Wendy A. Valencia-Montoya Balder Werin Urban Johanson Jean-Marc Lassance Jen Q. Pan Nanfang Yu Naomi E. Pierce TRPA5 encodes a thermosensitive ankyrin ion channel receptor in a triatomine insect iScience Biological sciences Entomology Molecular biology |
| title | TRPA5 encodes a thermosensitive ankyrin ion channel receptor in a triatomine insect |
| title_full | TRPA5 encodes a thermosensitive ankyrin ion channel receptor in a triatomine insect |
| title_fullStr | TRPA5 encodes a thermosensitive ankyrin ion channel receptor in a triatomine insect |
| title_full_unstemmed | TRPA5 encodes a thermosensitive ankyrin ion channel receptor in a triatomine insect |
| title_short | TRPA5 encodes a thermosensitive ankyrin ion channel receptor in a triatomine insect |
| title_sort | trpa5 encodes a thermosensitive ankyrin ion channel receptor in a triatomine insect |
| topic | Biological sciences Entomology Molecular biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224007636 |
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