Bacterial and Fungal Profile, Antibiotic Susceptibility Patterns of Bacterial Pathogens and Associated Risk Factors of Urinary Tract Infection Among Symptomatic Pediatrics Patients Attending St. Paul’s Hospital Millennium Medical College: A Cross-Sectional Study
Adane Bitew,1 Nuhamen Zena,2 Abera Abdeta3 1Department of Medical Laboratory Science, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 2Department of Microbiology, St Paul Hospital Millennium Medical College, Addis Ababa, Ethiopia; 3National Clinical Bacteriology and Mycolo...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-04-01
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| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/bacterial-and-fungal-profile-antibiotic-susceptibility-patterns-of-bac-peer-reviewed-fulltext-article-IDR |
| _version_ | 1828291268173627392 |
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| author | Bitew A Zena N Abdeta A |
| author_facet | Bitew A Zena N Abdeta A |
| author_sort | Bitew A |
| collection | DOAJ |
| description | Adane Bitew,1 Nuhamen Zena,2 Abera Abdeta3 1Department of Medical Laboratory Science, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 2Department of Microbiology, St Paul Hospital Millennium Medical College, Addis Ababa, Ethiopia; 3National Clinical Bacteriology and Mycology Reference Laboratory, Ethiopian Public Health Institute, Addis Ababa, EthiopiaCorrespondence: Abera Abdeta, National Clinical Bacteriology and Mycology Reference Laboratory, Ethiopian public health institute, P.O. Box:1242, Addis Ababa, Ethiopia, Tel +251911566420, Email aberaabdeta4@gmail.comBackground: Urinary tract infection is a common infection in pediatrics. Understanding the common etiology of urinary tract infections, their antimicrobial susceptibility pattern, and associated risk factors in a particular setting can provide evidence for the appropriate treatment of the cases.Purpose: The current study is aimed to determine the common etiology and prevalence of uropathogens associated with urinary tract infection, as well as the antibiotic susceptibility profile of bacterial isolates, and to identify risk factors associated with urinary tract infection among pediatric patients.Materials and Methods: The study was conducted at St. Paul Hospital Millennium Medical College between October 2019 and July 2020. Urine was collected aseptically from patients, inoculated onto culture media, and incubated at 37 °C for 18– 48 hours. Bacteria and yeast were identified following standard procedures. Antibiotic susceptibility testing of bacterial pathogens was carried out by the Kirby Bauer disc diffusion method. Descriptive statistics and logistical regressions were used to estimate the crude ratio with a 95% confidence interval. P-value < 0.05 was considered significant.Results: Significant bacterial/fungal growth was observed in 65 samples giving a prevalence of 28.6% of which 75.4% (49/65) and 24.6% (16/65) were bacterial and fungal pathogens, respectively. About 79.6% of bacterial etiology were Escherichia coli and Klebsiella pneumoniae. The highest resistance was observed against ampicillin (100%), cefazolin (92.1%), and trimethoprim-sulfamethoxazole (84.1%), both of which are commonly used for empirical treatment in Ethiopia. Length of hospital stay (P=0.01) and catheterization (P=0.04) were statistically associated with urinary tract infection.Conclusion: The high prevalence of urinary tract infection was observed in our study. Enterobacteriaceae were the major cause of urinary tract infection. Length of hospital stay and catheterization were significantly associated with urinary tract infection. Both Gram-negative and Gram-positive bacteria were extremely resistant to ampicillin and trimethoprim-sulfamethoxazole.Keywords: antibiotic susceptibility pattern, pediatrics, urinary tract infection, Ethiopia |
| first_indexed | 2024-04-13T10:49:49Z |
| format | Article |
| id | doaj.art-2279fd7d049d475b92e40468ff4e644a |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-04-13T10:49:49Z |
| publishDate | 2022-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-2279fd7d049d475b92e40468ff4e644a2022-12-22T02:49:41ZengDove Medical PressInfection and Drug Resistance1178-69732022-04-01Volume 151613162474280Bacterial and Fungal Profile, Antibiotic Susceptibility Patterns of Bacterial Pathogens and Associated Risk Factors of Urinary Tract Infection Among Symptomatic Pediatrics Patients Attending St. Paul’s Hospital Millennium Medical College: A Cross-Sectional StudyBitew AZena NAbdeta AAdane Bitew,1 Nuhamen Zena,2 Abera Abdeta3 1Department of Medical Laboratory Science, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 2Department of Microbiology, St Paul Hospital Millennium Medical College, Addis Ababa, Ethiopia; 3National Clinical Bacteriology and Mycology Reference Laboratory, Ethiopian Public Health Institute, Addis Ababa, EthiopiaCorrespondence: Abera Abdeta, National Clinical Bacteriology and Mycology Reference Laboratory, Ethiopian public health institute, P.O. Box:1242, Addis Ababa, Ethiopia, Tel +251911566420, Email aberaabdeta4@gmail.comBackground: Urinary tract infection is a common infection in pediatrics. Understanding the common etiology of urinary tract infections, their antimicrobial susceptibility pattern, and associated risk factors in a particular setting can provide evidence for the appropriate treatment of the cases.Purpose: The current study is aimed to determine the common etiology and prevalence of uropathogens associated with urinary tract infection, as well as the antibiotic susceptibility profile of bacterial isolates, and to identify risk factors associated with urinary tract infection among pediatric patients.Materials and Methods: The study was conducted at St. Paul Hospital Millennium Medical College between October 2019 and July 2020. Urine was collected aseptically from patients, inoculated onto culture media, and incubated at 37 °C for 18– 48 hours. Bacteria and yeast were identified following standard procedures. Antibiotic susceptibility testing of bacterial pathogens was carried out by the Kirby Bauer disc diffusion method. Descriptive statistics and logistical regressions were used to estimate the crude ratio with a 95% confidence interval. P-value < 0.05 was considered significant.Results: Significant bacterial/fungal growth was observed in 65 samples giving a prevalence of 28.6% of which 75.4% (49/65) and 24.6% (16/65) were bacterial and fungal pathogens, respectively. About 79.6% of bacterial etiology were Escherichia coli and Klebsiella pneumoniae. The highest resistance was observed against ampicillin (100%), cefazolin (92.1%), and trimethoprim-sulfamethoxazole (84.1%), both of which are commonly used for empirical treatment in Ethiopia. Length of hospital stay (P=0.01) and catheterization (P=0.04) were statistically associated with urinary tract infection.Conclusion: The high prevalence of urinary tract infection was observed in our study. Enterobacteriaceae were the major cause of urinary tract infection. Length of hospital stay and catheterization were significantly associated with urinary tract infection. Both Gram-negative and Gram-positive bacteria were extremely resistant to ampicillin and trimethoprim-sulfamethoxazole.Keywords: antibiotic susceptibility pattern, pediatrics, urinary tract infection, Ethiopiahttps://www.dovepress.com/bacterial-and-fungal-profile-antibiotic-susceptibility-patterns-of-bac-peer-reviewed-fulltext-article-IDRantibiotic susceptibility patternpediatricsurinary tract infectionethiopia |
| spellingShingle | Bitew A Zena N Abdeta A Bacterial and Fungal Profile, Antibiotic Susceptibility Patterns of Bacterial Pathogens and Associated Risk Factors of Urinary Tract Infection Among Symptomatic Pediatrics Patients Attending St. Paul’s Hospital Millennium Medical College: A Cross-Sectional Study Infection and Drug Resistance antibiotic susceptibility pattern pediatrics urinary tract infection ethiopia |
| title | Bacterial and Fungal Profile, Antibiotic Susceptibility Patterns of Bacterial Pathogens and Associated Risk Factors of Urinary Tract Infection Among Symptomatic Pediatrics Patients Attending St. Paul’s Hospital Millennium Medical College: A Cross-Sectional Study |
| title_full | Bacterial and Fungal Profile, Antibiotic Susceptibility Patterns of Bacterial Pathogens and Associated Risk Factors of Urinary Tract Infection Among Symptomatic Pediatrics Patients Attending St. Paul’s Hospital Millennium Medical College: A Cross-Sectional Study |
| title_fullStr | Bacterial and Fungal Profile, Antibiotic Susceptibility Patterns of Bacterial Pathogens and Associated Risk Factors of Urinary Tract Infection Among Symptomatic Pediatrics Patients Attending St. Paul’s Hospital Millennium Medical College: A Cross-Sectional Study |
| title_full_unstemmed | Bacterial and Fungal Profile, Antibiotic Susceptibility Patterns of Bacterial Pathogens and Associated Risk Factors of Urinary Tract Infection Among Symptomatic Pediatrics Patients Attending St. Paul’s Hospital Millennium Medical College: A Cross-Sectional Study |
| title_short | Bacterial and Fungal Profile, Antibiotic Susceptibility Patterns of Bacterial Pathogens and Associated Risk Factors of Urinary Tract Infection Among Symptomatic Pediatrics Patients Attending St. Paul’s Hospital Millennium Medical College: A Cross-Sectional Study |
| title_sort | bacterial and fungal profile antibiotic susceptibility patterns of bacterial pathogens and associated risk factors of urinary tract infection among symptomatic pediatrics patients attending st paul rsquo s hospital millennium medical college a cross sectional study |
| topic | antibiotic susceptibility pattern pediatrics urinary tract infection ethiopia |
| url | https://www.dovepress.com/bacterial-and-fungal-profile-antibiotic-susceptibility-patterns-of-bac-peer-reviewed-fulltext-article-IDR |
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