Negatively Charged Composite Nanofibrous Hydrogel Membranes for High-Performance Protein Adsorption
Nanofibrous materials are considered as promising candidates for fabricating high-efficiency chromatography media, which are urgently needed in protein pharmaceuticals purification and biological research, yet still face several bottlenecks. Herein, novel negatively charged composite nanofibrous hyd...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/19/3500 |
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| author | Qiuxia Fu Dandan Xie Jianlong Ge Wei Zhang Haoru Shan |
| author_facet | Qiuxia Fu Dandan Xie Jianlong Ge Wei Zhang Haoru Shan |
| author_sort | Qiuxia Fu |
| collection | DOAJ |
| description | Nanofibrous materials are considered as promising candidates for fabricating high-efficiency chromatography media, which are urgently needed in protein pharmaceuticals purification and biological research, yet still face several bottlenecks. Herein, novel negatively charged composite nanofibrous hydrogel membranes (NHMs) are obtained by a facile combination of electrospinning and surface coating modification. The resulting NHMs exhibit controllable morphologies and chemical structures. Benefitting from the combined effect of the stable framework of silicon dioxide (SiO<sub>2</sub>) nanofiber and the function layer of negatively charged hydrogel, as well as good pore connectivity among nanofibers, NHMs exhibit a high protein adsorption capacity of around 1000 mg g<sup>−1</sup>, and are superior to the commercial cellulose fibrous adsorbent (Sartobind<sup>®</sup>) and the reported nanofibrous membranous adsorbents. Moreover, due to their relatively stable physicochemical and mechanical properties, NHMs possess comprehensive adsorption performance, favorable resistance to acid and solvents, good selectivity, and excellent regenerability. The designed NHMs composite adsorbents are expected to supply a new protein chromatography platform for effective protein purification in biopharmaceuticals and biochemical reagents. |
| first_indexed | 2024-03-09T21:20:33Z |
| format | Article |
| id | doaj.art-2277a641f9a94cff9eea070dc1586180 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T21:20:33Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
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| series | Nanomaterials |
| spelling | doaj.art-2277a641f9a94cff9eea070dc15861802023-11-23T21:21:06ZengMDPI AGNanomaterials2079-49912022-10-011219350010.3390/nano12193500Negatively Charged Composite Nanofibrous Hydrogel Membranes for High-Performance Protein AdsorptionQiuxia Fu0Dandan Xie1Jianlong Ge2Wei Zhang3Haoru Shan4School of Textile and Clothing, Nantong University, Nantong 226019, ChinaSchool of Textile and Clothing, Nantong University, Nantong 226019, ChinaSchool of Textile and Clothing, Nantong University, Nantong 226019, ChinaSchool of Textile and Clothing, Nantong University, Nantong 226019, ChinaSchool of Textile and Clothing, Nantong University, Nantong 226019, ChinaNanofibrous materials are considered as promising candidates for fabricating high-efficiency chromatography media, which are urgently needed in protein pharmaceuticals purification and biological research, yet still face several bottlenecks. Herein, novel negatively charged composite nanofibrous hydrogel membranes (NHMs) are obtained by a facile combination of electrospinning and surface coating modification. The resulting NHMs exhibit controllable morphologies and chemical structures. Benefitting from the combined effect of the stable framework of silicon dioxide (SiO<sub>2</sub>) nanofiber and the function layer of negatively charged hydrogel, as well as good pore connectivity among nanofibers, NHMs exhibit a high protein adsorption capacity of around 1000 mg g<sup>−1</sup>, and are superior to the commercial cellulose fibrous adsorbent (Sartobind<sup>®</sup>) and the reported nanofibrous membranous adsorbents. Moreover, due to their relatively stable physicochemical and mechanical properties, NHMs possess comprehensive adsorption performance, favorable resistance to acid and solvents, good selectivity, and excellent regenerability. The designed NHMs composite adsorbents are expected to supply a new protein chromatography platform for effective protein purification in biopharmaceuticals and biochemical reagents.https://www.mdpi.com/2079-4991/12/19/3500electrospun nanofibersfunctional hydrogel layerscomposite membraneselectronegativityprotein adsorption |
| spellingShingle | Qiuxia Fu Dandan Xie Jianlong Ge Wei Zhang Haoru Shan Negatively Charged Composite Nanofibrous Hydrogel Membranes for High-Performance Protein Adsorption Nanomaterials electrospun nanofibers functional hydrogel layers composite membranes electronegativity protein adsorption |
| title | Negatively Charged Composite Nanofibrous Hydrogel Membranes for High-Performance Protein Adsorption |
| title_full | Negatively Charged Composite Nanofibrous Hydrogel Membranes for High-Performance Protein Adsorption |
| title_fullStr | Negatively Charged Composite Nanofibrous Hydrogel Membranes for High-Performance Protein Adsorption |
| title_full_unstemmed | Negatively Charged Composite Nanofibrous Hydrogel Membranes for High-Performance Protein Adsorption |
| title_short | Negatively Charged Composite Nanofibrous Hydrogel Membranes for High-Performance Protein Adsorption |
| title_sort | negatively charged composite nanofibrous hydrogel membranes for high performance protein adsorption |
| topic | electrospun nanofibers functional hydrogel layers composite membranes electronegativity protein adsorption |
| url | https://www.mdpi.com/2079-4991/12/19/3500 |
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