A Comprehensive Comparative Study for the Authentication of the Kadsura Crude Drug
The stems and roots of Kadsura species have been used as the folk medicine in Traditional Chinese medicine (TCM) and have good traditional efficacy and medicinal application with a long history. Among these species, K. coccinea, K. heteroclita and K. longipedunculata are the most widely distributed...
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Frontiers Media S.A.
2019-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2018.01576/full |
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| author | Jiushi Liu Jiushi Liu Xueping Wei Xueping Wei Xiaoyi Zhang Xiaoyi Zhang Yaodong Qi Yaodong Qi Bengang Zhang Bengang Zhang Haitao Liu Haitao Liu Peigen Xiao Peigen Xiao |
| author_facet | Jiushi Liu Jiushi Liu Xueping Wei Xueping Wei Xiaoyi Zhang Xiaoyi Zhang Yaodong Qi Yaodong Qi Bengang Zhang Bengang Zhang Haitao Liu Haitao Liu Peigen Xiao Peigen Xiao |
| author_sort | Jiushi Liu |
| collection | DOAJ |
| description | The stems and roots of Kadsura species have been used as the folk medicine in Traditional Chinese medicine (TCM) and have good traditional efficacy and medicinal application with a long history. Among these species, K. coccinea, K. heteroclita and K. longipedunculata are the most widely distributed species in the regions of south and southwest China. Owing to their similar appearance, the crude drugs are often confusedly used by some folk doctors, even some pharmaceutical factories. To discriminate the crude drugs, haplotype analysis based on cpDNA markers and ITS was firstly employed in this study. Generic delimitation, interspecific interrelationships, and the identification of medicinal materials between K. longipedunculata and K. heteroclita remained unresolved by the existing molecular fragments. The original plant could be identified through the morphological character of flower, fruit and leaf. However, in most situation collectors have no chance to find out these characters due to lack of reproductive organs, and have no experience with the minor difference and transitional variation of leaf morphology. The chemical characterization show that the chemometric of chemical composition owned higher resolution to discriminate three herbs of Kadsura species. In conclusion, this integrative approach involving molecular phylogeny, morphology and chemical characterization could be applied for authentication of the Kadusra. Our study suggests the use of this comprehensive approach for accurate characterization of this closely related taxa as well as identifying the source plant and confused herbs of TCM. |
| first_indexed | 2024-04-12T10:58:42Z |
| format | Article |
| id | doaj.art-2280280f75214df6b13b3f113f3b5e11 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-12T10:58:42Z |
| publishDate | 2019-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-2280280f75214df6b13b3f113f3b5e112022-12-22T03:36:01ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122019-01-01910.3389/fphar.2018.01576409916A Comprehensive Comparative Study for the Authentication of the Kadsura Crude DrugJiushi Liu0Jiushi Liu1Xueping Wei2Xueping Wei3Xiaoyi Zhang4Xiaoyi Zhang5Yaodong Qi6Yaodong Qi7Bengang Zhang8Bengang Zhang9Haitao Liu10Haitao Liu11Peigen Xiao12Peigen Xiao13Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, ChinaKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Beijing, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, ChinaEngineering Research Center of Traditional Chinese Medicine Resources, Ministry of Education, Beijing, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, ChinaKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Beijing, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, ChinaEngineering Research Center of Traditional Chinese Medicine Resources, Ministry of Education, Beijing, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, ChinaEngineering Research Center of Traditional Chinese Medicine Resources, Ministry of Education, Beijing, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, ChinaKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Beijing, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, ChinaKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Beijing, ChinaThe stems and roots of Kadsura species have been used as the folk medicine in Traditional Chinese medicine (TCM) and have good traditional efficacy and medicinal application with a long history. Among these species, K. coccinea, K. heteroclita and K. longipedunculata are the most widely distributed species in the regions of south and southwest China. Owing to their similar appearance, the crude drugs are often confusedly used by some folk doctors, even some pharmaceutical factories. To discriminate the crude drugs, haplotype analysis based on cpDNA markers and ITS was firstly employed in this study. Generic delimitation, interspecific interrelationships, and the identification of medicinal materials between K. longipedunculata and K. heteroclita remained unresolved by the existing molecular fragments. The original plant could be identified through the morphological character of flower, fruit and leaf. However, in most situation collectors have no chance to find out these characters due to lack of reproductive organs, and have no experience with the minor difference and transitional variation of leaf morphology. The chemical characterization show that the chemometric of chemical composition owned higher resolution to discriminate three herbs of Kadsura species. In conclusion, this integrative approach involving molecular phylogeny, morphology and chemical characterization could be applied for authentication of the Kadusra. Our study suggests the use of this comprehensive approach for accurate characterization of this closely related taxa as well as identifying the source plant and confused herbs of TCM.https://www.frontiersin.org/article/10.3389/fphar.2018.01576/fullKadsuramorphologymolecular markerschemical characterizationidentification |
| spellingShingle | Jiushi Liu Jiushi Liu Xueping Wei Xueping Wei Xiaoyi Zhang Xiaoyi Zhang Yaodong Qi Yaodong Qi Bengang Zhang Bengang Zhang Haitao Liu Haitao Liu Peigen Xiao Peigen Xiao A Comprehensive Comparative Study for the Authentication of the Kadsura Crude Drug Frontiers in Pharmacology Kadsura morphology molecular markers chemical characterization identification |
| title | A Comprehensive Comparative Study for the Authentication of the Kadsura Crude Drug |
| title_full | A Comprehensive Comparative Study for the Authentication of the Kadsura Crude Drug |
| title_fullStr | A Comprehensive Comparative Study for the Authentication of the Kadsura Crude Drug |
| title_full_unstemmed | A Comprehensive Comparative Study for the Authentication of the Kadsura Crude Drug |
| title_short | A Comprehensive Comparative Study for the Authentication of the Kadsura Crude Drug |
| title_sort | comprehensive comparative study for the authentication of the kadsura crude drug |
| topic | Kadsura morphology molecular markers chemical characterization identification |
| url | https://www.frontiersin.org/article/10.3389/fphar.2018.01576/full |
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