The Uneven Distribution of Mating Type Genes in Natural and Cultivated Truffle Orchards Contributes to the Fructification of Tuber indicum
The aim of this study was to investigate the pattern of distribution of mating type (MAT) genes of Tuber indicum in ectomycorhizosphere soils from natural T. indicum-producing areas and cultivated truffle orchards and ascocarp samples from different regions. Quantitative real-time PCR and multiplex...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Mycobiology |
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| Online Access: | http://dx.doi.org/10.1080/12298093.2018.1454005 |
| _version_ | 1818931660036505600 |
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| author | Qiang Li Yu Fu Qun Sun Pierre Sourzat Mei Yang Chengyi Liu Hao Tan Lei Ye Jie Zou Chenguang Wu Bo Zhang Xiaolin Li |
| author_facet | Qiang Li Yu Fu Qun Sun Pierre Sourzat Mei Yang Chengyi Liu Hao Tan Lei Ye Jie Zou Chenguang Wu Bo Zhang Xiaolin Li |
| author_sort | Qiang Li |
| collection | DOAJ |
| description | The aim of this study was to investigate the pattern of distribution of mating type (MAT) genes of Tuber indicum in ectomycorhizosphere soils from natural T. indicum-producing areas and cultivated truffle orchards and ascocarp samples from different regions. Quantitative real-time PCR and multiplex PCR were used to weight the copy numbers of MAT1-1-1 and MAT1-2-1 in natural truffle soils and cultivated orchard soils. The effect of limestone on the pattern of truffle MAT genes and the correlation between soil properties and the proportion of MAT genes were also assessed. These results indicated that an uneven and nonrandom distribution of MAT genes was common in truffle-producing areas, cultivated truffle orchards, and ascocarps gleba. The competition between the two mating type genes and the expansion of unbalanced distribution was found to be closely related to truffle fructification. Limestone treatments failed to alter the proportion of the two mating type genes in the soil. The content of available phosphorus in soil was significantly correlated with the value of MAT1-1-1/MAT1-2-1 in cultivated and natural ectomycorhizosphere soils. The application of real-time quantitative PCR can provide reference for monitoring the dynamic changes of mating type genes in soil. This study investigates the distributional pattern of T. indicum MAT genes in the ectomycorhizosphere soil and ascocarp gleba from different regions, which may provide a foundation for the cultivation of T. indicum. |
| first_indexed | 2024-12-20T04:20:07Z |
| format | Article |
| id | doaj.art-229532d1d0ca4028ba989f66ebfd00f2 |
| institution | Directory Open Access Journal |
| issn | 1229-8093 2092-9323 |
| language | English |
| last_indexed | 2024-12-20T04:20:07Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycobiology |
| spelling | doaj.art-229532d1d0ca4028ba989f66ebfd00f22022-12-21T19:53:39ZengTaylor & Francis GroupMycobiology1229-80932092-93232018-01-01461647110.1080/12298093.2018.14540051454005The Uneven Distribution of Mating Type Genes in Natural and Cultivated Truffle Orchards Contributes to the Fructification of Tuber indicumQiang Li0Yu Fu1Qun Sun2Pierre Sourzat3Mei Yang4Chengyi Liu5Hao Tan6Lei Ye7Jie Zou8Chenguang Wu9Bo Zhang10Xiaolin Li11Sichuan Academy of Agricultural SciencesSichuan Academy of Agricultural SciencesSichuan UniversityTruffle Research Centre of Cahors-Le MontatPanzhihua Academy of Agricultural and Forestry SciencesPanzhihua Academy of Agricultural and Forestry SciencesSichuan Academy of Agricultural SciencesSichuan Academy of Agricultural SciencesSichuan Academy of Agricultural SciencesSichuan Academy of Agricultural SciencesSichuan Academy of Agricultural SciencesSichuan Academy of Agricultural SciencesThe aim of this study was to investigate the pattern of distribution of mating type (MAT) genes of Tuber indicum in ectomycorhizosphere soils from natural T. indicum-producing areas and cultivated truffle orchards and ascocarp samples from different regions. Quantitative real-time PCR and multiplex PCR were used to weight the copy numbers of MAT1-1-1 and MAT1-2-1 in natural truffle soils and cultivated orchard soils. The effect of limestone on the pattern of truffle MAT genes and the correlation between soil properties and the proportion of MAT genes were also assessed. These results indicated that an uneven and nonrandom distribution of MAT genes was common in truffle-producing areas, cultivated truffle orchards, and ascocarps gleba. The competition between the two mating type genes and the expansion of unbalanced distribution was found to be closely related to truffle fructification. Limestone treatments failed to alter the proportion of the two mating type genes in the soil. The content of available phosphorus in soil was significantly correlated with the value of MAT1-1-1/MAT1-2-1 in cultivated and natural ectomycorhizosphere soils. The application of real-time quantitative PCR can provide reference for monitoring the dynamic changes of mating type genes in soil. This study investigates the distributional pattern of T. indicum MAT genes in the ectomycorhizosphere soil and ascocarp gleba from different regions, which may provide a foundation for the cultivation of T. indicum.http://dx.doi.org/10.1080/12298093.2018.1454005AscocarpectomycorrhizaMAT geneQ-PCRTuber indicum |
| spellingShingle | Qiang Li Yu Fu Qun Sun Pierre Sourzat Mei Yang Chengyi Liu Hao Tan Lei Ye Jie Zou Chenguang Wu Bo Zhang Xiaolin Li The Uneven Distribution of Mating Type Genes in Natural and Cultivated Truffle Orchards Contributes to the Fructification of Tuber indicum Mycobiology Ascocarp ectomycorrhiza MAT gene Q-PCR Tuber indicum |
| title | The Uneven Distribution of Mating Type Genes in Natural and Cultivated Truffle Orchards Contributes to the Fructification of Tuber indicum |
| title_full | The Uneven Distribution of Mating Type Genes in Natural and Cultivated Truffle Orchards Contributes to the Fructification of Tuber indicum |
| title_fullStr | The Uneven Distribution of Mating Type Genes in Natural and Cultivated Truffle Orchards Contributes to the Fructification of Tuber indicum |
| title_full_unstemmed | The Uneven Distribution of Mating Type Genes in Natural and Cultivated Truffle Orchards Contributes to the Fructification of Tuber indicum |
| title_short | The Uneven Distribution of Mating Type Genes in Natural and Cultivated Truffle Orchards Contributes to the Fructification of Tuber indicum |
| title_sort | uneven distribution of mating type genes in natural and cultivated truffle orchards contributes to the fructification of tuber indicum |
| topic | Ascocarp ectomycorrhiza MAT gene Q-PCR Tuber indicum |
| url | http://dx.doi.org/10.1080/12298093.2018.1454005 |
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