Determination of selected phenolic acid and majoritarian avenanthramides in different varieties of naked oats (Avena sativa L.) grown in Slovakia
Oats are important cereals. Oats are a good source of protein and lipids, polyphenolics, phenolic acids, flavonoids and avenanthramides. Avenanthramides is phenolic group, which is unique in oats and have antioxidant activity, anti-inflammatory, anti-atherogenic and anti-proliferative effect. The ai...
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| Format: | Article |
| Language: | English |
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SciCell s.r.o.
2018-12-01
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| Series: | Nova Biotechnologica et Chimica |
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| Online Access: | http://www.degruyter.com/view/j/nbec.2018.17.issue-2/nbec-2018-0014/nbec-2018-0014.xml?format=INT |
| _version_ | 1818200066693791744 |
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| author | Kulichová Katarína Maliarová Mária Sokol Jozef Lašáková Katarína Havrlentová Michaela |
| author_facet | Kulichová Katarína Maliarová Mária Sokol Jozef Lašáková Katarína Havrlentová Michaela |
| author_sort | Kulichová Katarína |
| collection | DOAJ |
| description | Oats are important cereals. Oats are a good source of protein and lipids, polyphenolics, phenolic acids, flavonoids and avenanthramides. Avenanthramides is phenolic group, which is unique in oats and have antioxidant activity, anti-inflammatory, anti-atherogenic and anti-proliferative effect. The aim of study is determination of the majoritarian avenanthramides (2c, 2p and 2f) and phenolic acids (p-coumaric and ferulic) in selected varieties of oat (Avena sativa L.) grown in two consecutive years using the HPLC method. The oats were exposed to ultrasound supported extraction (two 15 min cycles).The simultaneous separation was performed using C18 type of stationary phase. The method showed a good linearity in the concentration range 0.04 - 5.24 μg/mL for p-coumaric acid, 0.04 - 5.13 μg/mL for ferulic acid, 0.19 - 24.5 μg/mL for avenanthramide 2c, 0.53 - 17.1 μg/mL for avenanthramide 2p, 0.8 - 25.6 μg/mL for avenanthramide 2f. Correlation coefficients were higher than 0.9997. Detector operated at a wavelength 320 nm. The repeatability of the method was evaluated in three concentration levels with satisfactory results for each analyte. The content of both phenolic acids is significantly lower (50- - 100-times) compared to the total content of avenanthramides in both years’ harvests for all analyzed varieties. Content of total avenanthramides was the highest in varieties Racoon (723.28 mg/kg) followed by Oliver (578.59 mg/kg) and Kamil (384.17 mg/kg). |
| first_indexed | 2024-12-12T02:31:45Z |
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| id | doaj.art-22949114323443428492b25508a8883a |
| institution | Directory Open Access Journal |
| issn | 1339-004X |
| language | English |
| last_indexed | 2024-12-12T02:31:45Z |
| publishDate | 2018-12-01 |
| publisher | SciCell s.r.o. |
| record_format | Article |
| series | Nova Biotechnologica et Chimica |
| spelling | doaj.art-22949114323443428492b25508a8883a2022-12-22T00:41:22ZengSciCell s.r.o.Nova Biotechnologica et Chimica1339-004X2018-12-0117213213910.2478/nbec-2018-0014nbec-2018-0014Determination of selected phenolic acid and majoritarian avenanthramides in different varieties of naked oats (Avena sativa L.) grown in SlovakiaKulichová Katarína0Maliarová Mária1Sokol Jozef2Lašáková Katarína3Havrlentová Michaela4Department of Chemistry, Faculty of Natural Sciences, University of SS. Cyril and Methodius in Trnava, Nám. J. Herdu 2,Trnava, 917 01, Slovak RepublicDepartment of Chemistry, Faculty of Natural Sciences, University of SS. Cyril and Methodius in Trnava, Nám. J. Herdu 2,Trnava, 917 01, Slovak RepublicDepartment of Chemistry, Faculty of Natural Sciences, University of SS. Cyril and Methodius in Trnava, Nám. J. Herdu 2,Trnava, 917 01, Slovak RepublicDepartment of Chemistry, Faculty of Natural Sciences, University of SS. Cyril and Methodius in Trnava, Nám. J. Herdu 2,Trnava, 917 01, Slovak RepublicDepartment of Biotechnologies, Faculty of Natural Sciences, University of Ss. Cyril and Methodius in Trnava, Nám. J. Herdu 2,Trnava, 917 01, Slovak RepublicOats are important cereals. Oats are a good source of protein and lipids, polyphenolics, phenolic acids, flavonoids and avenanthramides. Avenanthramides is phenolic group, which is unique in oats and have antioxidant activity, anti-inflammatory, anti-atherogenic and anti-proliferative effect. The aim of study is determination of the majoritarian avenanthramides (2c, 2p and 2f) and phenolic acids (p-coumaric and ferulic) in selected varieties of oat (Avena sativa L.) grown in two consecutive years using the HPLC method. The oats were exposed to ultrasound supported extraction (two 15 min cycles).The simultaneous separation was performed using C18 type of stationary phase. The method showed a good linearity in the concentration range 0.04 - 5.24 μg/mL for p-coumaric acid, 0.04 - 5.13 μg/mL for ferulic acid, 0.19 - 24.5 μg/mL for avenanthramide 2c, 0.53 - 17.1 μg/mL for avenanthramide 2p, 0.8 - 25.6 μg/mL for avenanthramide 2f. Correlation coefficients were higher than 0.9997. Detector operated at a wavelength 320 nm. The repeatability of the method was evaluated in three concentration levels with satisfactory results for each analyte. The content of both phenolic acids is significantly lower (50- - 100-times) compared to the total content of avenanthramides in both years’ harvests for all analyzed varieties. Content of total avenanthramides was the highest in varieties Racoon (723.28 mg/kg) followed by Oliver (578.59 mg/kg) and Kamil (384.17 mg/kg).http://www.degruyter.com/view/j/nbec.2018.17.issue-2/nbec-2018-0014/nbec-2018-0014.xml?format=INTHPLC -DAD Avenanthramides Phenolic Acids Oats |
| spellingShingle | Kulichová Katarína Maliarová Mária Sokol Jozef Lašáková Katarína Havrlentová Michaela Determination of selected phenolic acid and majoritarian avenanthramides in different varieties of naked oats (Avena sativa L.) grown in Slovakia Nova Biotechnologica et Chimica HPLC -DAD Avenanthramides Phenolic Acids Oats |
| title | Determination of selected phenolic acid and majoritarian avenanthramides in different varieties of naked oats (Avena sativa L.) grown in Slovakia |
| title_full | Determination of selected phenolic acid and majoritarian avenanthramides in different varieties of naked oats (Avena sativa L.) grown in Slovakia |
| title_fullStr | Determination of selected phenolic acid and majoritarian avenanthramides in different varieties of naked oats (Avena sativa L.) grown in Slovakia |
| title_full_unstemmed | Determination of selected phenolic acid and majoritarian avenanthramides in different varieties of naked oats (Avena sativa L.) grown in Slovakia |
| title_short | Determination of selected phenolic acid and majoritarian avenanthramides in different varieties of naked oats (Avena sativa L.) grown in Slovakia |
| title_sort | determination of selected phenolic acid and majoritarian avenanthramides in different varieties of naked oats avena sativa l grown in slovakia |
| topic | HPLC -DAD Avenanthramides Phenolic Acids Oats |
| url | http://www.degruyter.com/view/j/nbec.2018.17.issue-2/nbec-2018-0014/nbec-2018-0014.xml?format=INT |
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