Effect of Different Physical Factors on efficacy of Thevetia Peruviana leaf extract and bio-formulations
Plant extract possess various secondary metabolites which are antifungal in nature and can be used as a safer alternative to the synthetic fungicides. As we all know that the chemical fungicides are harmful not only for humans but also for animals, other vegetation and for complete ecosystem. To ove...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580822000711 |
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| author | Bhanu Raj Meena Deepali Chittora Jyoti Yadav Sanjeev Meena Suresh Kumar Tripta Jain Kanika Sharma |
| author_facet | Bhanu Raj Meena Deepali Chittora Jyoti Yadav Sanjeev Meena Suresh Kumar Tripta Jain Kanika Sharma |
| author_sort | Bhanu Raj Meena |
| collection | DOAJ |
| description | Plant extract possess various secondary metabolites which are antifungal in nature and can be used as a safer alternative to the synthetic fungicides. As we all know that the chemical fungicides are harmful not only for humans but also for animals, other vegetation and for complete ecosystem. To overcome this problem, we have to focused on another alternative which are biologically libel and nonhazardous also. In the present study, herbal formulation was prepared in various combination ratios with Thevetia peruviana leaf extracts, cow dung and neem oil cake. The major aim of this short study is to check the stability of the said plant extracts and prepared herbal formulation on various physical factors like heat, temperature, pH, sunlight and storage etc. The extracts and herbal formulations were exposed to varying conditions of the parameters selected for a precise time period, and then observing the effect as a function of change in the crude extract activity, herbal formulation activity and change minimum inhibitory concentration of plant extract against the Alternaria solani. Control set of MIC, and extract free medium were maintained for comparison in each set of experiment against Alternaria solani. Results suggested that efficacy of leaf extracts and different formulations was not affected by wet heat up to 100 °C while slight reduction in antifungal activity of the plant extract and herbal formulations were observed with dry heat at 100 °C. In addition, slight reduction in activity of extract and herbal formulations was observed with change in pH. However antifungal activity of plant extract as well as herbal formulations, remain unaffected at alkaline pH (pH 9) and neutral pH (pH7). Storage for 6 and 12 months had no negative effect on extract and herbal formulation efficacy and the antifungal activity was observed similar to freshly prepared extract activity. The present study concluded that the plant disease or plant pathogens can be controlled by plant extract and plant based bioformulations by increasing the shelf life with some little changes in the physical parameters such as light, temperature, pH and storage. |
| first_indexed | 2024-12-12T09:39:17Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-12T09:39:17Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-2278f0a9bd20460cb5bdbf0383df57b12022-12-22T00:28:37ZengElsevierBiochemistry and Biophysics Reports2405-58082022-07-0130101271Effect of Different Physical Factors on efficacy of Thevetia Peruviana leaf extract and bio-formulationsBhanu Raj Meena0Deepali Chittora1Jyoti Yadav2Sanjeev Meena3Suresh Kumar4Tripta Jain5Kanika Sharma6Microbial Research Laboratory, Department of Botany, University College of Science, Mohanlal Sukhadia University, Udaipur, Rajasthan, India; Corresponding author.Microbial Research Laboratory, Department of Botany, University College of Science, Mohanlal Sukhadia University, Udaipur, Rajasthan, IndiaMicrobial Research Laboratory, Department of Botany, University College of Science, Mohanlal Sukhadia University, Udaipur, Rajasthan, IndiaMicrobial Research Laboratory, Department of Botany, University College of Science, Mohanlal Sukhadia University, Udaipur, Rajasthan, IndiaDepartment of Botany, Ramjas college, University of Delhi, IndiaMicrobial Research Laboratory, Department of Botany, University College of Science, Mohanlal Sukhadia University, Udaipur, Rajasthan, IndiaMicrobial Research Laboratory, Department of Botany, University College of Science, Mohanlal Sukhadia University, Udaipur, Rajasthan, IndiaPlant extract possess various secondary metabolites which are antifungal in nature and can be used as a safer alternative to the synthetic fungicides. As we all know that the chemical fungicides are harmful not only for humans but also for animals, other vegetation and for complete ecosystem. To overcome this problem, we have to focused on another alternative which are biologically libel and nonhazardous also. In the present study, herbal formulation was prepared in various combination ratios with Thevetia peruviana leaf extracts, cow dung and neem oil cake. The major aim of this short study is to check the stability of the said plant extracts and prepared herbal formulation on various physical factors like heat, temperature, pH, sunlight and storage etc. The extracts and herbal formulations were exposed to varying conditions of the parameters selected for a precise time period, and then observing the effect as a function of change in the crude extract activity, herbal formulation activity and change minimum inhibitory concentration of plant extract against the Alternaria solani. Control set of MIC, and extract free medium were maintained for comparison in each set of experiment against Alternaria solani. Results suggested that efficacy of leaf extracts and different formulations was not affected by wet heat up to 100 °C while slight reduction in antifungal activity of the plant extract and herbal formulations were observed with dry heat at 100 °C. In addition, slight reduction in activity of extract and herbal formulations was observed with change in pH. However antifungal activity of plant extract as well as herbal formulations, remain unaffected at alkaline pH (pH 9) and neutral pH (pH7). Storage for 6 and 12 months had no negative effect on extract and herbal formulation efficacy and the antifungal activity was observed similar to freshly prepared extract activity. The present study concluded that the plant disease or plant pathogens can be controlled by plant extract and plant based bioformulations by increasing the shelf life with some little changes in the physical parameters such as light, temperature, pH and storage.http://www.sciencedirect.com/science/article/pii/S2405580822000711Secondary metabolitesAntifungal agentsSynthetic fungicidesHerbal formulationThevetia |
| spellingShingle | Bhanu Raj Meena Deepali Chittora Jyoti Yadav Sanjeev Meena Suresh Kumar Tripta Jain Kanika Sharma Effect of Different Physical Factors on efficacy of Thevetia Peruviana leaf extract and bio-formulations Biochemistry and Biophysics Reports Secondary metabolites Antifungal agents Synthetic fungicides Herbal formulation Thevetia |
| title | Effect of Different Physical Factors on efficacy of Thevetia Peruviana leaf extract and bio-formulations |
| title_full | Effect of Different Physical Factors on efficacy of Thevetia Peruviana leaf extract and bio-formulations |
| title_fullStr | Effect of Different Physical Factors on efficacy of Thevetia Peruviana leaf extract and bio-formulations |
| title_full_unstemmed | Effect of Different Physical Factors on efficacy of Thevetia Peruviana leaf extract and bio-formulations |
| title_short | Effect of Different Physical Factors on efficacy of Thevetia Peruviana leaf extract and bio-formulations |
| title_sort | effect of different physical factors on efficacy of thevetia peruviana leaf extract and bio formulations |
| topic | Secondary metabolites Antifungal agents Synthetic fungicides Herbal formulation Thevetia |
| url | http://www.sciencedirect.com/science/article/pii/S2405580822000711 |
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