Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation
Marine diatoms have recently gained much attention as they are expected to be a promising resource for sustainable production of bioactive compounds such as carotenoids and biofuels as a future clean energy solution. To develop photosynthetic cell factories, it is important to improve diatoms for va...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-09-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/13/10/6138 |
| _version_ | 1811301231459565568 |
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| author | Zhiqian Yi Maonian Xu Manuela Magnusdottir Yuetuan Zhang Sigurdur Brynjolfsson Weiqi Fu |
| author_facet | Zhiqian Yi Maonian Xu Manuela Magnusdottir Yuetuan Zhang Sigurdur Brynjolfsson Weiqi Fu |
| author_sort | Zhiqian Yi |
| collection | DOAJ |
| description | Marine diatoms have recently gained much attention as they are expected to be a promising resource for sustainable production of bioactive compounds such as carotenoids and biofuels as a future clean energy solution. To develop photosynthetic cell factories, it is important to improve diatoms for value-added products. In this study, we utilized UVC radiation to induce mutations in the marine diatom Phaeodactylum tricornutum and screened strains with enhanced accumulation of neutral lipids and carotenoids. Adaptive laboratory evolution (ALE) was also used in parallel to develop altered phenotypic and biological functions in P. tricornutum and it was reported for the first time that ALE was successfully applied on diatoms for the enhancement of growth performance and productivity of value-added carotenoids to date. Liquid chromatography-mass spectrometry (LC-MS) was utilized to study the composition of major pigments in the wild type P. tricornutum, UV mutants and ALE strains. UVC radiated strains exhibited higher accumulation of fucoxanthin as well as neutral lipids compared to their wild type counterpart. In addition to UV mutagenesis, P. tricornutum strains developed by ALE also yielded enhanced biomass production and fucoxanthin accumulation under combined red and blue light. In short, both UV mutagenesis and ALE appeared as an effective approach to developing desired phenotypes in the marine diatoms via electromagnetic radiation-induced oxidative stress. |
| first_indexed | 2024-04-13T07:05:11Z |
| format | Article |
| id | doaj.art-226914b764ba4701b8d67e2dfdb08d14 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-13T07:05:11Z |
| publishDate | 2015-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-226914b764ba4701b8d67e2dfdb08d142022-12-22T02:57:00ZengMDPI AGMarine Drugs1660-33972015-09-0113106138615110.3390/md13106138md13106138Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid AccumulationZhiqian Yi0Maonian Xu1Manuela Magnusdottir2Yuetuan Zhang3Sigurdur Brynjolfsson4Weiqi Fu5Center for Systems Biology and Faculty of Industrial Engineering, Mechanical Engineering and Computer Science, School of Engineering and Natural Sciences, University of Iceland, Reykjavík 101, IcelandFaculty of Pharmaceutical Sciences, University of Iceland, Hagi, Hofsvallagata 53, Reykjavik IS-107, IcelandCenter for Systems Biology and Faculty of Industrial Engineering, Mechanical Engineering and Computer Science, School of Engineering and Natural Sciences, University of Iceland, Reykjavík 101, IcelandCenter for Systems Biology and Faculty of Industrial Engineering, Mechanical Engineering and Computer Science, School of Engineering and Natural Sciences, University of Iceland, Reykjavík 101, IcelandCenter for Systems Biology and Faculty of Industrial Engineering, Mechanical Engineering and Computer Science, School of Engineering and Natural Sciences, University of Iceland, Reykjavík 101, IcelandCenter for Systems Biology and Faculty of Industrial Engineering, Mechanical Engineering and Computer Science, School of Engineering and Natural Sciences, University of Iceland, Reykjavík 101, IcelandMarine diatoms have recently gained much attention as they are expected to be a promising resource for sustainable production of bioactive compounds such as carotenoids and biofuels as a future clean energy solution. To develop photosynthetic cell factories, it is important to improve diatoms for value-added products. In this study, we utilized UVC radiation to induce mutations in the marine diatom Phaeodactylum tricornutum and screened strains with enhanced accumulation of neutral lipids and carotenoids. Adaptive laboratory evolution (ALE) was also used in parallel to develop altered phenotypic and biological functions in P. tricornutum and it was reported for the first time that ALE was successfully applied on diatoms for the enhancement of growth performance and productivity of value-added carotenoids to date. Liquid chromatography-mass spectrometry (LC-MS) was utilized to study the composition of major pigments in the wild type P. tricornutum, UV mutants and ALE strains. UVC radiated strains exhibited higher accumulation of fucoxanthin as well as neutral lipids compared to their wild type counterpart. In addition to UV mutagenesis, P. tricornutum strains developed by ALE also yielded enhanced biomass production and fucoxanthin accumulation under combined red and blue light. In short, both UV mutagenesis and ALE appeared as an effective approach to developing desired phenotypes in the marine diatoms via electromagnetic radiation-induced oxidative stress.http://www.mdpi.com/1660-3397/13/10/6138Phaeodactylum tricornutumUV mutagenesisadaptive laboratory evolution (ALE)fucoxanthinneutral lipids |
| spellingShingle | Zhiqian Yi Maonian Xu Manuela Magnusdottir Yuetuan Zhang Sigurdur Brynjolfsson Weiqi Fu Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation Marine Drugs Phaeodactylum tricornutum UV mutagenesis adaptive laboratory evolution (ALE) fucoxanthin neutral lipids |
| title | Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation |
| title_full | Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation |
| title_fullStr | Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation |
| title_full_unstemmed | Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation |
| title_short | Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation |
| title_sort | photo oxidative stress driven mutagenesis and adaptive evolution on the marine diatom phaeodactylum tricornutum for enhanced carotenoid accumulation |
| topic | Phaeodactylum tricornutum UV mutagenesis adaptive laboratory evolution (ALE) fucoxanthin neutral lipids |
| url | http://www.mdpi.com/1660-3397/13/10/6138 |
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