Fractionation of Extracts from Black Chokeberry, Cranberry, and Pomegranate to Identify Compounds That Influence Lipid Metabolism
Polyphenols show a spectrum of bioactive effects, including an influence on lipid metabolism. In this study, we performed activity-guided fractionations of black chokeberry (aronia), cranberry, and pomegranate extracts to identify the biologically active compounds. The extracts were prepared from fr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/4/570 |
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| author | Sonja Niesen Celina Göttel Hanna Becker Tamara Bakuradze Peter Winterhalter Elke Richling |
| author_facet | Sonja Niesen Celina Göttel Hanna Becker Tamara Bakuradze Peter Winterhalter Elke Richling |
| author_sort | Sonja Niesen |
| collection | DOAJ |
| description | Polyphenols show a spectrum of bioactive effects, including an influence on lipid metabolism. In this study, we performed activity-guided fractionations of black chokeberry (aronia), cranberry, and pomegranate extracts to identify the biologically active compounds. The extracts were prepared from fruit juice concentrates with the adsorbent resin Amberlite XAD-7 and were separated into a copigment and an anthocyanin fraction, followed by fractionation into a polymer and monomeric fraction by means of hexane precipitation. For further fractionation of the cranberry and pomegranate copigment fractions, high-performance countercurrent chromatography (HPCCC) was used. The compounds in each fraction were identified by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS), and the quantification was performed by ultra high-performance liquid chromatography-diode array detector (UHPLC-DAD) analyses. Each of the (sub-)fractions was tested in three in vitro assays: phosphodiesterase 3B (PDE) activity, lipid accumulation, and lipolysis in 3T3-L1 cells. The results showed that various fractions and subfractions can inhibit lipid accumulation and PDE activity as well as increase lipolysis, particularly copigments. Overall, our results indicate an influence of polyphenol-rich (sub-)fractions on the lipid metabolism. |
| first_indexed | 2024-03-09T21:58:22Z |
| format | Article |
| id | doaj.art-227aa1d7246d4ca6b84ab6be3c909d66 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T21:58:22Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-227aa1d7246d4ca6b84ab6be3c909d662023-11-23T19:53:56ZengMDPI AGFoods2304-81582022-02-0111457010.3390/foods11040570Fractionation of Extracts from Black Chokeberry, Cranberry, and Pomegranate to Identify Compounds That Influence Lipid MetabolismSonja Niesen0Celina Göttel1Hanna Becker2Tamara Bakuradze3Peter Winterhalter4Elke Richling5Institute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstraße 20, D-38106 Braunschweig, GermanyDivision of Food Chemistry and Toxicology, Department of Chemistry, Technische Universität Kaiserslautern, Erwin-Schrödinger-Straße 52, D-67663 Kaiserslautern, GermanyDivision of Food Chemistry and Toxicology, Department of Chemistry, Technische Universität Kaiserslautern, Erwin-Schrödinger-Straße 52, D-67663 Kaiserslautern, GermanyDivision of Food Chemistry and Toxicology, Department of Chemistry, Technische Universität Kaiserslautern, Erwin-Schrödinger-Straße 52, D-67663 Kaiserslautern, GermanyInstitute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstraße 20, D-38106 Braunschweig, GermanyDivision of Food Chemistry and Toxicology, Department of Chemistry, Technische Universität Kaiserslautern, Erwin-Schrödinger-Straße 52, D-67663 Kaiserslautern, GermanyPolyphenols show a spectrum of bioactive effects, including an influence on lipid metabolism. In this study, we performed activity-guided fractionations of black chokeberry (aronia), cranberry, and pomegranate extracts to identify the biologically active compounds. The extracts were prepared from fruit juice concentrates with the adsorbent resin Amberlite XAD-7 and were separated into a copigment and an anthocyanin fraction, followed by fractionation into a polymer and monomeric fraction by means of hexane precipitation. For further fractionation of the cranberry and pomegranate copigment fractions, high-performance countercurrent chromatography (HPCCC) was used. The compounds in each fraction were identified by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS), and the quantification was performed by ultra high-performance liquid chromatography-diode array detector (UHPLC-DAD) analyses. Each of the (sub-)fractions was tested in three in vitro assays: phosphodiesterase 3B (PDE) activity, lipid accumulation, and lipolysis in 3T3-L1 cells. The results showed that various fractions and subfractions can inhibit lipid accumulation and PDE activity as well as increase lipolysis, particularly copigments. Overall, our results indicate an influence of polyphenol-rich (sub-)fractions on the lipid metabolism.https://www.mdpi.com/2304-8158/11/4/570lipid accumulationlipolysisphosphodiesterase activityanthocyaninscopigmentspolymers |
| spellingShingle | Sonja Niesen Celina Göttel Hanna Becker Tamara Bakuradze Peter Winterhalter Elke Richling Fractionation of Extracts from Black Chokeberry, Cranberry, and Pomegranate to Identify Compounds That Influence Lipid Metabolism Foods lipid accumulation lipolysis phosphodiesterase activity anthocyanins copigments polymers |
| title | Fractionation of Extracts from Black Chokeberry, Cranberry, and Pomegranate to Identify Compounds That Influence Lipid Metabolism |
| title_full | Fractionation of Extracts from Black Chokeberry, Cranberry, and Pomegranate to Identify Compounds That Influence Lipid Metabolism |
| title_fullStr | Fractionation of Extracts from Black Chokeberry, Cranberry, and Pomegranate to Identify Compounds That Influence Lipid Metabolism |
| title_full_unstemmed | Fractionation of Extracts from Black Chokeberry, Cranberry, and Pomegranate to Identify Compounds That Influence Lipid Metabolism |
| title_short | Fractionation of Extracts from Black Chokeberry, Cranberry, and Pomegranate to Identify Compounds That Influence Lipid Metabolism |
| title_sort | fractionation of extracts from black chokeberry cranberry and pomegranate to identify compounds that influence lipid metabolism |
| topic | lipid accumulation lipolysis phosphodiesterase activity anthocyanins copigments polymers |
| url | https://www.mdpi.com/2304-8158/11/4/570 |
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