Analysis of Epstein–Barr Virus (EBV) and PD-L1 Expression in Nasopharyngeal Carcinoma Patients in a Non-Endemic Region
Background: The purpose of this study was to evaluate the status of Epstein–Barr virus (EBV) infection and the expression of programmed cell death ligand-1 (PD-L1) in tumor samples from patients with nasopharyngeal carcinoma (NPC). Methods: Evaluation of EBV infection was performed through the detec...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11720 |
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| author | Josiane M. Dias Iara V. V. Santana Vinicius D. da Silva André L. Carvalho Lidia M. R. B. Arantes |
| author_facet | Josiane M. Dias Iara V. V. Santana Vinicius D. da Silva André L. Carvalho Lidia M. R. B. Arantes |
| author_sort | Josiane M. Dias |
| collection | DOAJ |
| description | Background: The purpose of this study was to evaluate the status of Epstein–Barr virus (EBV) infection and the expression of programmed cell death ligand-1 (PD-L1) in tumor samples from patients with nasopharyngeal carcinoma (NPC). Methods: Evaluation of EBV infection was performed through the detection of EBV-encoded small ribonucleic acids (EBER) by in situ hybridization, and PD-L1 expression was performed through immunohistochemistry. Results: In total, 124 samples were evaluated for EBER and 120 for PD-L1 expression. A total of 86.3% of cases were positive for EBER and 55.8% were positive for PD-L1. There was a correlation between EBER positivity and the presence of undifferentiated carcinoma histology (<i>p</i> = 0.007) as well as the absence of tobacco history (<i>p</i> = 0.019). There was a correlation between PD-L1 expression and EBER positivity (<i>p</i> = 0.004). There was no statistically significant difference between overall survival (OS) and EBER (<i>p</i> = 0.290) or PD-L1 (<i>p</i> = 0.801) expression. Conclusions: This study corresponds to one of the largest cohorts of NPC in a non-endemic region. Phase III studies with checkpoint inhibitors are ongoing and may provide more data about the role of PD-L1 expression in this disease. |
| first_indexed | 2024-03-09T21:37:59Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:37:59Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-227be15019ff4bb4a66ccbc8060d3ecc2023-11-23T20:38:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123191172010.3390/ijms231911720Analysis of Epstein–Barr Virus (EBV) and PD-L1 Expression in Nasopharyngeal Carcinoma Patients in a Non-Endemic RegionJosiane M. Dias0Iara V. V. Santana1Vinicius D. da Silva2André L. Carvalho3Lidia M. R. B. Arantes4Clinical Oncology Department, Barretos Cancer Hospital, Barretos 14784-400, SP, BrazilPathology Department, Barretos Cancer Hospital, Barretos 14784-400, SP, BrazilPathology Department, Barretos Cancer Hospital, Barretos 14784-400, SP, BrazilMolecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, SP, BrazilMolecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, SP, BrazilBackground: The purpose of this study was to evaluate the status of Epstein–Barr virus (EBV) infection and the expression of programmed cell death ligand-1 (PD-L1) in tumor samples from patients with nasopharyngeal carcinoma (NPC). Methods: Evaluation of EBV infection was performed through the detection of EBV-encoded small ribonucleic acids (EBER) by in situ hybridization, and PD-L1 expression was performed through immunohistochemistry. Results: In total, 124 samples were evaluated for EBER and 120 for PD-L1 expression. A total of 86.3% of cases were positive for EBER and 55.8% were positive for PD-L1. There was a correlation between EBER positivity and the presence of undifferentiated carcinoma histology (<i>p</i> = 0.007) as well as the absence of tobacco history (<i>p</i> = 0.019). There was a correlation between PD-L1 expression and EBER positivity (<i>p</i> = 0.004). There was no statistically significant difference between overall survival (OS) and EBER (<i>p</i> = 0.290) or PD-L1 (<i>p</i> = 0.801) expression. Conclusions: This study corresponds to one of the largest cohorts of NPC in a non-endemic region. Phase III studies with checkpoint inhibitors are ongoing and may provide more data about the role of PD-L1 expression in this disease.https://www.mdpi.com/1422-0067/23/19/11720nasopharyngeal carcinomaEpstein–Barr virus (EBV)programmed cell death ligand-1 (PD-L1)non-endemic region |
| spellingShingle | Josiane M. Dias Iara V. V. Santana Vinicius D. da Silva André L. Carvalho Lidia M. R. B. Arantes Analysis of Epstein–Barr Virus (EBV) and PD-L1 Expression in Nasopharyngeal Carcinoma Patients in a Non-Endemic Region International Journal of Molecular Sciences nasopharyngeal carcinoma Epstein–Barr virus (EBV) programmed cell death ligand-1 (PD-L1) non-endemic region |
| title | Analysis of Epstein–Barr Virus (EBV) and PD-L1 Expression in Nasopharyngeal Carcinoma Patients in a Non-Endemic Region |
| title_full | Analysis of Epstein–Barr Virus (EBV) and PD-L1 Expression in Nasopharyngeal Carcinoma Patients in a Non-Endemic Region |
| title_fullStr | Analysis of Epstein–Barr Virus (EBV) and PD-L1 Expression in Nasopharyngeal Carcinoma Patients in a Non-Endemic Region |
| title_full_unstemmed | Analysis of Epstein–Barr Virus (EBV) and PD-L1 Expression in Nasopharyngeal Carcinoma Patients in a Non-Endemic Region |
| title_short | Analysis of Epstein–Barr Virus (EBV) and PD-L1 Expression in Nasopharyngeal Carcinoma Patients in a Non-Endemic Region |
| title_sort | analysis of epstein barr virus ebv and pd l1 expression in nasopharyngeal carcinoma patients in a non endemic region |
| topic | nasopharyngeal carcinoma Epstein–Barr virus (EBV) programmed cell death ligand-1 (PD-L1) non-endemic region |
| url | https://www.mdpi.com/1422-0067/23/19/11720 |
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