Cryostructuring of Polymeric Systems: 63. Synthesis of Two Chemically Tanned Gelatin-Based Cryostructurates and Evaluation of Their Potential as Scaffolds for Culturing of Mammalian Cells
Various gelatin-containing gel materials are used as scaffolds for animal and human cell culturing within the fields of cell technologies and tissue engineering. Cryostructuring is a promising technique for the preparation of efficient macroporous scaffolds in biomedical applications. In the current...
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2022-10-01
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| author | Vladimir I. Lozinsky Valentina K. Kulakova Alexei M. Grigoriev Elena A. Podorozhko Ludmila A. Kirsanova Aleksandra D. Kirillova Ivan A. Novikov Yulia B. Basok Viktor I. Sevastianov |
| author_facet | Vladimir I. Lozinsky Valentina K. Kulakova Alexei M. Grigoriev Elena A. Podorozhko Ludmila A. Kirsanova Aleksandra D. Kirillova Ivan A. Novikov Yulia B. Basok Viktor I. Sevastianov |
| author_sort | Vladimir I. Lozinsky |
| collection | DOAJ |
| description | Various gelatin-containing gel materials are used as scaffolds for animal and human cell culturing within the fields of cell technologies and tissue engineering. Cryostructuring is a promising technique for the preparation of efficient macroporous scaffolds in biomedical applications. In the current study, two new gelatin-based cryostructurates were synthesized, their physicochemical properties and microstructure were evaluated, and their ability to serve as biocompatible scaffolds for mammalian cells culturing was tested. The preparation procedure included the dissolution of Type A gelatin in water, the addition of urea to inhibit self-gelation, the freezing of such a solution, ice sublimation in vacuo, and urea extraction with ethanol from the freeze-dried matter followed by its cross-linking in an ethanol medium with either carbodiimide or glyoxal. It was shown that in the former case, a denser cross-linked polymer phase was formed, while in the latter case, the macropores in the resultant biopolymer material were wider. The subsequent biotesting of these scaffolds demonstrated their biocompatibility for human mesenchymal stromal cells and HepG2 cells during subcutaneous implantation in rats. Albumin secretion and urea synthesis by HepG2 cells confirmed the possibility of using gelatin cryostructurates for liver tissue engineering. |
| first_indexed | 2024-03-09T19:03:14Z |
| format | Article |
| id | doaj.art-2286ea97e8274bff84f141bca4e84188 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T19:03:14Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-2286ea97e8274bff84f141bca4e841882023-11-24T04:46:15ZengMDPI AGGels2310-28612022-10-0181169510.3390/gels8110695Cryostructuring of Polymeric Systems: 63. Synthesis of Two Chemically Tanned Gelatin-Based Cryostructurates and Evaluation of Their Potential as Scaffolds for Culturing of Mammalian CellsVladimir I. Lozinsky0Valentina K. Kulakova1Alexei M. Grigoriev2Elena A. Podorozhko3Ludmila A. Kirsanova4Aleksandra D. Kirillova5Ivan A. Novikov6Yulia B. Basok7Viktor I. Sevastianov8A.N.Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilov Street 28, Bld. 1, 119334 Moscow, RussiaA.N.Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilov Street 28, Bld. 1, 119334 Moscow, RussiaV.I.Shumakov Federal Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation, Shchukinskaya Street 1, 123182 Moscow, RussiaA.N.Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Vavilov Street 28, Bld. 1, 119334 Moscow, RussiaV.I.Shumakov Federal Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation, Shchukinskaya Street 1, 123182 Moscow, RussiaV.I.Shumakov Federal Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation, Shchukinskaya Street 1, 123182 Moscow, RussiaScientific Research Institute of Eye Diseases, Rossolimo Street 11A, 119021 Moscow, RussiaV.I.Shumakov Federal Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation, Shchukinskaya Street 1, 123182 Moscow, RussiaV.I.Shumakov Federal Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation, Shchukinskaya Street 1, 123182 Moscow, RussiaVarious gelatin-containing gel materials are used as scaffolds for animal and human cell culturing within the fields of cell technologies and tissue engineering. Cryostructuring is a promising technique for the preparation of efficient macroporous scaffolds in biomedical applications. In the current study, two new gelatin-based cryostructurates were synthesized, their physicochemical properties and microstructure were evaluated, and their ability to serve as biocompatible scaffolds for mammalian cells culturing was tested. The preparation procedure included the dissolution of Type A gelatin in water, the addition of urea to inhibit self-gelation, the freezing of such a solution, ice sublimation in vacuo, and urea extraction with ethanol from the freeze-dried matter followed by its cross-linking in an ethanol medium with either carbodiimide or glyoxal. It was shown that in the former case, a denser cross-linked polymer phase was formed, while in the latter case, the macropores in the resultant biopolymer material were wider. The subsequent biotesting of these scaffolds demonstrated their biocompatibility for human mesenchymal stromal cells and HepG2 cells during subcutaneous implantation in rats. Albumin secretion and urea synthesis by HepG2 cells confirmed the possibility of using gelatin cryostructurates for liver tissue engineering.https://www.mdpi.com/2310-2861/8/11/695gelatincryostructuringcarbodiimideglyoxalcell culture scaffoldsin vitro and in vivo bioassay |
| spellingShingle | Vladimir I. Lozinsky Valentina K. Kulakova Alexei M. Grigoriev Elena A. Podorozhko Ludmila A. Kirsanova Aleksandra D. Kirillova Ivan A. Novikov Yulia B. Basok Viktor I. Sevastianov Cryostructuring of Polymeric Systems: 63. Synthesis of Two Chemically Tanned Gelatin-Based Cryostructurates and Evaluation of Their Potential as Scaffolds for Culturing of Mammalian Cells Gels gelatin cryostructuring carbodiimide glyoxal cell culture scaffolds in vitro and in vivo bioassay |
| title | Cryostructuring of Polymeric Systems: 63. Synthesis of Two Chemically Tanned Gelatin-Based Cryostructurates and Evaluation of Their Potential as Scaffolds for Culturing of Mammalian Cells |
| title_full | Cryostructuring of Polymeric Systems: 63. Synthesis of Two Chemically Tanned Gelatin-Based Cryostructurates and Evaluation of Their Potential as Scaffolds for Culturing of Mammalian Cells |
| title_fullStr | Cryostructuring of Polymeric Systems: 63. Synthesis of Two Chemically Tanned Gelatin-Based Cryostructurates and Evaluation of Their Potential as Scaffolds for Culturing of Mammalian Cells |
| title_full_unstemmed | Cryostructuring of Polymeric Systems: 63. Synthesis of Two Chemically Tanned Gelatin-Based Cryostructurates and Evaluation of Their Potential as Scaffolds for Culturing of Mammalian Cells |
| title_short | Cryostructuring of Polymeric Systems: 63. Synthesis of Two Chemically Tanned Gelatin-Based Cryostructurates and Evaluation of Their Potential as Scaffolds for Culturing of Mammalian Cells |
| title_sort | cryostructuring of polymeric systems 63 synthesis of two chemically tanned gelatin based cryostructurates and evaluation of their potential as scaffolds for culturing of mammalian cells |
| topic | gelatin cryostructuring carbodiimide glyoxal cell culture scaffolds in vitro and in vivo bioassay |
| url | https://www.mdpi.com/2310-2861/8/11/695 |
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