Improving the expression of recombinant pullulanase by increasing mRNA stability in Escherichia coli

Improving the expression of recombinant pullulanase by increasing mRNA stability in Escherichia coli

Background: Pullulanase production in both wild-type strains and recombinantly engineered strains remains low. The Shine-Dalgarno (SD) sequence and stem-loop structure in the 5′ or 3′ untranslated region (UTR) are well-known determinants of mRNA stability. This study investigated the effect of mRNA...

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Bibliographic Details
Main Authors: Tao Li, Yatong Ding, Jun Zhang, Guobao Jiao, Lipeng Sun, Zhongmin Liu, Liyou Qiu
Format: Article
Language:English
Published: Elsevier 2017-09-01
Series:Electronic Journal of Biotechnology
Subjects:
Amylases
Carbohydrate debranching enzyme
Enzyme activity
Expression vector
Gene expression
Starch hydrolysis
Industrial applications
Pullulanase fermentation
SD sequence
Semi-quantitative RT-PCR
Stem-loop structure
Online Access:http://www.sciencedirect.com/science/article/pii/S0717345817300416

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