<i>Acinetobacter baumannii</i> IC2 and IC5 Isolates with Co-Existing <i>bla</i><sub>OXA-143-like</sub> and <i>bla</i><sub>OXA-72</sub> and Exhibiting Strong Biofilm Formation in a Mexican Hospital
<i>Acinetobacter baumannii</i> is an opportunistic pathogen responsible for healthcare-associated infections (HAIs) and outbreaks. Antimicrobial resistance mechanisms and virulence factors allow it to survive and spread in the hospital environment. However, the molecular mechanisms of th...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/11/9/2316 |
| _version_ | 1827725181885349888 |
|---|---|
| author | Julia Moreno-Manjón Santiago Castillo-Ramírez Keith A. Jolley Martin C. J. Maiden Catalina Gayosso-Vázquez José Luis Fernández-Vázquez Valeria Mateo-Estrada Silvia Giono-Cerezo María Dolores Alcántar-Curiel |
| author_facet | Julia Moreno-Manjón Santiago Castillo-Ramírez Keith A. Jolley Martin C. J. Maiden Catalina Gayosso-Vázquez José Luis Fernández-Vázquez Valeria Mateo-Estrada Silvia Giono-Cerezo María Dolores Alcántar-Curiel |
| author_sort | Julia Moreno-Manjón |
| collection | DOAJ |
| description | <i>Acinetobacter baumannii</i> is an opportunistic pathogen responsible for healthcare-associated infections (HAIs) and outbreaks. Antimicrobial resistance mechanisms and virulence factors allow it to survive and spread in the hospital environment. However, the molecular mechanisms of these traits and their association with international clones are frequently unknown in low- and middle-income countries. Here, we analyze the phenotype and genotype of seventy-six HAIs and outbreak-causing <i>A. baumannii</i> isolates from a Mexican hospital over ten years, with special attention to the carbapenem resistome and biofilm formation. The isolates belonged to the global international clone (IC) 2 and the Latin America endemic IC5 and were predominantly extensively drug-resistant (XDR). Oxacillinases were identified as a common source of carbapenem resistance. We noted the presence of the <i>bla</i><sub>OXA-143-like</sub> family (not previously described in Mexico), the <i>bla</i><sub>OXA-72</sub> and the <i>bla</i><sub>OXA-398</sub> found in both ICs. A low prevalence of efflux pump overexpression activity associated with carbapenem resistance was observed. Finally, strong biofilm formation was found, and significant biofilm-related genes were identified, including <i>bfmRS</i>, <i>csuA/BABCDE</i>, <i>pgaABCD</i> and <i>ompA.</i> This study provides a comprehensive profile of the carbapenem resistome of <i>A. baumannii</i> isolates belonging to the same pulse type, along with their significant biofilm formation capacity. Furthermore, it contributes to a better understanding of their role in the recurrence of infection and the endemicity of these isolates in a Mexican hospital. |
| first_indexed | 2024-03-10T22:25:49Z |
| format | Article |
| id | doaj.art-227b49f9557847d0a121202a8879b8e2 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T22:25:49Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-227b49f9557847d0a121202a8879b8e22023-11-19T12:03:38ZengMDPI AGMicroorganisms2076-26072023-09-01119231610.3390/microorganisms11092316<i>Acinetobacter baumannii</i> IC2 and IC5 Isolates with Co-Existing <i>bla</i><sub>OXA-143-like</sub> and <i>bla</i><sub>OXA-72</sub> and Exhibiting Strong Biofilm Formation in a Mexican HospitalJulia Moreno-Manjón0Santiago Castillo-Ramírez1Keith A. Jolley2Martin C. J. Maiden3Catalina Gayosso-Vázquez4José Luis Fernández-Vázquez5Valeria Mateo-Estrada6Silvia Giono-Cerezo7María Dolores Alcántar-Curiel8Laboratorio de Infectología, Microbiología e Inmunología Clínica, Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México 06720, MexicoPrograma de Genómica Evolutiva, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca 62209, MexicoDepartment of Biology, University of Oxford, Oxford OX1 3SZ, UKDepartment of Biology, University of Oxford, Oxford OX1 3SZ, UKLaboratorio de Infectología, Microbiología e Inmunología Clínica, Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México 06720, MexicoLaboratorio de Infectología, Microbiología e Inmunología Clínica, Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México 06720, MexicoPrograma de Genómica Evolutiva, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca 62209, MexicoLaboratorio de Bacteriología Médica, Posgrado en Ciencias Quimicobiológicas, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México 11350, MexicoLaboratorio de Infectología, Microbiología e Inmunología Clínica, Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México 06720, Mexico<i>Acinetobacter baumannii</i> is an opportunistic pathogen responsible for healthcare-associated infections (HAIs) and outbreaks. Antimicrobial resistance mechanisms and virulence factors allow it to survive and spread in the hospital environment. However, the molecular mechanisms of these traits and their association with international clones are frequently unknown in low- and middle-income countries. Here, we analyze the phenotype and genotype of seventy-six HAIs and outbreak-causing <i>A. baumannii</i> isolates from a Mexican hospital over ten years, with special attention to the carbapenem resistome and biofilm formation. The isolates belonged to the global international clone (IC) 2 and the Latin America endemic IC5 and were predominantly extensively drug-resistant (XDR). Oxacillinases were identified as a common source of carbapenem resistance. We noted the presence of the <i>bla</i><sub>OXA-143-like</sub> family (not previously described in Mexico), the <i>bla</i><sub>OXA-72</sub> and the <i>bla</i><sub>OXA-398</sub> found in both ICs. A low prevalence of efflux pump overexpression activity associated with carbapenem resistance was observed. Finally, strong biofilm formation was found, and significant biofilm-related genes were identified, including <i>bfmRS</i>, <i>csuA/BABCDE</i>, <i>pgaABCD</i> and <i>ompA.</i> This study provides a comprehensive profile of the carbapenem resistome of <i>A. baumannii</i> isolates belonging to the same pulse type, along with their significant biofilm formation capacity. Furthermore, it contributes to a better understanding of their role in the recurrence of infection and the endemicity of these isolates in a Mexican hospital.https://www.mdpi.com/2076-2607/11/9/2316resistomebiofilm formationantimicrobial resistancehealthcare-associated infectionsoutbreakgenomic epidemiology |
| spellingShingle | Julia Moreno-Manjón Santiago Castillo-Ramírez Keith A. Jolley Martin C. J. Maiden Catalina Gayosso-Vázquez José Luis Fernández-Vázquez Valeria Mateo-Estrada Silvia Giono-Cerezo María Dolores Alcántar-Curiel <i>Acinetobacter baumannii</i> IC2 and IC5 Isolates with Co-Existing <i>bla</i><sub>OXA-143-like</sub> and <i>bla</i><sub>OXA-72</sub> and Exhibiting Strong Biofilm Formation in a Mexican Hospital Microorganisms resistome biofilm formation antimicrobial resistance healthcare-associated infections outbreak genomic epidemiology |
| title | <i>Acinetobacter baumannii</i> IC2 and IC5 Isolates with Co-Existing <i>bla</i><sub>OXA-143-like</sub> and <i>bla</i><sub>OXA-72</sub> and Exhibiting Strong Biofilm Formation in a Mexican Hospital |
| title_full | <i>Acinetobacter baumannii</i> IC2 and IC5 Isolates with Co-Existing <i>bla</i><sub>OXA-143-like</sub> and <i>bla</i><sub>OXA-72</sub> and Exhibiting Strong Biofilm Formation in a Mexican Hospital |
| title_fullStr | <i>Acinetobacter baumannii</i> IC2 and IC5 Isolates with Co-Existing <i>bla</i><sub>OXA-143-like</sub> and <i>bla</i><sub>OXA-72</sub> and Exhibiting Strong Biofilm Formation in a Mexican Hospital |
| title_full_unstemmed | <i>Acinetobacter baumannii</i> IC2 and IC5 Isolates with Co-Existing <i>bla</i><sub>OXA-143-like</sub> and <i>bla</i><sub>OXA-72</sub> and Exhibiting Strong Biofilm Formation in a Mexican Hospital |
| title_short | <i>Acinetobacter baumannii</i> IC2 and IC5 Isolates with Co-Existing <i>bla</i><sub>OXA-143-like</sub> and <i>bla</i><sub>OXA-72</sub> and Exhibiting Strong Biofilm Formation in a Mexican Hospital |
| title_sort | i acinetobacter baumannii i ic2 and ic5 isolates with co existing i bla i sub oxa 143 like sub and i bla i sub oxa 72 sub and exhibiting strong biofilm formation in a mexican hospital |
| topic | resistome biofilm formation antimicrobial resistance healthcare-associated infections outbreak genomic epidemiology |
| url | https://www.mdpi.com/2076-2607/11/9/2316 |
| work_keys_str_mv | AT juliamorenomanjon iacinetobacterbaumanniiiic2andic5isolateswithcoexistingiblaisuboxa143likesubandiblaisuboxa72subandexhibitingstrongbiofilmformationinamexicanhospital AT santiagocastilloramirez iacinetobacterbaumanniiiic2andic5isolateswithcoexistingiblaisuboxa143likesubandiblaisuboxa72subandexhibitingstrongbiofilmformationinamexicanhospital AT keithajolley iacinetobacterbaumanniiiic2andic5isolateswithcoexistingiblaisuboxa143likesubandiblaisuboxa72subandexhibitingstrongbiofilmformationinamexicanhospital AT martincjmaiden iacinetobacterbaumanniiiic2andic5isolateswithcoexistingiblaisuboxa143likesubandiblaisuboxa72subandexhibitingstrongbiofilmformationinamexicanhospital AT catalinagayossovazquez iacinetobacterbaumanniiiic2andic5isolateswithcoexistingiblaisuboxa143likesubandiblaisuboxa72subandexhibitingstrongbiofilmformationinamexicanhospital AT joseluisfernandezvazquez iacinetobacterbaumanniiiic2andic5isolateswithcoexistingiblaisuboxa143likesubandiblaisuboxa72subandexhibitingstrongbiofilmformationinamexicanhospital AT valeriamateoestrada iacinetobacterbaumanniiiic2andic5isolateswithcoexistingiblaisuboxa143likesubandiblaisuboxa72subandexhibitingstrongbiofilmformationinamexicanhospital AT silviagionocerezo iacinetobacterbaumanniiiic2andic5isolateswithcoexistingiblaisuboxa143likesubandiblaisuboxa72subandexhibitingstrongbiofilmformationinamexicanhospital AT mariadoloresalcantarcuriel iacinetobacterbaumanniiiic2andic5isolateswithcoexistingiblaisuboxa143likesubandiblaisuboxa72subandexhibitingstrongbiofilmformationinamexicanhospital |