Effect of Silica Xerogel Functionalization on Intensification of <i>Rindera graeca</i> Transgenic Roots Proliferation and Boosting Naphthoquinone Production
Secondary metabolites derived from plants are recognized as valuable products with several successful applications in the pharmaceutical, cosmetic, and food industries. The major limitation to the broader implementation of these compounds is their low manufacturing efficiency. Current efforts to ove...
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MDPI AG
2024-01-01
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| Online Access: | https://www.mdpi.com/2075-1729/14/1/159 |
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| author | Kamil Wierzchowski Bartosz Nowak Mateusz Kawka Katarzyna Sykłowska-Baranek Maciej Pilarek |
| author_facet | Kamil Wierzchowski Bartosz Nowak Mateusz Kawka Katarzyna Sykłowska-Baranek Maciej Pilarek |
| author_sort | Kamil Wierzchowski |
| collection | DOAJ |
| description | Secondary metabolites derived from plants are recognized as valuable products with several successful applications in the pharmaceutical, cosmetic, and food industries. The major limitation to the broader implementation of these compounds is their low manufacturing efficiency. Current efforts to overcome unprofitability depend mainly on biotechnological methods, especially through the application of plant in vitro cultures. This concept allows unprecedented bioengineering opportunities for culture system modifications with in situ product removal. The silica-based xerogels can be used as a novel, porous biomaterial characterized by a large surface area and high affinity to lipophilic secondary metabolites produced by plant tissue. This study aimed to investigate the influence of xerogel-based biomaterials functionalized with methyl, hydroxyl, carboxylic, and amine groups on <i>Rindera graeca</i> transgenic root growth and the production of naphthoquinone derivatives. The application of xerogel-based scaffolds functionalized with the methyl group resulted in more than 1.5 times higher biomass proliferation than for reference untreated culture. The naphthoquinone derivatives’ production was noted exclusively in culture systems supplemented with xerogel functionalized with methyl and hydroxyl groups. Applying chemically functionalized xerogels as in situ adsorbents allowed for the enhanced growth and productivity of in vitro cultured <i>R. graeca</i> transgenic roots, facilitating product isolation due to their selective and efficient accumulation. |
| first_indexed | 2024-03-08T10:42:59Z |
| format | Article |
| id | doaj.art-2288fa9a2f064a6bae13e36b86a800ad |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-08T10:42:59Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-2288fa9a2f064a6bae13e36b86a800ad2024-01-26T17:21:55ZengMDPI AGLife2075-17292024-01-0114115910.3390/life14010159Effect of Silica Xerogel Functionalization on Intensification of <i>Rindera graeca</i> Transgenic Roots Proliferation and Boosting Naphthoquinone ProductionKamil Wierzchowski0Bartosz Nowak1Mateusz Kawka2Katarzyna Sykłowska-Baranek3Maciej Pilarek4Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, PolandFaculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, PolandDepartment of Pharmaceutical Biology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandDepartment of Pharmaceutical Biology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, PolandFaculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, PolandSecondary metabolites derived from plants are recognized as valuable products with several successful applications in the pharmaceutical, cosmetic, and food industries. The major limitation to the broader implementation of these compounds is their low manufacturing efficiency. Current efforts to overcome unprofitability depend mainly on biotechnological methods, especially through the application of plant in vitro cultures. This concept allows unprecedented bioengineering opportunities for culture system modifications with in situ product removal. The silica-based xerogels can be used as a novel, porous biomaterial characterized by a large surface area and high affinity to lipophilic secondary metabolites produced by plant tissue. This study aimed to investigate the influence of xerogel-based biomaterials functionalized with methyl, hydroxyl, carboxylic, and amine groups on <i>Rindera graeca</i> transgenic root growth and the production of naphthoquinone derivatives. The application of xerogel-based scaffolds functionalized with the methyl group resulted in more than 1.5 times higher biomass proliferation than for reference untreated culture. The naphthoquinone derivatives’ production was noted exclusively in culture systems supplemented with xerogel functionalized with methyl and hydroxyl groups. Applying chemically functionalized xerogels as in situ adsorbents allowed for the enhanced growth and productivity of in vitro cultured <i>R. graeca</i> transgenic roots, facilitating product isolation due to their selective and efficient accumulation.https://www.mdpi.com/2075-1729/14/1/159transgenic (hairy) rootsxerogelin situ extractionplant secondary metabolites |
| spellingShingle | Kamil Wierzchowski Bartosz Nowak Mateusz Kawka Katarzyna Sykłowska-Baranek Maciej Pilarek Effect of Silica Xerogel Functionalization on Intensification of <i>Rindera graeca</i> Transgenic Roots Proliferation and Boosting Naphthoquinone Production Life transgenic (hairy) roots xerogel in situ extraction plant secondary metabolites |
| title | Effect of Silica Xerogel Functionalization on Intensification of <i>Rindera graeca</i> Transgenic Roots Proliferation and Boosting Naphthoquinone Production |
| title_full | Effect of Silica Xerogel Functionalization on Intensification of <i>Rindera graeca</i> Transgenic Roots Proliferation and Boosting Naphthoquinone Production |
| title_fullStr | Effect of Silica Xerogel Functionalization on Intensification of <i>Rindera graeca</i> Transgenic Roots Proliferation and Boosting Naphthoquinone Production |
| title_full_unstemmed | Effect of Silica Xerogel Functionalization on Intensification of <i>Rindera graeca</i> Transgenic Roots Proliferation and Boosting Naphthoquinone Production |
| title_short | Effect of Silica Xerogel Functionalization on Intensification of <i>Rindera graeca</i> Transgenic Roots Proliferation and Boosting Naphthoquinone Production |
| title_sort | effect of silica xerogel functionalization on intensification of i rindera graeca i transgenic roots proliferation and boosting naphthoquinone production |
| topic | transgenic (hairy) roots xerogel in situ extraction plant secondary metabolites |
| url | https://www.mdpi.com/2075-1729/14/1/159 |
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