Duck plague virus US3 protein kinase phosphorylates UL47 and regulates the subcellular localization of UL47
Duck plague virus (DPV) belongs to the alphaherpesvirinae and causes high morbidity and mortality in waterfowl. UL47 is a large abundant structural protein in DPV, which means that UL47 protein plays an important role in virus replication. US3 protein, as a viral protein kinase in alphaherpesviruses...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.876820/full |
| _version_ | 1797985741859454976 |
|---|---|
| author | Liyao Deng Liyao Deng Liyao Deng Jieyu Wan Jieyu Wan Jieyu Wan Anchun Cheng Anchun Cheng Anchun Cheng Mingshu Wang Mingshu Wang Mingshu Wang Bin Tian Bin Tian Bin Tian Ying Wu Ying Wu Ying Wu Qiao Yang Qiao Yang Qiao Yang Xumin Ou Xumin Ou Xumin Ou Sai Mao Sai Mao Sai Mao Di Sun Di Sun Di Sun Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Dekang Zhu Dekang Zhu Renyong Jia Renyong Jia Renyong Jia Shun Chen Shun Chen Shun Chen Mafeng Liu Mafeng Liu Mafeng Liu Xinxin Zhao Xinxin Zhao Xinxin Zhao Juan Huang Juan Huang Juan Huang Qun Gao Qun Gao Qun Gao Yanling Yu Yanling Yu Yanling Yu Ling Zhang Ling Zhang Ling Zhang Leichang Pan Leichang Pan |
| author_facet | Liyao Deng Liyao Deng Liyao Deng Jieyu Wan Jieyu Wan Jieyu Wan Anchun Cheng Anchun Cheng Anchun Cheng Mingshu Wang Mingshu Wang Mingshu Wang Bin Tian Bin Tian Bin Tian Ying Wu Ying Wu Ying Wu Qiao Yang Qiao Yang Qiao Yang Xumin Ou Xumin Ou Xumin Ou Sai Mao Sai Mao Sai Mao Di Sun Di Sun Di Sun Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Dekang Zhu Dekang Zhu Renyong Jia Renyong Jia Renyong Jia Shun Chen Shun Chen Shun Chen Mafeng Liu Mafeng Liu Mafeng Liu Xinxin Zhao Xinxin Zhao Xinxin Zhao Juan Huang Juan Huang Juan Huang Qun Gao Qun Gao Qun Gao Yanling Yu Yanling Yu Yanling Yu Ling Zhang Ling Zhang Ling Zhang Leichang Pan Leichang Pan |
| author_sort | Liyao Deng |
| collection | DOAJ |
| description | Duck plague virus (DPV) belongs to the alphaherpesvirinae and causes high morbidity and mortality in waterfowl. UL47 is a large abundant structural protein in DPV, which means that UL47 protein plays an important role in virus replication. US3 protein, as a viral protein kinase in alphaherpesviruses, has been reported to be critical for DPV virion assembly. In this study, we over-expressed UL47 and US3 proteins and found that DPV UL47 protein was a phosphorylated substrate of US3 protein, which interacted and co-localized with US3 protein in the cytoplasm. US3-regulated phosphorylation of UL47 was important for the cytoplasmic localization of UL47 because non-phosphorylated UL47 was localized in the nucleus. The six sites of UL47 at Thr29, Ser30, Ser42, Thr47, Ser161, and Thr775 were identified as the phosphorylation targets of US3 protein. In vivo, UL47 phosphorylation was also detected but not in ΔUS3-infected cells. US3 protein promoted the cytoplasmic localization of UL47 at the late stage of infection, and the lack of US3 protein caused a delay in UL47 translocation to the cytoplasm. These results enhance our understanding of the functions of US3 during DPV infection and provide some references for DPV assembly. |
| first_indexed | 2024-04-11T07:21:51Z |
| format | Article |
| id | doaj.art-226b19bd29b741a9b0e950c28ee4d1ff |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T07:21:51Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-226b19bd29b741a9b0e950c28ee4d1ff2022-12-22T04:37:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.876820876820Duck plague virus US3 protein kinase phosphorylates UL47 and regulates the subcellular localization of UL47Liyao Deng0Liyao Deng1Liyao Deng2Jieyu Wan3Jieyu Wan4Jieyu Wan5Anchun Cheng6Anchun Cheng7Anchun Cheng8Mingshu Wang9Mingshu Wang10Mingshu Wang11Bin Tian12Bin Tian13Bin Tian14Ying Wu15Ying Wu16Ying Wu17Qiao Yang18Qiao Yang19Qiao Yang20Xumin Ou21Xumin Ou22Xumin Ou23Sai Mao24Sai Mao25Sai Mao26Di Sun27Di Sun28Di Sun29Shaqiu Zhang30Shaqiu Zhang31Shaqiu Zhang32Dekang Zhu33Dekang Zhu34Renyong Jia35Renyong Jia36Renyong Jia37Shun Chen38Shun Chen39Shun Chen40Mafeng Liu41Mafeng Liu42Mafeng Liu43Xinxin Zhao44Xinxin Zhao45Xinxin Zhao46Juan Huang47Juan Huang48Juan Huang49Qun Gao50Qun Gao51Qun Gao52Yanling Yu53Yanling Yu54Yanling Yu55Ling Zhang56Ling Zhang57Ling Zhang58Leichang Pan59Leichang Pan60Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaAvian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaDuck plague virus (DPV) belongs to the alphaherpesvirinae and causes high morbidity and mortality in waterfowl. UL47 is a large abundant structural protein in DPV, which means that UL47 protein plays an important role in virus replication. US3 protein, as a viral protein kinase in alphaherpesviruses, has been reported to be critical for DPV virion assembly. In this study, we over-expressed UL47 and US3 proteins and found that DPV UL47 protein was a phosphorylated substrate of US3 protein, which interacted and co-localized with US3 protein in the cytoplasm. US3-regulated phosphorylation of UL47 was important for the cytoplasmic localization of UL47 because non-phosphorylated UL47 was localized in the nucleus. The six sites of UL47 at Thr29, Ser30, Ser42, Thr47, Ser161, and Thr775 were identified as the phosphorylation targets of US3 protein. In vivo, UL47 phosphorylation was also detected but not in ΔUS3-infected cells. US3 protein promoted the cytoplasmic localization of UL47 at the late stage of infection, and the lack of US3 protein caused a delay in UL47 translocation to the cytoplasm. These results enhance our understanding of the functions of US3 during DPV infection and provide some references for DPV assembly.https://www.frontiersin.org/articles/10.3389/fmicb.2022.876820/fullduck plague virusUS3UL47phosphorylationsubcellular localization |
| spellingShingle | Liyao Deng Liyao Deng Liyao Deng Jieyu Wan Jieyu Wan Jieyu Wan Anchun Cheng Anchun Cheng Anchun Cheng Mingshu Wang Mingshu Wang Mingshu Wang Bin Tian Bin Tian Bin Tian Ying Wu Ying Wu Ying Wu Qiao Yang Qiao Yang Qiao Yang Xumin Ou Xumin Ou Xumin Ou Sai Mao Sai Mao Sai Mao Di Sun Di Sun Di Sun Shaqiu Zhang Shaqiu Zhang Shaqiu Zhang Dekang Zhu Dekang Zhu Renyong Jia Renyong Jia Renyong Jia Shun Chen Shun Chen Shun Chen Mafeng Liu Mafeng Liu Mafeng Liu Xinxin Zhao Xinxin Zhao Xinxin Zhao Juan Huang Juan Huang Juan Huang Qun Gao Qun Gao Qun Gao Yanling Yu Yanling Yu Yanling Yu Ling Zhang Ling Zhang Ling Zhang Leichang Pan Leichang Pan Duck plague virus US3 protein kinase phosphorylates UL47 and regulates the subcellular localization of UL47 Frontiers in Microbiology duck plague virus US3 UL47 phosphorylation subcellular localization |
| title | Duck plague virus US3 protein kinase phosphorylates UL47 and regulates the subcellular localization of UL47 |
| title_full | Duck plague virus US3 protein kinase phosphorylates UL47 and regulates the subcellular localization of UL47 |
| title_fullStr | Duck plague virus US3 protein kinase phosphorylates UL47 and regulates the subcellular localization of UL47 |
| title_full_unstemmed | Duck plague virus US3 protein kinase phosphorylates UL47 and regulates the subcellular localization of UL47 |
| title_short | Duck plague virus US3 protein kinase phosphorylates UL47 and regulates the subcellular localization of UL47 |
| title_sort | duck plague virus us3 protein kinase phosphorylates ul47 and regulates the subcellular localization of ul47 |
| topic | duck plague virus US3 UL47 phosphorylation subcellular localization |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.876820/full |
| work_keys_str_mv | AT liyaodeng duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT liyaodeng duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT liyaodeng duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT jieyuwan duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT jieyuwan duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT jieyuwan duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT anchuncheng duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT anchuncheng duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT anchuncheng duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT mingshuwang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT mingshuwang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT mingshuwang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT bintian duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT bintian duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT bintian duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT yingwu duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT yingwu duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT yingwu duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT qiaoyang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT qiaoyang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT qiaoyang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT xuminou duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT xuminou duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT xuminou duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT saimao duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT saimao duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT saimao duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT disun duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT disun duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT disun duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT shaqiuzhang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT shaqiuzhang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT shaqiuzhang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT dekangzhu duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT dekangzhu duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT renyongjia duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT renyongjia duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT renyongjia duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT shunchen duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT shunchen duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT shunchen duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT mafengliu duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT mafengliu duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT mafengliu duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT xinxinzhao duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT xinxinzhao duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT xinxinzhao duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT juanhuang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT juanhuang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT juanhuang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT qungao duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT qungao duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT qungao duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT yanlingyu duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT yanlingyu duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT yanlingyu duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT lingzhang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT lingzhang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT lingzhang duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT leichangpan duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 AT leichangpan duckplaguevirusus3proteinkinasephosphorylatesul47andregulatesthesubcellularlocalizationoful47 |